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- 저자 : Chunyu Shi (검색결과 4 건)
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Nan Shi,Hongwei Xu,Kaiyuan Guo,Chunyu Kang,Wei Zhang,Yingying Zhang,Liping Zhang,Jianxin Tan 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.8
Partitioning of microbial transglutaminase (MTG) from Amycolatopsis sp. in the polyethylene glycol (PEG)/salt-based ATPS was investigated for the first time. The key parameters such as the molecular weight of PEG (PEG 600-6000), the type and concentration of phase-forming salt (ammonium sulfate or phosphates), the pH of system (pH 5.0-8.5), and the concentration of neutral salt (0-6% NaCl, w/w) were determined. The partition coefficient of the enzyme was not linear with PEG molecular weight; PEG1000 gave better yield than others. The concentration of PEG1000, ammonium sulfate and NaCl, and the system pH showed effects with different extents on specific activity (SA) and yield of the enzyme. In the ATPS of 26% w/w PEG 1000 and 19% w/w ammonium sulfate in the presence of 5% w/w NaCl and at pH 6.0, MTG was partitioned into the PEG-rich phase with a maximum yield of 86.51% and SA was increased to 0.83. The results of SDS-PAGE showed the MTG produced by the test strain differed from the enzymes reported before. Thus, this study proves that ATPS can be used as a preliminary step for partial purification of MTG from Amycolatopsis sp. fermentation broth.
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A Novel Zero-Voltage-Switching Push-Pull Forward Converter with a Parallel Resonant Network
Chunwei Cai,Chunyu Shi,Yuxing Guo,Zi Yang,Fangang Meng 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.1
A novel zero-voltage-switching (ZVS) push-pull forward converter with a parallel resonant network is presented in this paper. The novel topology can provide a releasing loop for the energy storage in a leakage inductor for the duration of the power switching by the resonant capacitors paralleled with the primary windings of the transformer. Then the transformer leakage inductor is utilized to be resonant with the parallel capacitor, and the ZVS operation is achieved. This converter exhibits many advantages such as lower duty-cycle losses, limited peak voltage across the rectifier diodes and a higher efficiency. Furthermore, the operating principles and key problems of the converter design are analyzed in detail, and the ZVS conditions are derived. A 500W experimental converter prototype has been built to verify the effectiveness of the proposed converter, and its maximum efficiency reaches 94.8%.
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A Novel Zero-Voltage-Switching Push-Pull Forward Converter with a Parallel Resonant Network
Cai, Chunwei,Shi, Chunyu,Guo, Yuxing,Yang, Zi,Meng, Fangang The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.1
A novel zero-voltage-switching (ZVS) push-pull forward converter with a parallel resonant network is presented in this paper. The novel topology can provide a releasing loop for the energy storage in a leakage inductor for the duration of the power switching by the resonant capacitors paralleled with the primary windings of the transformer. Then the transformer leakage inductor is utilized to be resonant with the parallel capacitor, and the ZVS operation is achieved. This converter exhibits many advantages such as lower duty-cycle losses, limited peak voltage across the rectifier diodes and a higher efficiency. Furthermore, the operating principles and key problems of the converter design are analyzed in detail, and the ZVS conditions are derived. A 500W experimental converter prototype has been built to verify the effectiveness of the proposed converter, and its maximum efficiency reaches 94.8%.
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Chanchan Sun,Rui Liu,Tao Wu,Bin Liang,Chunyue Shi,Min Zhang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.5
Effects of superfine grinding on structural and physicochemical properties of whey protein concentrate (WPC) and applications for microparticulated whey protein (MWP) were investigated. WPC and MWP particle sizes significantly (p<0.05) decreased after superfine grinding from 62 and 15 μm to 15 and 9 μm, respectively, compared with controls. A basis for exploration of WPC applications as a fat replacer because of WPC and MWP particle size reductions due to superfine grinding is established. The effect of superfine grinding on molecular configuration, thermal stability, and physicochemical properties of WPC were also investigated. Amounts of free sulfhydryl groups decreased significantly (p<0.05) from 18.16 to 14.49 μmol/L, and thermodynamic properties, including transition temperature, were changed after superfine grinding. The whey protein solubility, protein surface hydrophobicity, oil binding capacity, foaming capacity, and foaming stability of WPC were all improved after superfine grinding.
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