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Yu, Changsong,Jia, Gang,Jiang, Yi,Deng, Qiuhong,Chen, Zhengli,Xu, Zhiwen,Chen, Xiaolin,Wang, Kangning Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.5
The glucagon-like peptide 2 (GLP-2) that is expressed in intestine epithelial cells of mammals, is important for intestinal barrier function and regulation of tight junction (TJ) proteins. However, there is little known about the intracellular mechanisms of GLP-2 in the regulation of TJ proteins in piglets' intestinal epithelial cells. The purpose of this study is to test the hypothesis that GLP-2 regulates the expressions of TJ proteins in the mitogen-activated protein kinase (MAPK) signaling pathway in piglets' intestinal epithelial cells. The jejunal tissues were cultured in a Dulbecco's modified Eagle's medium/high glucose medium containing supplemental 0 to 100 nmol/L GLP-2. At 72 h after the treatment with the appropriate concentrations of GLP-2, the mRNA and protein expressions of zonula occludens-1 (ZO-1), occludin and claudin-1 were increased (p<0.05). U0126, an MAPK kinase inhibitor, prevented the mRNA and protein expressions of ZO-1, occludin, claudin-1 increase induced by GLP-2 (p<0.05). In conclusion, these results indicated that GLP-2 could improve the expression of TJ proteins in weaned pigs' jejunal epithelium, and the underlying mechanism may due to the MAPK signaling pathway.
Yu, Changsong,Jia, Gang,Deng, Qiuhong,Zhao, Hua,Chen, Xiaoling,Liu, Guangmang,Wang, Kangning Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5
Glucagon-like peptide-2 (GLP-2) is important for intestinal barrier function and regulation of tight junction (TJ) proteins, but the intracellular mechanisms of action remain undefined. The purpose of this research was to determine the protective effect of GLP-2 mediated TJ and transepithelial electrical resistance (TER) in lipopolysaccharide (LPS) stressed IPEC-J2 cells and to test the hypothesis that GLP-2 regulate TJ and TER through the phosphatidylinositol 3-kinase (PI3K)-protein kinase B (Akt)-mammalian target of rapamycin (mTOR) signaling pathway in IPEC-J2 cells. Wortmannin and LY294002 are specific inhibitors of PI3K. The results showed that $100{\mu}g/mL$ LPS stress decreased TER and TJ proteins occludin, claudin-1 and zonula occludens protein 1 (ZO-1) mRNA, proteins expressions (p<0.01) respectively. GLP-2 (100 nmol/L) promote TER and TJ proteins occludin, claudin-1, and zo-1 mRNA, proteins expressions in LPS stressed and normal IPEC-J2 cells (p<0.01) respectively. In normal cells, both wortmannin and LY294002, PI3K inhibitors, prevented the mRNA and protein expressions of Akt and mTOR increase induced by GLP-2 (p<0.01) following with the significant decreasing of occludin, claudin-1, ZO-1 mRNA and proteins expressions and TER (p<0.01). In conclusion, these results indicated that GLP-2 can promote TJ's expression and TER in LPS stressed and normal IPEC-J2 cells and GLP-2 could regulate TJ and TER through the PI3K/Akt/mTOR pathway.
Guozhen Hu,Changsong Chen,Duan Shanxu 전력전자학회 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.2
The use of LCL filters in pulse width modulation voltage source converters is a standard solution for providing proper attenuation of high-order grid-current harmonics. However, these filters can cause the undesired effect of resonance. This paper proposes an active damping strategy with harmonics compensation. It can alleviate the harmonics around the resonance frequency caused by the LCL filters. The proposed strategy is attractive since it is simple, does not depend on grid parameters and does not increase the number of sensors. Simulation and experimental results verify the effectiveness of the proposed active damping strategy.
Hu, Guozhen,Chen, Changsong,Shanxu, Duan The Korean Institute of Power Electronics 2013 JOURNAL OF POWER ELECTRONICS Vol.13 No.2
The use of LCL filters in pulse width modulation voltage source converters is a standard solution for providing proper attenuation of high-order grid-current harmonics. However, these filters can cause the undesired effect of resonance. This paper proposes an active damping strategy with harmonics compensation. It can alleviate the harmonics around the resonance frequency caused by the LCL filters. The proposed strategy is attractive since it is simple, does not depend on grid parameters and does not increase the number of sensors. Simulation and experimental results verify the effectiveness of the proposed active damping strategy.