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        Selected microRNA-192 mutant indicates association with several function genes in bovine cells

        Chen Zi,Dexin Zeng,Jiyong Zhou,Jianjun Dai,Luyan Jiang,Feng Xue,Yuan Jiang,Baoguang Li 한국유전학회 2018 Genes & Genomics Vol.40 No.4

        MicroRNAs are implicated in many cellular processes such as cell differentiation and development, tumorigenesis, and immune regulation. In this study, miR192 was detected using quantitative real-time polymerase chain reaction (qRT-PCR) when MDBK cells were exposed to Escherichia coli. Cells with malfunction of bta-miR-192 were established using transcription activator-like effector nuclease (TALEN) technology. Finally, bta-miR-192 mutant cells were screened for differentially expressed genes using RNA-sequencing (RNA-seq). The results showed that miR192 significantly decreased in cells exposed to E. coli F18ac and E. coli K88ac. The RNA-seq results showed that 1673 differentially expressed transcripts were identified; 890 genes were upregulated and 775 genes were downregulated. With the gene ontology enrichment analysis, 431 differentially expressed genes (DEGs) were classified into 937 gene ontology terms. The pathway enrichment analysis showed that 535 genes were involved in 254 pathway terms. Interestingly, most of these DEGs were associated with the pathways in cancers or infectious diseases. When the selected DEGs (n = 162) in these pathways were intersected with 120 differential transcripts, 11 DEGs were identified. Subsequently, several genes associated with regulation, cancers, or viral infections, such as LEF1, AXIN2, MX1, and FCGR2B, were identified among the DEGs using functional analysis. Furthermore, associations between bta-miR-192 and DEGs were detected by intersecting the bta-miR-192’s target genes with the DEGs, indicating that three genes including CBL, DICER1 and TRERF1 were involved in this relationship. These findings provided useful guidance for investigating the role played by bta-miR-192 in cellular functionality in bovine cells.

      • Research of the Exciting Current Detection Method of MCR

        Wang Jun,Wei Liangliang,Chen Feng,Nie Dexin,Lu Wenhua 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.5

        Magnetic valve controllable reactor (MCR) is a kind of dynamic reactive power compensation device. The exciting current of MCR is one of the important indexes of operating condition of MCR. This paper firstly analyzed the principle of MCR, and deduced the relationship between the exciting current and the output current, verified by MATLAB/SIMULINK. And then an effective method to detect the exciting current was proposed by this paper. The method has the advantages of optical fiber isolation, high detection precision, strong anti-interference, etc. Finally, a 220v/1200var MCR was experimented in detail. The results demonstrate the accuracy of the theoretical analysis.

      • KCI등재

        Hyphae and organic acids of Aspergillus Niger promote uranium recovery by destroying the ore surface and increasing the porosity and permeability of ores

        Wang Yongdong,Wang Jinhua,Ding Dexin,Li Guangyue,Sun Jing,Hu Nan,Li Feng,Ma Jianhong,Zhang Hui,Ding Yang,Dai Zhongran 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.5

        To elucidate the mechanism of damage caused by hyphae and organic acids produced by Aspergillus Niger on the surface and internal structure of uranium ore, direct uranium leaching, indirect uranium leaching and semidirect uranium leaching were conducted, and the surface morphology, strength, mineral crystallinity, porosity, and permeability of the ore were analyzed. The results demonstrated that the combination of biomechanical forces exerted by hyphae and the complexation effects of organic acids led to the dissolution of SiO2 and other substances on the surface of ore, resulting in exfoliation from the exterior to the interior, thereby promoting uranium recovery. Furthermore, the proton exchange involving H+ and the complexation of organic acids resulted in the dissolution of cations within the ore, causing destruction to the crystal lattice structure of minerals and increasing the porosity and permeability inside the ore. The dominant factor contributing to ore damage during recovery was organic acids.

      • KCI등재

        Improving the Microbial Production of Amino Acids: From Conventional Approaches to Recent Trends

        Guoqing Zhang,Xueni Ren,Xiuhong Liang,Yaqun Wang,Dexin Feng,Yujun Zhang,Mo Xian,Huibin Zou 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.5

        Variable industrial strains have been applied in the fermentation of bulk amino acids. Strain discovery and evolution, process optimization are traditional approaches to improve the yield and efficiency of the bio-production process, hence to compete with chemical or enzymatic process in amino acids production. With the fast development of bioengineering and synthetic biology, the strains can be rationally engineered to achieve better performance and gain the capacity in the fermentation of broader range of amino acids, especially for value-added amino acids. This proposed review aims to summarize traditional and recent strains in the microbial production of amino acids, characterize their metabolic pathways and present potential objectives for rational evolution. In addition, this proposed review prospect the recent opportunities and challenges in the microbial production of value-added amino acids (rare amino acids, non-canonical amino acids, and unnatural amino acids).

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