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Palaz Esra Bulunuz,Demirel Fatih,Adali Sümeyye,Demirel Serap,Yilmaz Abdurrahim 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2
Orchids are naturally grown in many countries of the Eastern Mediterranean. Salep, produced from orchid tubers via grinding and used as a hot drink, is an essential ingredient of ice cream. Salep orchid species are in danger of extinction due to the absence of cultivation and over-harvesting from nature. In this study, the genetic diversity and population structure between salep orchid species, their hybrids, and commercial species of Phalenopsis sp. were first investigated using inter-Primer Binding Site (iPBS) Retrotransposon markers. A total of 854 bands were scored with a 100% polymorphism rate. Neighbor-joining, model-based structure, and PCoA (Principal Coordinate Analysis) algorithms clustered the 30 salep orchids into three main populations. The analysis of molecular variance revealed variations within and among the populations as 71% and 29%, respectively. Anacamptis morio and Serapias vomeracea had the furthest genetic distances, and F1 hybrids of S. vomeracea and A. morio had great genetic diversity. The study results will provide helpful information for orchid breeding by eliciting the genetic distances of salep orchids.
Granulosa cell proliferation is inhibited by PGE2 in the primate ovulatory follicle
Patric S. Lundberg,Gil J. Moskowitz,Carmel Bellacose,Esra Demirel,Heidi A. Trau,Diane M. Duffy 한국통합생물학회 2020 Animal cells and systems Vol.24 No.3
Prostaglandin E2 (PGE2) is a key paracrine mediator of ovulation. Few specific PGE2-regulated gene products have been identified, so we hypothesized that PGE2 may regulate the expression and/or activity of a network of proteins to promote ovulation. To test this concept, Ingenuity Pathway Analysis (IPA) was used to predict PGE2-regulated functionalities in the primate ovulatory follicle. Cynomolgus macaques underwent ovarian stimulation. Follicular granulosa cells were obtained before (0 h) or 36 h after an ovulatory dose of human chorionic gonadotropin (hCG), with ovulation anticipated 37–40 h after hCG. Granulosa cells were obtained from additional monkeys 36 h after treatment with hCG and the PTGS2 inhibitor celecoxib, which significantly reduced hCG-stimulated follicular prostaglandin synthesis. Granulosa cell RNA expression was determined by microarray and analyzed using IPA. No granulosa cell mRNAs were identified as being significantly up-regulated or down-regulated by hCG + celecoxib compared with hCG only. However, IPA predicted that prostaglandin depletion significantly regulated several functional pathways. Cell cycle/cell proliferation was selected for further study because decreased granulosa cell proliferation is known to be necessary for ovulation and formation of a fullyfunctional corpus luteum. Prospective in vivo and in vitro experiments confirmed the prediction that hCG-stimulated cessation of granulosa cell proliferation is mediated via PGE2. Our studies indicate that PGE2 provides critical regulation of granulosa cell proliferation through mechanisms that do not involve significant regulation of mRNA levels of key cell cycle regulators. Pathway analysis correctly predicted that PGE2 serves as a paracrine mediator of this important transition in ovarian structure and function.