http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Deok Ho Kwon,Chae Eun Hwang,Jae Young Choi,Yeon Ho Je,Si Hyeock Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
Western blot analysis using acetylcholinesterase (AChE)-specific antibody was conducted to determine whether AChE gene (Tuace) duplication actually results in overproduction of AChE in Tetranychus urticae (TuAChE). The protein quantities of TuAChE in seven field-collected mite populations were precisely correlated with the copy numbers. To investigate the effects of each mutation on AChE insensitivity and possible fitness cost, eight variants of TuAChE were in vitro expressed using the baculovirus expression system. Kinetic analysis revealed that the Ala391Thr mutation did not change kinetic properties of AChE, whereas the Gly228Ser and Phe439Trp mutations significantly increased the insensitivity to monocrotophos. Moreover, when the Gly228Ser and Phe439Trp mutations are present together, insensitivity increased over a thousand-fold, showing that both mutations confer resistance in a synergistic manner. Presence of the mutations, however, reduced catalytic efficiency of AChE considerably, suggesting an apparent fitness cost in monocrotophosresistant mites. Reconstitution of the multiple copies of AChE having different compositions of mutations revealed that the catalytic efficiencies of the six-copy and two-copy AChEs (resembling the AD and PyriF strains of mite, respectively) were still lower but comparable to that of wildtype AChE. These finding clearly suggested that multiple rounds of Tuace duplication was needed to compensate the reduced catalytic activity of AChE caused by mutations.
Molecular characterization of porcine <i>PGM1</i> gene associated with meat quality traits
Hwang, Jung Hye,Kwon, Seul Gi,Park, Da Hye,Kim, Tae Wan,Kang, Deok Gyeong,Ha, Jeongim,Kim, Sam Woong,Kim, Chul Wook Canadian Science Publishing 2015 Canadian journal of animal science Vol.95 No.1
<P> Hwang, J. H., Kwon, S. G., Park, D. H., Kim, T. W., Kang, D. G., Ha, J., Kim, S. W. and Kim, C. W. 2015. Molecular characterization of porcine PGM1 gene associated with meat quality traits. Can. J. Anim. Sci. 95: 31-36. The PGM1 gene from four porcine breeds (Berkshire, Duroc, Landrace, and Yorkshire) is highly expressed in liver tissue at the transcriptional level. Single nucleotide polymorphisms (SNPs) of PGM1 were examined to analyze association with increased expression of PGM1 gene in the Berkshire liver. A Leu525 synonymous SNP of Chr6:137174682A@@>@@G (c.1575A@@>@@G) was identified and showed significant (P@@<@@0.05) differences to backfat thickness, drip loss, protein content, fat content, Warner-Bratzler shear force, and post-mortem pH24h. Therefore, it is concluded that PGM1 synonymous SNP is an important factor regulating meat quality. </P>
Atomic structure of conducting nanofilaments in TiO2 resistive switching memory
Kwon, Deok-Hwang,Kim, Kyung Min,Jang, Jae Hyuck,Jeon, Jong Myeong,Lee, Min Hwan,Kim, Gun Hwan,Li, Xiang-Shu,Park, Gyeong-Su,Lee, Bora,Han, Seungwu,Kim, Miyoung,Hwang, Cheol Seong Springer Science and Business Media LLC 2010 Nature nanotechnology Vol.5 No.2
Chae Eun Hwang,Keon Mook Seong,Deok Ho Kwon,Young Ho Kim,Jae Young Choi,Yeon Ho Je,Si Hyeock Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
We analyzed molecular and enzymatic properties of three cholinesterases (ChEs; ClAChE1, ClAChE2 and ClSChE) from Cimex lectularius. The ClAChE1 and ClAChE2 were generally present as a membrane-anchored dimeric insoluble form in the brain and ganglia. In the case of ClSChE, monomeric and dimeric soluble forms were observed. To investigate enzymatic properties, three ChEs were functionally expressed using baculovirus expression system. ClAChE1 revealed a significantly higher activity than ClAChE2 to acetylthiocholine iodide (ATChI) substrate. Kinetic analysis using two choline substrates (ATChI and butyrylthiocholine iodide) demonstrated that ClAChE2 had higher catalytic efficiency but lower substrate specificity than ClAChE1. Inhibition assay was conducted by using three inhibitors (BW284C51, eserine, Iso-OMPA) and two insecticides (chlorpyrifos-methyl and carbaryl). Two ClAChEs revealed high sensitivities to BW284C51, eserine, chlorpyrifos-methyl and carbaryl, but were not sensitive to Iso-OMPA. This inhibition profile confirmed that both ClAChEs are categorized as ChEs. Interestingly, the salivary specific cholinesterase did not show any measurable activities to choline substrates, confirming its non-synaptic function in C. lectularius
Kwon, Seul Gi,Hwang, Jung Hye,Park, Da Hye,Kim, Tae Wan,Kang, Deok Gyeong,Kang, Kyung Hee,Kim, Il-Suk,Ha, Jeongim,Kim, Chul Wook,Plaizier, J. Canadian Science Publishing 2016 Canadian journal of animal science Vol.96 No.1
