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        Biocompatibility and bioactive potential of the NeoMTA Plus endodontic bioceramic-based sealer

        Hoshino Roberto Alameda,Delfino Mateus Machado,da Silva Guilherme Ferreira,Guerreiro-Tanomaru Juliane Maria,Tanomaru-Filho Mário,Sasso-Cerri Estela,Cerri Paulo Sérgio 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.1

        Objectives: This study evaluated the biocompatibility and bioactive potential of NeoMTA Plus mixed as a root canal sealer in comparison with MTA Fillapex. Materials and Methods: Polyethylene tubes filled with NeoMTA Plus (n = 20), MTA Fillapex (n = 20), or nothing (control group, CG; n = 20) were inserted into the connective tissue in the dorsal subcutaneous layer of rats. After 7, 15, 30 and 60 days, the specimens were processed for paraffin embedding. The capsule thickness, collagen content, and number of inflammatory cells (ICs) and interleukin-6 (IL-6) immunolabeled cells were measured. von Kossa-positive structures were evaluated and unstained sections were analyzed under polarized light. Two-way analysis of variance was performed, followed by the post hoc Tukey test (p ≤ 0.05). Results: At 7 days, the capsules around NeoMTA Plus and MTA Fillapex had more ICs and IL-6-immunostained cells than the CG. However, at 60 days, there was no significant difference in the IC number between NeoMTA Plus and the CG (p = 0.1137) or the MTA Fillapex group (p = 0.4062), although a greater number of IL-6-immunostained cells was observed in the MTA Fillapex group (p = 0.0353). From 7 to 60 days, the capsule thickness of the NeoMTA Plus and MTA Fillapex specimens significantly decreased, concomitantly with an increase in the collagen content. The capsules around root canal sealers showed positivity to the von Kossa stain and birefringent structures. Conclusions: The NeoMTA Plus root canal sealer is biocompatible and exhibits bioactive potential.

      • SCISCIESCOPUS

        Effects of resynchronization programs on pregnancy per artificial insemination, progesterone, and pregnancy-associated glycoproteins in plasma of lactating dairy cows

        Thompson, I.M.,Cerri, R.L.A.,Kim, I.H.,Green, J.A.,Santos, J.E.P.,Thatcher, W.W. American Dairy Science Association 2010 Journal of dairy science Vol.93 No.9

        Objectives were to develop a timed artificial insemination (TAI) resynchronization program to improve pregnancy per AI and to evaluate responses of circulating progesterone and pregnancy-associated glycoproteins in lactating cows. Cows (n=1,578) were presynchronized with 2 injections of PGF<SUB>2α</SUB>, given 14 d apart starting on d 45+/-3 postpartum, followed by Ovsynch [2 injections of GnRH 7 d before and 56h after injection of PGF<SUB>2α</SUB>, TAI 16h after second injection (d 0)]. The Resynch-treated cows received an intravaginal progesterone insert from d 18 to 25, GnRH on d 25, and pregnancy diagnosis on d 32, and nonpregnant cows received PGF<SUB>2α.</SUB>, GnRH 56h later, and TAI 16h later (d 35). The control cows were diagnosed for pregnancy on d 32 and nonpregnant cows received GnRH, PGF<SUB>2α</SUB> 39 d after TAI, GnRH 56h later, and TAI 16h later (d 42). Pregnancy was reconfirmed on d 60 after AI. Ovarian structures were examined in a subset of cows at the time of GnRH and PGF<SUB>2α</SUB> injections. Blood samples for analyses of progesterone and pregnancy-associated glycoproteins were collected every 2 d from d 18 to 30 in 100 cows, and collection continued weekly to d 60 for pregnant cows (n=43). Preenrollment pregnancies per AI on d 32 did not differ for cows subsequently treated as Resynch (45.8%, n=814) and control (45.9%, n=764), and pregnancy losses on d 60 were 6.7 and 4.0%, respectively. Resynchronized service pregnancy per AI (36%, n=441; 39.5%, n=412) and pregnancy losses (6.3 and 6.7%) did not differ for Resynch and control treatments, respectively. Days open for pregnant cows after 2 TAI were less for the Resynch treatment than for the control treatment (96.2+/-0.82 vs. 99.5+/-0.83 d). Cows in the Resynch treatment had more large follicles at the time of GnRH. The number of corpora lutea did not differ between treatments at the time of PGF<SUB>2α</SUB>. Plasma progesterone for pregnant cows was greater for Resynch cows than for control cows (18-60 d; 6.6 vs. 5.3ng/mL), and plasma concentrations of progesterone on d 18 were greater for pregnant cows than for nonpregnant cows (5.3 vs. 4.3ng/mL). Plasma pregnancy-associated glycoproteins during pregnancy were lower for cows in the Resynch treatment compared with control cows on d 39 (2.8 vs. 4.1ng/mL) and 46 (1.3 vs. 3.0ng/mL). Cows pregnant on d 32 that lost pregnancy by d 60 (n=7) had lower plasma concentrations of pregnancy-associated glycoproteins on d 30 than cows that maintained pregnancy (n=36; 2.9 vs. 5.0ng/mL). Pregnancy-associated glycoproteins on d 30 (>0.33ng/mL) were predictive of a positive d 32 pregnancy diagnosis (sensitivity=100%; specificity=90.6%). In conclusion, Resynch and control protocols had comparable pregnancy per AI for first and second TAI services, but pregnancy occurred 3.2 d earlier in the Resynch group because inseminations in the Resynch treatment began 7 d before those in the control treatment. Administration of an intravaginal progesterone insert, or GnRH, or both increased progesterone during pregnancy. Dynamics of pregnancy-associated glycoproteins were indicative of pregnancy status and pregnancy loss.

