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- 저자 : Cuixia Zhang (검색결과 3 건)
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Dandan Zhang,Cuixia Gao,Ruyi Li,Lin Zhang,Jingkui Tian 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.5
2a,3a,24-Thrihydroxyurs-12-en-28-oicacid (TEOA),a pentacyclic triterpenoid, isolated from the roots of Actinidiaeriantha, exhibits significant cytotoxicity against SW620, BGC-823, HepG-2, A549 and PC-3 cancer cells. In this study, weinvestigated the underlying molecular mechanism of the anticanceractivity of TEOA in SW620 cells.We demonstrated thatTEOA induced apoptosis through cleavage of caspase-9 andPARP in SW620 cells. In addition, evidence of TEOA-mediatedautophagy included the induction of autophagolysosomesand activation of autophagic markers LC-3B and p62. Furtheranalysis illustrated that TEOA promoted the phosphorylation ofPERK and elF2a, followed by up-regulation of the downstreamprotein CHOP, suggesting the involvement of PERK/eIF2a/CHOP pathway and ER stress in TEOA-induced autophagy inSW620 cells. Meanwhile, TEOA-mediated PINK1, Parkin,ubiquitin and p62 activation revealed that TEOA inducedspecific autophagy-mitophagy in SW620 cells. Additionally, anantioxidant NAC attenuated the TEOA-induced mitophagy,indicating that TEOA triggers mitophagy via a ROS-dependentpathway. Collectively, our findings revealed a novel cellularmechanism of TEOA in the colon cancer cell line SW620, thusproviding a molecular basis for developing TEOA into an antitumorcandidate.
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LAMP, PCR, and real-time PCR detection of Acetobacter aceti in yogurt
Wei Zhou,Yan Zhang,Shuang Wang,Yuehua Li,Jingjing Zhang,Cuixia Zhang,Zan Wang,Zhisheng Zhang 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.1
Acetic acid bacteria (AAB) can spoil food. Acetobacter aceti as a core subgroup of AAB is usually isolated from yogurt. A. aceti should be timely and effectively detected to prevent yogurt contamination. The present study focused on A. aceti to establish an assay that can be performed to detect AAB in yogurt. LAMP, PCR, and real-time PCR were applied and compared for detecting A. aceti from pure culture and artificially contaminated yogurt samples. In pure culture, LAMP showed the highest detection sensitivity with 10−1 CFU/mL. For yogurt samples, the sensitivity limit of LAMP was 102 CFU/mL, which was lower than that of real-time PCR (101 CFU/mL). The results indicated that these methods could be quickly and efficiently applied to detect A. aceti. As LAMP technology has low cost and high detection efficiency, it can potentially be applied for detecting A. aceti in production and quality control programs of yogurt.
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Jin’e Wan,Jian Yang,Cuixia Qiao,Xiaomei Sun,Aiting Di,Lize Zhang,Dandan Wang,Gang Zhao 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.5
Purpose: Colorectal cancer (CRC) is the third most common cancer in China and poses high morbidity and mortality. In recentyears, increasing evidence has indicated that microRNAs played important functions in the occurrence and development of tumors. The purpose of this study was to identify the biological mechanisms of miR-362 in CRC. Materials and Methods: Quantitative real-time PCR was carried out to assess the expression of miR-362 and SIX1. The Kaplan-Meier method was employed to evaluate the 5-year overall survival of CRC patients. The proliferative and invasive abilities of CRCcells were assessed by MTT and transwell assays. Results: miR-362 was significantly decreased in CRC tissues and cell lines, compared to the normal tissues and normal cells. Asignificant connection was confirmed between the overall survival of 53 CRC patients and low expression of miR-362. Downregulationof miR-362 inhibited the proliferation and invasion through binding to the 3'-UTR of SIX1 mRNA in CRC. Additionally, wediscovered that SIX1 was a direct target gene of miR-362 and that the expression of miR-362 had a negative connection with SIX1expression in CRC. SIX1 could reverse partial functions in the proliferation and invasion in CRC cells. Conclusion: miR-362 may be a prognostic marker in CRC and suppress CRC cell proliferation and invasion in part through targetingthe 3'-UTR of SIX1 mRNA. The newly identified miR-362/SIX1 axis provides insight into the progression of CRC.
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