개발제한구역의 실태와 개선방향에 관한 연구

강대욱 慶南專門大學 1996 論文集 Vol.24 No.1

재건축에 관한 거주민 의식의 실태연구

강대욱,김혜민 慶南專門大學 1995 論文集 Vol.23 No.1

大單位 住居團地의 規模計劃的 特性에 관한 硏究 : 釜山市 宅地開發事業地를 中心으로 Focus on the Housing Lot Development Projects in Pusan

姜大旭 釜山大學校 都市問題硏究所 1997 都市硏究報 Vol.5 No.-

The purpose of this paper is to find out the characteristics of pattern in large scale residential districts. For the above purpose, literature and field survey was carried out with the large scale residential district projects which were executed in Pusan. The main results obtained from the study were as follows. 1. The pattern of development project is affected by the conditions of location and easiness of execution. 2. The developing circumstance of peripheral district is good prepared, the ratio of housing lot is shown upward tendency. 3. The density of residential district is increased by the change of times.

전단벽식 아파트에서 슬래브의 역학적 거동에 관한 연구

김혜민,강대욱 慶南專門大學 1995 論文集 Vol.23 No.1

釜山市 都心部의 範圍設定과 空間構造에 관한 硏究

孫泰敏,姜大旭 釜山大學校 都市問題硏究所 1988 都市硏究報 Vol.2 No.-

We examined the volume and floor area of buildings and their uses to analyse the internal structure of the central area in Pusan. In this paper, the floor use of central function was utilized for the central area delimitation, and the boundary of central area in Pusan is connected with blocks central business height index(CBHI)≥1.5 and central business intensity index (CBII)≥50. The results of this are as follows : 1. Main central function in Pusan's central area is identified as management, commercial and service activity. 2. If the central area is identified by the blocks which have high concentration of central function, the boundary of the central area is generally delimited around park Yong-doo san, Nam-po and Kwang-bok dong. 3. Approximately 1,794 buildings are located in the Pusan's central area. The proportion of lower building(5 and under) is as much high as 96.8% of the total number of buildings in central area. The total floor area of buildings in the Pusan's central area is about 4.1 million M^2 and it comes to be as much 3.42 times of the total ground floor. 4. The percentage of the floor area for business management activity is 21.6% and it is the highest among others. Beverage-eats service which has 15.5% in the percentage comes next. Amusement-cultural service which has 8.5% takes the third position, and so on… 5. In order to analyse the locational specialization of activities, the central area is devided 8 districts. And the method of location quotient index(L. Q) is adopted each district. L. Q of each district is expressed various form because of the advantage of agglomeration economy.

방선균 분리주가 생산하는 Phospholipase C 저해물질인 MT2617-2B의 분리 및 특성

고학룡,이현선,오원근,안순철,김보연,강대욱,민태익,안종석 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

방선균 분리주 MT2617-2의 배양액으로 부터 phospholipase C (PLC) 저해물질인 MT2617-2B를 n-butanol 추출 및 column chromatography 법을 이용하여 분리하였다. MT2617-2B는 IR ^13C- 및 ^1H-NMR 그리고 ESI-MS에 의한 구조분석 결과, 한 개의 hemiketal ring, polyhydroxyl 및polymethyl groups으로 구성되었으며 side chain으로 한 개씩의 malonate 및 guanidine group을 가지는 분자량 1057의 macrolide 화합물이었다. 따라서, MT2617-2B를 기존의 macrolide 항생제인 copiamycin 및 niphithricin A로 동정하였다. 한편, MT2617-2B는 methanol 용액에서 실온에서 방치하였을 때 도일한 분자량을 가진 두 개의 이성질체를 생성하였다. PLC γ1과 -β1에 대해 각각 25 및 50㎍/㎖의 IC_50 값을 가지며, Staphylococcus aureus 와 Candida albicans에 대해서는 항균활성을 나타내지만 Escherichia coli에는 나타내지 않았다. A phospholipase C (PLC) inhibitor (MT2617-2B) was isolated from the culture broth of actionmycetes isolate MT2617-2 by the extraction with n-butanol and column chromatographic techniques. The molecular weight of the inhibitor was 1057, by the spectroscopic analyses of IR ^13C- and ^1H-NMR and ESI-MS. The chemical structure of MT2617-2B was found to be a macrolide compound consisted of a hemiketal ring, polyhydroxyl and polymethyl groups, which had a malonate and guanidine group as its side chain. MT2617-2B produced its two isomers having the same molecular weight by standing in methanol solution at room temperature. Therefore, MT2617-2B was identified as copiamycin and niphithricin A, macrolide antibiotics. The values of IC_50 against PLC γ1 and PLC-β1 were 25 and 50㎍/㎖, respectively. MT2617-2B had antimicrobial activities against Staphylococcus aureus and Candida albicans, but not against Escherichia coli.