      • 자동 주차를 위한 차량 조향 및 속도제어 알고리즘 개발

        윤지환(Jihwan Yoon),이경수(Kyongsu Yi),Pietro Cerri,Albetro Broggi 한국자동차공학회 2012 한국자동차공학회 학술대회 및 전시회 Vol.2012 No.11

        본 논문에서는 자동 주차를 위한 차량 제어 알고리즘을 다루고 있다. Global Positioning System(GPS) 정보로부터 차량의 위치와 heading angle, 주차 구역의 위치와 각도를 알 수 있다. 이를 기반으로 후진 시작 지점인 Middle Point를 결정하고, 현재 차량의 위치에서부터 Middle Point까지의 경로와 Middle Point에서부터 Goal Point까지의 경로를 현재 차량의 위치와 heading angle, 목적 지점의 위치와 각도를 구속조건으로 하여 3차 곡선으로 생성한다. 경로를 따르기 위한 조향각은 임계값을 넘지 않아야 한다. GPS 정보를 이용하여 주차 구역 근처의 지점으로 이동한다. 그리고 차량 후미에 Camera를 장착하여 후진 상황에서 주차구역을 인지하고 정확한 Goal Point와 Middle Point를 결정한다. 기어 변속을 위한 장치로서 Automatic Transmission Shifting Control (ATSC)을 사용하였다. 다양한 지점에서 주차가 가능한 경로를 생성하기 위해 시뮬레이션을 통해 검증하였고, 시험 차량을 통해 실제 차량에서의 안정적인 주차가 가능함을 검증하였다.

      • Environment-Detection-and-Mapping Algorithm for Autonomous Driving in Rural or Off-Road Environment

        Jaewoong Choi,Junyoung Lee,Dongwook Kim,Soprani, G.,Cerri, P.,Broggi, A.,Kyongsu Yi IEEE 2012 IEEE transactions on intelligent transportation sy Vol.13 No.2

        <P>This paper presents an environment-detection-and-mapping algorithm for autonomous driving that is provided in real time and for both rural and off-road environments. Environment-detection-and-mapping algorithms have been designed to consist of two parts: (1) lane, pedestrian-crossing, and speed-bump detection algorithms using cameras and (2) obstacle detection algorithm using LIDARs. The lane detection algorithm returns lane positions using one camera and the vision module “VisLab Embedded Lane Detector (VELD),” and the pedestrian-crossing and speed-bump detection algorithms return the position of pedestrian crossings and speed bumps. The obstacle detection algorithm organizes data from LIDARs and generates a local obstacle position map. The designed algorithms have been implemented on a passenger car using six LIDARs, three cameras, and real-time devices, including personal computers (PCs). Vehicle tests have been conducted, and test results have shown that the vehicle can reach the desired goal with the proposed algorithm.</P>

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