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Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing
Chaoyue Liang,Zhuolin Wu,Xiaohong Gan,Yuanbin Liu,You You,Chenxian Liu,Chengzhi Zhou,Ying Liang,Haiyun Mo,Allen M. Chen,Jiexia Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.1
Purpose: This study aimed to identify potential epidermal growth factor receptor (EGFR) gene mutations in non-small cell lung cancer that went undetected by amplification refractory mutation system-Scorpion real-time PCR (ARMS-PCR). Materials and Methods: A total of 200 specimens were obtained from the First Affiliated Hospital of Guangzhou Medical University from August 2014 to August 2015. In total, 100 ARMS-negative and 100 ARMS-positive specimens were evaluated for EGFR gene mutations by Sanger sequencing. The methodology and sensitivity of each method and the outcomes of EGFR-tyrosine kinase inhibitor (TKI) therapy were analyzed. Results: Among the 100 ARMS-PCR-positive samples, 90 were positive by Sanger sequencing, while 10 cases were considered negative, because the mutation abundance was less than 10%. Among the 100 negative cases, three were positive for a rare EGFR mutation by Sanger sequencing. In the curative effect analysis of EGFR-TKIs, the progression-free survival (PFS) analysis based on ARMS and Sanger sequencing results showed no difference. However, the PFS of patients with a high abundance of EGFR mutationwas 12.4 months [95% confidence interval (CI), 11.6−12.4 months], which was significantly higher than that of patients with a low abundance of mutations detected by Sanger sequencing (95% CI, 10.7−11.3 months) (p<0.001). Conclusion: The ARMS method demonstrated higher sensitivity than Sanger sequencing, but was prone to missing mutations due to primer design. Sanger sequencing was able to detect rare EGFR mutations and deemed applicable for confirming EGFR status. A clinical trial evaluating the efficacy of EGFR-TKIs in patients with rare EGFR mutations is needed.
Liguo Wang,Qingyue Jiang,Chaoyu Zhang,Dongxin Jin,Hui Deng 전력전자학회 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.3
In order to satisfy the urgent requirements for the overheating protection of induction motors, an approach that can be used to identify motor temperature has been proposed based on the rotor slots harmonic (RSH) in this paper. One method to accomplish this is to improve the calculation efficiency of the RSH by predicting the stator winding distribution harmonic order by analyzing the harmonics spectrum. Another approach is to increase the identification accuracy of the RSH by suppressing the influence of voltage flashes or current surges during temperature estimation based on model predictive control (MPC). First, an analytical expression of the stator inductance is extracted from a steady-state positive sequence motor equivalent circuit model developed from the rotor flux field orientation. Then a procedure that applies MPC for reducing the identification error of the rotor temperature caused by voltage sag or swell of the power system is given. Due to this work, the efficiency and accuracy of the RSH have been significantly improved and validated our experiments. This work can serves as a reference for the on-line temperature monitoring and overheating protection of an induction motor.
Wang, Liguo,Jiang, Qingyue,Zhang, Chaoyu,Jin, Dongxin,Deng, Hui The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.3
In order to satisfy the urgent requirements for the overheating protection of induction motors, an approach that can be used to identify motor temperature has been proposed based on the rotor slots harmonic (RSH) in this paper. One method to accomplish this is to improve the calculation efficiency of the RSH by predicting the stator winding distribution harmonic order by analyzing the harmonics spectrum. Another approach is to increase the identification accuracy of the RSH by suppressing the influence of voltage flashes or current surges during temperature estimation based on model predictive control (MPC). First, an analytical expression of the stator inductance is extracted from a steady-state positive sequence motor equivalent circuit model developed from the rotor flux field orientation. Then a procedure that applies MPC for reducing the identification error of the rotor temperature caused by voltage sag or swell of the power system is given. Due to this work, the efficiency and accuracy of the RSH have been significantly improved and validated our experiments. This work can serves as a reference for the on-line temperature monitoring and overheating protection of an induction motor.
Chao Yu,Jian Lu,Qi Zhang,Hougang Fan,Minjia Meng,Shi Zhou,Yinhua Jiang,Yongsheng Yan,Yilin Wu,Chunxiang Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.2
High purity emodin is in great demand with the development of medical treatment. Molecularly imprinted membranes (MIMs) have gained wide attention for selective separation of emodin due to its preferable selectivity. In this work, we describe a simple two-step method for developing emodin-imprinted TiO2@CA (ETMIMs) and emodin-imprinted SiO2@CA (ESMIMs) based on organic–inorganic nanoparticle (SiO2/TiO2) modified cellulose acetate membranes at room temperature. SiO2/TiO2 is used to improve the structural stability and roughness of membranes, and dopamine is used as the functional monomer and crosslinker. Importantly, the as-prepared membranes not only exhibited enhanced rebinding capacity (ETMIMs = 30.73 mg g -1 and ESMIMs = 46.04 mg g -1) but also possessed superior rebinding selectivity (2.76 and 2.99 for physcion and 2.42 and 3.30 for aloe emodin onto ETMIMs and ESMIMs) as well as permselectivity (7.59 and 6.69 for physcion and 5.94 and 5.78 for aloe emodin onto ETMIMs and ESMIMs). The regeneration ability of ETMIMs and ESMIMs still maintained 90.4% and 89.2% of the original rebinding capacity after 10 cycling steps, respectively. The ETMIMs and ESMIMs obtained in this work show potential applications for selective separation and purification of emodin from analogs.