Production and Rheological Properties of Bioflocculant Produced by Bacillus sp. DP-152

( DAE OOK KANG ),( HEE MOCK OH ),( Hyun Hyo Suh ),( Byung Dae Yoon ),( HEE SIK KIM ),( SEONG HOON MOON ),( Hyoung Kab Kim ),( Gee Ill Jun ),( Hyun Seoun Park ) 한국미생물생명공학회 1998 Journal of microbiology and biotechnology Vol.8 No.6

제지폐수 처리용 미생물의 분리 및 복합 미생물제제의 개발

Dae Ook Kang(강대욱),Hyun-Hyo Suh(서현효) 한국생명과학회 2011 생명과학회지 Vol.21 No.4

제지폐수의 효율적인 생물학적 처리와 폐수특성에 적합한 미생물제제의 개발을 위하여 토양 및 산업폐수로부터 방향족 화합물에 분해활성이 높은 KN11, KN13 및 KN27 균주와 세포 외 섬유소 가수분해효소 생산 균주 GT21 등의 균주를 분리하였다. 형태학적, 생리학적 및 생화학적 분류를 통해 이들 분리주 KN11, KN13, KN27 및 GT21 등은 Acinetobactersp., Neisseriasp., Bacillussp., Pseudomonassp.와 유사한 것으로 판명되어 최종적으로 각각 Acinetobactersp. KN11, Neisseriasp. KN13, Bacillussp. KN27, Pseudomonassp. GT21로 명명하였다. 제지폐수 중 난분해성 물질과 COD 증가원인 물질을 분석하고자 GC/MS를 이용하여 방향족 화합물 및 그 유도체들을 검출하였다. 분리균주 Acinetobactersp. KN11, Neisseriasp. KN13, Bacillussp. KN27 및 Pseudomonassp. GT21의 균체로 구성된 미생물제제 J30을 제조하여 제지폐수의 효율적 처리를 위한 연구에 사용하였다. 미생물제제 J30의 제지폐수에서 COD 제거를 위한 최적온도와 pH는 각각 30℃와 7.5였으며 배양 60시간에서 최대의 COD 제거효율을 나타내었다. 실험실 규모의 pilot plant에서 미생물제제 J30의 COD 제거효율은 87%의 높은 제거효율을 나타내었다. This study was performed to investigate the effects of microbial augmentation on the biological treatment of paper mill wastewater. Three bacteria (KN11, KN13, KN27) capable of degrading aromatic compounds and a bacterial strain (GT21) producing an extracellular cellulase were isolated from soil and wastewater by selective enrichment culture. Through morphological, physiological, and biochemical taxonomies, isolated strains of KN11, KN13, KN27, and GT21 were identified as Acinetobactersp., Neisseriasp., Bacillussp., and Pseudomonassp. and named Acinetobactersp. KN11, Neisseriasp. KN13, Bacillussp. KN27, and Pseudomonassp. GT21, respectively. For analysis of non-biodegradable and chemical oxygen demand (COD)-increasing matter in a paper mill wastewater, we utilized GC/MS to detect aromatic compounds and their derivatives containing several substituted functional groups. The microbial augmentation, J30 formulated with the mixture of bacteria including Acinetobactersp. KN11, Neisseriasp. KN13, Bacillussp. KN27, and Pseudomonassp. GT21, was used for the treatment of paper mill wastewater. The optimum temperature and pH for COD removal of the microbial augmentation, J30, were 30℃ and 7.5, respectively. For evaluation of the industrial applicability of the microbial augmentation, J30 in the pilot test, treatment efficiency was examined using paper mill wastewater. The microbial augmentation, J30, showed a COD removal rate of 87%. On the basis of the above results, we designed the wastewater treatment process of the activated sludge system.

