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MTB-DR-RIF 9G membrane: a platform for multiplex SNP detection of multidrug-resistant TB.
Sayyed, Danishmalik Rafiq,Nimse, Satish Balasaheb,Song, Keum-Soo,Sung, Nackmoon,Kim, Taisun Springer-Verlag 2015 Analytical and Bioanalytical Chemistry Vol.407 No.19
<P>The MTB-DR-RIF 9G membrane can detect by detecting multiple mutations in multiple codons. The MTB-DR-RIF 9G membrane possesses clinical applicability in point-of-care settings for the following reasons: (i) 100% similar results with that of the sequencing analysis for clinical samples, (ii) discrimination of the multiple mutations in multiple codons, (iii) a specific/non-specific hybridization ratio higher than 350:1, and (iv) the sensitivity was found to be 1-10 copies/test for detection and discrimination of the wild and mutant TB strains. Graphical abstract Schematic illustration of the effect of controller DNA on the hybridization of the immobilized probes (corresponding to the wild TB strain) with the PCR product of (a) wild TB strain and (b) mutant TB strain.</P>
Gene therapy using plasmid DNA-encoded anti-HER2 antibody for cancers that overexpress HER2
Kim, H,Danishmalik, S N,Hwang, H,Sin, J-I,Oh, J,Cho, Y,Lee, H,Jeong, M,Kim, S-H,Hong, H J Nature Publishing Group 2016 Cancer gene therapy Vol. No.
<P>Plasmid DNA-encoded antibodies, or DNA-based monoclonal antibodies (dMAbs), are delivered by intramuscular injection and <I>in vivo</I> electroporation (EP) and are effective in virus neutralization, although they have not been evaluated for tumor gene therapy. Here we investigated whether a dMAb was appropriate for tumor gene therapy. We constructed the expression plasmids coding for the heavy or light chain of a parental murine antibody of Herceptin with the antibody genes codon- and RNA-optimized and fused to the Kozak-IgE leader sequence in pVax1. Transfection of the plasmids into human muscle RD cells resulted in functional expression of the antibody, and this exhibited the same <I>in vitro</I> antiproliferative activity as Herceptin. A single intramuscular injection and <I>in vivo</I> EP of the plasmids (100 μg per head) resulted in high and sustained antibody expression in the sera of normal mice and in effective inhibition of tumor growth in nude mice bearing HER2-positive human breast carcinoma BT474 xenografts. The antitumor efficacy of the anti-HER2 dMAb was similar to that of four doses of intravenously injected 10 mg kg<SUP>−1</SUP> Herceptin. The results demonstrate that the dMAb is effective in the treatment of HER2-positive breast cancer, suggesting that this dMAb may be applicable for tumor gene therapy.</P>
A new platform for a convenient genotyping system
Song, Keum-Soo,Nimse, Satish Balasaheb,Kim, Junghoon,Sayyed, Danishmalik Rafiq,Kim, Taisun The Royal Society of Chemistry 2013 Chemical communications Vol.49 No.26
<P>The high SNP discrimination ratio of 360 : 1, 100% target-specific hybridization at 25 °C, detection limit of 10<SUP>1</SUP> copies, and differentiation of 10<SUP>1</SUP> to 10<SUP>7</SUP> copies of the PCR product of high-risk HPV genotypes in clinical samples ensure the application of the 9G membrane in a convenient platform for DNA genotyping.</P> <P>Graphic Abstract</P><P>The high SNP discrimination ratio of 360 : 1, 100% target-specific hybridization at 25 °C, detection limit of 10<SUP>1</SUP> copies, and differentiation of 10<SUP>1</SUP> to 10<SUP>7</SUP> copies of the PCR product of high-risk HPV genotypes in clinical samples ensure the application of the 9G membrane in the point-of-care DNA genotyping. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3cc39231g'> </P>
Nimse, Satish Balasaheb,Song, Keum-Soo,Kim, Junghoon,Sayyed, Danishmalik Rafiq,Kim, Taisun Molecular Diversity Preservation International (MD 2013 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.14 No.3
<P>A 9G DNAChip obtained by allowing the formation of a self-assembled monolayer (SAM) of oligonucleotides appended with nine consecutive guanines on the chip surface has been applied in the detection of biomarkers. Using a 9G DNAChip, biomarker in the concentration range of 4 pg/mL to 40 fg/mL can be easily differentiated in the buffer matrix. Moreover, it is the first time that a biomarker with a concentration of 40 fg/mL has been detected in a mixture of proteins without use of any signal amplification technique.</P>