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Victor E. Balderas Hernandez,Luz M. T. Paz Maldonado,Emilio Medina Rivero,Ana P. Barba de la Rosa,Leandro G. Ordonez Acevedo,Antonio De Leon Rodriguez 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.1
The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were temperature, biomass concentration at induction time and the NaCl concentration as inducer. According to the Box-Behnken design, a second order response function was developed. The optimal expression conditions were a temperature of 32.6℃, induction biomass of 0.31 g/L and 0.3 M NaCl in minimal medium. The model prediction for the maximum hIFN-γ production was 77.3 mg/L, which corresponded satisfactorily with the experimental data. The hIFN-γ concentration attained under optimized conditions was 13-times higher than that obtained using the non-optimized conditions. We conclude that RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in E. coli.
Swine Dialyzable Spleen Extract as Antiviral Prophylaxis
Giovanna Merchand-Reyes,Lenin Pavon,Gilberto Pe´rez-Sa´nchez,Said Va´zquez-Leyva,Nohemı´ Salinas-Jazmı´n,,Marco Velasco-Vela´zquez,Emilio Medina-Rivero,Sonia Mayra Pe´rez-Tapia 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.11
Worldwide, the most highly consumed meat is of porcine origin. The production and distribution of swine meat are affected by diverse health matters, such as influenza and diarrhea, which cause head losses and require the use of antibiotics and other drugs in hog farms. To stimulate newborn piglet immune responses and increase resistance to infections, we developed a spray-drying technique to produce dried swine dialyzable spleen extract (sDSE), an immunomodulator. Based on the size-exclusion ultra performance liquid chromatography quantitative analysis, it was possible to recover up to 58% of the product after the drying process. The biological activity of orally administered dried sDSE increased mouse survival and induced cytokine production in a herpes infection model.