A Synthetic Naringenin Derivative, 5-Hydroxy-7,4′-diacetyloxyflavanone-<i>N</i>-phenyl Hydrazone (N101-43), Induces Apoptosis through Up-regulation of Fas/FasL Expression and Inhibition of PI3K/Akt Signaling Pathways in Non-Small-Cell Lung Cancer Cells

Bak, Yesol,Kim, Heejong,Kang, Jeong-Woo,Lee, Dong Hun,Kim, Man Sub,Park, Yun Sun,Kim, Jung-Hee,Jung, Kang-Yeoun,Lim, Yoongho,Hong, Jintae,Yoon, Do-Young American Chemical Society 2011 Journal of agricultural and food chemistry Vol.59 No.18

<P>Naringenin, a well-known naturally occurring flavonone, demonstrates cytotoxicity in a variety of human cancer cell lines; its inhibitory effects on tumor growth have spurred interest in its therapeutic application. In this study, naringenin was derivatized to produce more effective small-molecule inhibitors of cancer cell proliferation, and the anticancer effects of its derivative, 5-hydroxy-7,4′-diacetyloxyflavanone-<I>N</I>-phenyl hydrazone (N101-43), in non-small-cell lung cancer (NSCLC) cell lines NCI-H460, A549, and NCI-H1299 were investigated. Naringenin itself possesses no cytotoxicity against lung cancer cells. In contrast, N101-43 inhibits proliferation of both NCI-H460 and A549 cell lines; this capacity is lost in p53-lacking NCI-H1299 cells. N101-43 induces apoptosis via sub-G<SUB>1</SUB> cell-cycle arrest in NCI-H460 and via G<SUB>0</SUB>/G<SUB>1</SUB> arrest in A549 cells. Expression of apoptosis and cell-cycle regulatory factors is altered: Cyclins A and D1 and phospho-pRb are down-regulated, but expression of CDK inhibitors such as p21, p27, and p53 is enhanced by N101-43 treatment; N101-43 also increases expression levels of the extrinsic death receptor Fas and its binding partner FasL. Furthermore, N101-43 treatment diminishes levels of cell survival factors such as PI3K and p-Akt dose-dependently, and N101-43 additionally induces cleavage of the pro-apoptotic factors caspase-3, caspase-8, and poly ADP-ribose polymerase (PARP). Cumulatively, these investigations show that the naringenin derivative N101-43 induces apoptosis via up-regulation of Fas/FasL expression, activation of caspase cascades, and inhibition of PI3K/Akt survival signaling pathways in NCI-H460 and A549 cells. In conclusion, these data indicate that N101-43 may have potential as an anticancer agent in NSCLC.</P>

Shelf Life Determination of Precooked Frozen Meat Dumplings

Lee, Young-Chun,Yang, Hyung-Sub,Kim, Dong-Ho 한국산업식품공학회 2002 산업 식품공학 Vol.6 No.3

Quality of frozen foods is significantly affected by the storage temperature and tie, and the consumers' demand for safe quality frozen foods is increasing. The objective of this research was to estimate the shelf life of frozen cooked meat dumplings, based on the changes in the selected physico-chemical and sensory quality attributes. Precooked frozen meat dumplings sampled from the same lot produced at a frozen food company in Seoul were stored at -10, -15, and -20℃. The meat dumplings sampled at 3- to 4-week intervals were subjected to the determination of volatile basic nitrogen (VBN), thiobarbituric acid (TBA), peroxide value (POV), surface color and shear value after thawing at 5℃ for 15 h. High quality life (HQL) was determined through the triangle test and practical quality life (PQL) through the scoring test. VBN, TBA and POV increased as the storage period was increased. HQL of the frozen meat dumplings were 189 and 252 days at -10 and -15℃, respectively. The estimated PQL were 349, 475, and 491 days at -10, -15, and -20 ℃, respectively. Statistical analysis indicated that there were significant relationships between sensory scores and VBN and POV of the stored meat dumplings. VBN was selected as the most reliable objective quality index for the shelf life determination (PQL) of the precooked frozen meat dumplings.

