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        Prokaryotic expression and protein function of Brassica napus PGIP2 and its genetic transformation

        Haiyan HuangFu,Chunyun Guan,Furong Jin,Changfa Yin 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.2

        Sclerotinia rot is a fungal disease caused bySclerotinia sclerotiorum (Lib.) de Bary, which has severelyreduced rapeseed production worldwide. Polygalacturonase-inhibiting proteins (PIGPs) inhibit theactivity of polygalacturonases, which are secreted duringfungal infection in plants. This study investigated thefunction of the polygalacturonase-inhibitor gene 2 (PGIP2)in sclerotinia rot resistance. The PGIP2 was successfullyexpressed in a prokaryotic system, and recombinant PGIP2protein, purified after enterokinase treatment to remove tagpeptide, inhibited S. sclerotiorum PG activity in vitro. PGIP2 was overexpressed in the susceptible Brassica napuscultivar 98c40 via Agrobacterium-mediated transformation. After inoculation with S. sclerotiorum mycelia, thetransgenic rapeseed demonstrated greatly reduced leafdamage, as compared with their non-transgenic plants. Therefore, the PGIP2 encodes a functional protein andwould be a candidate gene for enhancing Sclerotinia rotresistance.

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