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Chemical biology-based approaches on fluorescent labeling of proteins in live cells
Jung, Deokho,Min, Kyoungmi,Jung, Juyeon,Jang, Wonhee,Kwon, Youngeun The Royal Society of Chemistry 2013 Molecular bioSystems Vol.9 No.5
<P>Recently, significant advances have been made in live cell imaging owing to the rapid development of selective labeling of proteins <I>in vivo</I>. Green fluorescent protein (GFP) was the first example of fluorescent reporters genetically introduced to protein of interest (POI). While GFP and various types of engineered fluorescent proteins (FPs) have been actively used for live cell imaging for many years, the size and the limited windows of fluorescent spectra of GFP and its variants set limits on possible applications. In order to complement FP-based labeling methods, alternative approaches that allow incorporation of synthetic fluorescent probes to target POIs were developed. Synthetic fluorescent probes are smaller than fluorescent proteins, often have improved photochemical properties, and offer a larger variety of colors. These synthetic probes can be introduced to POIs selectively by numerous approaches that can be largely categorized into chemical recognition-based labeling, which utilizes metal-chelating peptide tags and fluorophore-carrying metal complexes, and biological recognition-based labeling, such as (1) specific non-covalent binding between an enzyme tag and its fluorophore-carrying substrate, (2) self-modification of protein tags using substrate variants conjugated to fluorophores, (3) enzymatic reaction to generate a covalent binding between a small molecule substrate and a peptide tag, and (4) split-intein-based C-terminal labeling of target proteins. The chemical recognition-based labeling reaction often suffers from compromised selectivity of metal–ligand interaction in the cytosolic environment, consequently producing high background signals. Use of protein–substrate interactions or enzyme-mediated reactions generally shows improved specificity but each method has its limitations. Some examples are the presence of large linker protein, restriction on the choice of introducible probes due to the substrate specificity of enzymes, and competitive reaction mediated by an endogenous analogue of the introduced protein tag. These limitations have been addressed, in part, by the split-intein-based labeling approach, which introduces fluorescent probes with a minimal size (∼4 amino acids) peptide tag. In this review, the advantages and the limitations of each labeling method are discussed.</P> <P>Graphic Abstract</P><P>This review summarizes various approaches to incorporate synthetic fluorescent probes to target proteins in live cells as well as current efforts to control the fluorescent signal using external stimuli. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2mb25422k'> </P>
Photo-triggered fluorescent labelling of recombinant proteins in live cells
Jung, Deokho,Sato, Kohei,Min, Kyoungmi,Shigenaga, Akira,Jung, Juyeon,Otaka, Akira,Kwon, Youngeun The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.47
<P>A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.</P> <P>Graphic Abstract</P><P>A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed for background-free fluorescent labelling of target proteins with the necessary spatiotemporal control. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc01067e'> </P>
Laser CVD Repair Source 특성 비교에 대한 연구
정성원(Sungwon Jung),정덕호(Deokho Jung),노순준(soonjonn rho) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
Liquid crystal display device is a Display Industry demand evolve, and also has increased the size of a glass substrate. The size of the glass substrate increases, price will go up too. And fine pattern on a glass substrate configuration as the production costs of Liquid crystal display device will go up further. So the end of production of fine patterns of a liquid crystal display device malfunction occurs in some cases lose their commercial value. Away valuable products, by modifying the circuit to restore if you can help improve productivity. How to restore the fine pattern of Liquid crystal display device using Laser CVD Repair is the way. In this case, the Source to the Mo, W is used, what material is suitable as the Source is a comparative study of characteristics.