http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Prokaryotic expression and protein function of Brassica napus PGIP2 and its genetic transformation
Haiyan HuangFu,Chunyun Guan,Furong Jin,Changfa Yin 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.2
Sclerotinia rot is a fungal disease caused bySclerotinia sclerotiorum (Lib.) de Bary, which has severelyreduced rapeseed production worldwide. Polygalacturonase-inhibiting proteins (PIGPs) inhibit theactivity of polygalacturonases, which are secreted duringfungal infection in plants. This study investigated thefunction of the polygalacturonase-inhibitor gene 2 (PGIP2)in sclerotinia rot resistance. The PGIP2 was successfullyexpressed in a prokaryotic system, and recombinant PGIP2protein, purified after enterokinase treatment to remove tagpeptide, inhibited S. sclerotiorum PG activity in vitro. PGIP2 was overexpressed in the susceptible Brassica napuscultivar 98c40 via Agrobacterium-mediated transformation. After inoculation with S. sclerotiorum mycelia, thetransgenic rapeseed demonstrated greatly reduced leafdamage, as compared with their non-transgenic plants. Therefore, the PGIP2 encodes a functional protein andwould be a candidate gene for enhancing Sclerotinia rotresistance.