<P> Corticosteroid-binding globulin (CBG) is a plasma transport protein that has glucocorticoid-binding activity. In the present study, we identified CBG gene expression in several tissues of four pig breeds: Berkshire, Duroc, Landrace, and Yorkshire. Expression of CBG mRNA was detected in the liver of all four breeds, and was the highest in Berkshire pigs. We also found single nucleotide polymorphisms (SNPs) in the CBG gene from Berkshire pigs, including SNP c.919G@@>@@A, which corresponds to 307G@@>@@R. We analyzed the relationships between this CBG variant and various meat-quality traits. The SNP was significantly associated with backfat thickness, post-mortem pH24h, meat color [CIE a* (redness), CIE b* (yellowness)], water-holding capacity, fat content, moisture content, protein content, cooking loss, and shear force. However, the effects differed by gender: the values were significantly associated with almost all traits in gilts, whereas only cooking loss and shear force were shown significantly in barrows. The variant G allele was associated with decreases in backfat thickness, post-mortem pH24h, CIE a*, fat content, and cooking loss, but with increases in CIE b*, water-holding capacity, moisture content, protein content, and shear force. Because the general correlation between meat-quality traits were not validated in the present study, we suggest that certain SNP might be used in the restrictive application to distinguish meat-quality traits. </P>
A single port surgical robot system with novel elbow joint mechanism for high force transmission
Hwang, Minho,Yang, Un-Je,Kong, Deokyoo,Chung, Deok Gyoon,Lim, June-gi,Lee, Dong-Ho,Kim, Daniel H.,Shin, Dongsuk,Jang, Taeho,Kim, Jeong-Whun,Kwon, Dong-Soo Wiley (John WileySons) 2017 INTERNATIONAL JOURNAL OF MEDICAL ROBOTICS AND COMP Vol.13 No.4
Kwon, Seulgi,An, Sang Mi,Yu, Go Eun,Hwang, Jung Hye,Park, Da Hye,Kang, Deok Gyeong,Kim, Tae Wan,Park, Hwa Chun,Ha, Jeongim,Kim, Chul Wook,Plaizier, J. Canadian Science Publishing 2018 Canadian journal of animal science Vol.98 No.4
<P> Litter size is an important trait in the pig industry. Therefore, a lot of effort has been put into improving this trait. DNA methylation is an essential epigenetic modification present in unique DNA sequences. Alterations in methylation can affect transcription and phenotypic variation. The purpose of this study was to investigate the effect of DNA methylation on litter size. Methylation-specific restriction enzymes are simple and useful tools for detecting DNA methylation status. We used a pair of methylation-sensitive isoschizomers, which have the same recognition site, HpaII and MspI. Insulin-like growth factor binding protein 4 (IGFBP4) is a key regulator of ovarian follicular development and fetal growth in eutherian mammals. In this study, we discovered that IGFBP4 was hyper-methylated in the uterus tissue of a larger litter size group using bisulfite sequencing, and validated the positive relationship between the methylation status of IGFBP4 and the total number born of pigs using the porcine methylation-specific restriction enzyme polymerase chain reaction (PMP) assay. We suggest that the IGFPB4 gene can be used as a prognostic biomarker for hyperprolific sows and that the PMP assay is a useful tool for methylation status screening. </P>
Pathogenicity and Single Dose Toxicity of a Potential Probiotic Lactobacillus spp. PSC101 in Mice
Hwang, Mi-Hyun,Kim, Young-Hwon,Kim, Eun-Young,Song, Jae-Chan,Lee, Keun-Woo,Jeong, Kyu-Shik,Kim, Kil-Soo,Rhee, Man-hee,Kwon, Oh-Deok Korean Society of ToxicologyKorea Environmental Mu 2004 Toxicological Research Vol.20 No.2
This study was conducted to investigate the pathogenicity and acute single toxicity of Lactobacillus spp. PSC101 (PSC101) isolated from pigs and L. acidophilus (LA) at 2.5$\times$$10^9$CFU or 2.5$\times$$10^{12}$colony forming units (CFU) in mice for 14 days. After oral administration of the bacteria into mice, we could not find their any specific pathogenicity from the standpoints of clinical signs, and changes in body weight and body temperature, as compared with the control group during 14 days. We further investigated the toxicity of concentrated culture broth ($\times$10) after fermentation of them for safe industrial process. As the results, we could not find any clinical signs, changes in body weight and body temperature, as compared with the control group (MRS broth) for 14 days. The results obtained in this study suggest that the potentially probiotic, PSC101, is non-toxic in mice and is therefore likely to be safe for pig use.
Hwang, Seon-Hwan,Kwon, Soon-Kurl,Hwang, Young-Gi,Bang, Deok-Je The Korean Institute of IIIuminating and Electrica 2014 조명·전기설비학회논문지 Vol.28 No.12
This paper proposes a coordinated control for an independent multi-phase transverse flux linear synchronous motor (IM-TFLSM) based on magnetic levitation. The stator structures of the IM-TFLSM are composed of a two set, which has independent three-phase windings and a double-sided air-gap as opposed to the conventional Y-connected three-phase linear motors. A suitable control algorithm is necessary to operate the applied linear machine. This study proposes a coordinated control algorithm for adjusting the mover air-gap and thrust force of the IM-TFLSM in order to maintain air-gap and phase shifted current control of the independent 3-phase modules. In addition, the principle of operation and its special structures are described in detail and the validity and effectiveness of the control algorithm is verified through multiple experimental results.