Sodium butyrate has context-dependent actions on dipeptidyl peptidase-4 and other metabolic parameters

Lee, Eun-Sol,Lee, Dong-Sung,Pandeya, Prakash Raj,Kim, Youn-Chul,Kang, Dae-Gil,Lee, Ho-Sub,Oh, Byung-Chul,Lee, Dae Ho The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

Sodium butyrate (SB) has various metabolic actions. However, its effect on dipeptidyl peptidase 4 (DPP-4) needs to be studied further. We aimed to evaluate the metabolic actions of SB, considering its physiologically relevant concentration. We evaluated the effect of SB on regulation of DPP-4 and its other metabolic actions, both in vitro (HepG2 cells and mouse mesangial cells) and in vivo (high fat diet [HFD]-induced obese mice). Ten-week HFD-induced obese C57BL/6J mice were subjected to SB treatment by adding SB to HFD which was maintained for an additional 16 weeks. In HepG2 cells, SB suppressed DPP-4 activity and expression at sub-molar concentrations, whereas it increased DPP-4 activity at a concentration of $1,000{\mu}M$. In HFD-induced obese mice, SB decreased blood glucose, serum levels of insulin and $IL-1{\beta}$, and DPP-4 activity, and suppressed the increase in body weight. On the contrary, various tissues including liver, kidney, and peripheral blood cells showed variable responses of DPP-4 to SB. Especially in the kidney, although DPP-4 activity was decreased by SB in HFD-induced obese mice, it caused an increase in mRNA expression of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$. The pro-inflammatory actions of SB in the kidney of HFD-induced obese mice were recapitulated by cultured mesangial cell experiments, in which SB stimulated the secretion of several cytokines from cells. Our results showed that SB has differential actions according to its treatment dose and the type of cells and tissues. Thus, further studies are required to evaluate its therapeutic relevance in metabolic diseases including diabetes and obesity.

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>

High-throughput screening of protein variations in soybean seeds.

Vijayanand Velusamy,Kyung Jun Lee,Bo-Keun Ha,Jin Baek Kim,Sang Hoon Kim,Si-Yong Kang,Dong Sub Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The protein in soybean seeds accounts for approximately 40% of the dry seed weight. Two major storage proteins, 7S and 11S, constitute 70-80% of the total storage proteins in the seeds. In this study, the variation of total soluble protein extracts from 1152 soybean landraces that have been collected from South Korea were studied using high-throughput screening method with HT Protein Express Labchip (Caliper Life Sciences, Inc.). Seven distinct protein band patterns - four protein sub-units of 11S and three sub-units of 7S, were taken into account and their presence or absence were analyzed. Among the 1152 landraces, 525 genotypes were identified as lacking lipoxygenase, 255 lacking α1 subunit, 680 lacking α subunit, 169 lacking β subunit, 140 lacking acidic, 114 lacking Kunitz Trypsin Inhibitor (KTi) and 199 lacking basic protein patterns. The high-throughput protein analysis is helpful in screening a large number of populations with less time and minimum labor. The selected genotypes with low amounts or lacking of anti-nutritional factors such as trypsin inhibitor, lipoxygenase and α subunit would be used for future breeding purpose of quality improvement in soybean protein.

고온 폐가스 여과를 위한 집진기술

이동섭 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(A)

Hot gas media filtration for temperature above 300℃ and mainly up to 600℃ is an evolving technology becoming more and more popular in different industry segments. With ever tightening restrictions on discharge emission levels all process industries are having to review their present operations and incorporate means of reducing to a minimum heir effluent discharges Bench scale candle filters (60 O.D×10t×300L) were tested using different dusts collected from many industries including paper, glass and cement manufacturing plants. Collection efficiencies range 99.67% for below 1㎛ diameter and 99.9% for above 1㎛ diameter particles. Darcy's law coefficients range 0.5×10^11 to 2×10^11/㎡ depending on manufacturing conditions and composition of binding materials. Detachment and filtration of articles were performed effectively using 3bar pulsing pressure, 300msec pulse jet valve opening time, 5min filtration cycle, 1g/min particle loading and 10㎝/sec face velocity.