Molecular Cloning and Analysis of Sporulation-Specific Glucoamylase (SGA) Gene of Saccharomyces diastaticus

Kang, Dae-Ook,Hwang, In-Kyu,Oh, Won-Keun,Lee, Hyun-Sun,Ahn, Soon-Cheol,Kim, Bo-Yeon,Mheen, Tae-Ick,Ahn, Jong-Seog The Microbiological Society of Korea 1999 The journal of microbiology Vol.37 No.1

Sporulation-specific glucoamylase (SGA) gene was isolated from genomic library of Saccharomyces diastaticus 5114-9A by using glucoamylase non-producing mutant of S. diastaticus as a recipient. When the glucoamylase activities of culture supernatant, periplasmic, and intracellular fraction of cells transformed with hybrid plasmid containing SGA gene were measured, the highest activity was detected in culture supernatant. SGA produced by transformant and extracellular glucoamylase produced by S. diastaticus 5114-9A differed in enzyme characteristics such as optimum temperature, thermostability, and resistance to SDS and urea. But the characteristics of SGA produced by sporulating yeast cells and vegetatively growing transformants were identical.

자이모그라피 기술의 문제점과 해결

강대욱(Dae-Ook Kang),최낙식(Nack-Shick Choi) 한국생명과학회 2019 생명과학회지 Vol.29 No.12

효소는 세제, 식품, 사료, 의약품 및 의료용 분야 등 산업 전반적인 응용 분야에서 널리 사용되고 있으며, 산업제품 및 공정에서 주요 요인이다. 효소를 선별, 확인, 및 특성 분석을 위해 zymography 기술이 일상적으로 사용됩니다. Zymography 기술은 SDS-전기영동을 통해 단백질을 분리한 후 포함된 기질을 겔 상에서 분해하는 기능성 효소를 검출하는 데 널리 사용되는 단순하고 민감하며 정량화가 가능한 기술이다. 이 방법은 비 환원 조건하에서 SDS-전기영동 겔에서 전기영동에 의한 단백질의 분리와 겔 상에서 효소 활성을 검출하는 다목적 2 단계의 기술이다. 이는 SDS-전기영동 겔에 기질을 중합시키고 전기영동 분리 후 효소 반응 완충용액에서 복원된 가수분해 효소에 의해 분해되는 것을 기반으로 하는 기술이다. 미생물 배양액, 식물 추출물, 혈액, 조직 배양액, 식품 속 효소 및 메타 프로테옴을 포함한 어떤 종류의 생물학적 시료들을 zymography에 적용하고 분석이 가능하다. Zymography의 장점은 전처리 없이 혼합된 시료를 적용하여 SDS-전기영동 겔 상에서 활성을 지닌 단백질을 직접육안으로 검출이 가능할 뿐만 아니라 나노그람(nanogram) 수준에서 활성을 확인이 가능하다. 그래서 이 zymography 기술은 다양한 분야에 응용이 가능하다. 하지만, 이러한 장점이 오히려 단점으로 작용하여 실험적 오류를 범할 수 있는 경우가 많다. 본 총설에서 zymography 기술의 장점, 단점, 및 문제점 해결에 관해서 서술하였다. Enzymes are widely used in industrial applications such as detergents, food, feed production, pharmaceuticals and medical applications and major contributors to clean industrial products and processes. To screen, identify, and characterize the enzymes the zymography techniques are routinely used. The zymography technique is a simple, sensitive, and quantifiable technique that is widely used to detect functional enzymes following electrophoretic separation in sodium dodecyl sulfate (SDS)-polyacrylamide gels. The method is a versatile two-stage technique involving protein separation by electrophoresis followed by the detection of enzyme activity in polyacrylamide gels under non-reducing conditions. It is based on SDS-polyacrylamide gel (PAG) copolymerization with substrates, which are degraded by the hydrolytic enzymes restored in enzyme reaction buffer after the electrophoretic separation. Any kind of biological sample can be applied and analyzed on zymography, including culture supernatants of microbes, plants extracts, blood, tissue culture fluids, enzymes in foods extracts and metaproteome. The advantage of zymography is that it is possible to directly detect the protein with activity on the electrophoretic gel as well as confirm the activity at the nanogram level. Thus, this zymography technology can be applied in various fields. However, these advantages are rather disadvantageous and can often lead to experimental errors. In this review, the advantages, disadvantages, and problem solving of zymography technique are described.