탄화규소 세라믹 필터를 이용한 여과기술에 관한 연구

이선하,이동섭 경운대학교 산업기술연구소 2000 産業技術硏究論文誌 Vol.3 No.1(B)

Candle type SiC filters applicable to hot gas filtration of IGCC and PFBC were manufactured and tested using two different dusts collected from cokes and water glass manufacturing processes. Particle sizes, porosities, and mean pore sizes of manufactured SiC filters range 150∼300㎛, ∼40%, and 43∼83㎛, respectively. Collection efficiencies of two different types of dusts rage 99.09∼99.95% at 600℃ and latm test condition. At the same condition, Darcy's resistance coefficients rage 1.149×10^10∼3.514×10^11m^-2 and differential pressures range 187∼391㎜H₂O. Also it was found that the smaller the mean pore size, the higher the differential pressure at the same operating conditions. SiC filters have high potential as a fine dust removal medium from HTHP processes like PFBC and IGCC. Furthermore, long-term performance tests of the filters are necessary for the commercialization of SiC filters.

저밀도 세라믹 필터의 특성 및 내구성에 관한 연구

이동섭,이선하,박진식 경운대학교 산업기술연구소 2001 産業技術硏究論文誌 Vol.3 No.2(B)

This study was Designed to examine the applicability of low density ceramic filters to actual processes through long term experiment to reveal the characteristics ceramic filter, durability against thermal and mechanical shocks. The experiment was also conducted at 600℃ and 300℃. 1m long low density ceramic filter with 60㎜ outer diameter and 10㎜ thickness was tested with dust collected from cokes process of P-steel. Collection efficiency ranged 99.98∼99.99% and conditioning was completed after 150∼200hrs. Resistance coefficients of filters range 5.9×10^10 at 600℃, 1.2×10^10 at 300℃. Long term experiments show that conditioning was completed after 155 hours of test at 300℃ and 165 hours at 600℃ with high collections efficiencies. Also tested filters show mechanical stabilities at different particulate feed rates ranging 0.5g/min to 1.0g/min. Results show that tested filters can be applied to commercial processes with one year guarantee.

자동 생성 웹 설문지를 이용한 팀 프로젝트 평가 시스템의 설계 및 구현

이미영,민수홍,조동섭 한국컴퓨터교육학회 2003 컴퓨터교육학회 논문지 Vol.6 No.4

우리나라 제7차 교육과정에서는 개별적 평가 외에 팀을 편성하여 팀 단위로 과제를 해결하도록 하는 집단적 평가 방법을 적용하여 문제를 해결하도록 유도하고 있다. 그러나 팀 단위의 협동적인 문제해결 과정을 통해 고등 사고력이나 사회적 기술 등을 기를 수 있도록 하는 팀 프로젝트에 대한 필요성은 증가하고 있는데 반해, 대다수의 교사들은 관리, 운영 등의 어려움을 이유로 팀 프로젝트 시행을 꺼려하는 경향이 있다. 따라서 본 논문에서는 팀 프로젝트 평가에 있어서의 현실적인 문제점을 인식하고, 교사의 팀 프로젝트 평가에 따른 부담을 덜어주면서 다면적인 평가를 통해 평가의 신뢰도와 타당도을 높이기 위해 자동 생성 웹 설문지를 이용한 팀 프로젝트 평가 시스템을 설계 및 구현하였다. The Seventh Curriculum uses not only individual assessment but also team assessment method that organizes and solves assignments by team unit. It also helps students to learn cooperation and responsibility and share the ideas and process of the team project with other students through class presentation. It is also cooperative teaming which is performed by several students together. Therefore, the assessment of team project is required to synthetically assess the performance process not the itself. This thesis will examine the actual problems about team project assessment and analyze related literatures. Then, this study designs and implements a practical system which alleviates the burden of teachers in charge of a team project assessment and improves reliability and validity in assessing team projects through multilateral assessment aiming at process as well as result objectives.

세리믹 필터를 이용한 고온에서 먼지제거에 대한 이용 기술

이동섭 경운대학교 산업기술연구소 2000 産業技術硏究論文誌 Vol.3 No.1(A)

Many European institutions and companies are developing ceramic filters which can substitute particulates and gas removal devices currently in use and which are believed to be the control devices of next generation. Because of their resistance to both chemical and thermal shocks, ceramic filters are an obvious choice for a high temperature filtration medium. Ceramic filter system alone can remove particulates and gaseous air pollutants simultaneously with out compulsory cooling systems, thus reducing installation and operating cost significantly. Emission standards of air pollutants are getting stringent and the market share of air pollutants control devices in Korea is projected up to 500 billion won by the year 2001 and high temperature low density ceramic filter system can substitute electrostatic precipitators and conventional bag filter systems significantly.