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Naik, Shalin H,Sathe, Priyanka,Park, Hae-Young,Metcalf, Donald,Proietto, Anna I,Dakic, Aleksander,Carotta, Sebastian,O'Keeffe, Meredith,Bahlo, Melanie,Papenfuss, Anthony,Kwak, Jong-Young,Wu, Li,Shortm NATURE AMERICA INC 2007 NATURE IMMUNOLOGY Vol.8 No.11
The development of functionally specialized subtypes of dendritic cells (DCs) can be modeled through the culture of bone marrow with the ligand for the cytokine receptor Flt3. Such cultures produce DCs resembling spleen plasmacytoid DCs (pDCs), CD8<SUP>+</SUP> conventional DCs (cDCs) and CD8<SUP>−</SUP> cDCs. Here we isolated two sequential DC-committed precursor cells from such cultures: dividing 'pro-DCs', which gave rise to transitional 'pre-DCs' en route to differentiating into the three distinct DC subtypes (pDCs, CD8<SUP>+</SUP> cDCs and CD8<SUP>−</SUP> cDCs). We also isolated an in vivo equivalent of the DC-committed pro-DC precursor cell, which also gave rise to the three DC subtypes. Clonal analysis of the progeny of individual pro-DC precursors demonstrated that some pro-DC precursors gave rise to all three DC subtypes, some produced cDCs but not pDCs, and some were fully committed to a single DC subtype. Thus, commitment to particular DC subtypes begins mainly at this pro-DC stage.
SCON—a Short Conditional intrON for conditional knockout with one-step zygote injection
Wu Szu-Hsien Sam,Lee Heetak,Szép-Bakonyi Réka,Colozza Gabriele,Boese Ayse,Gert Krista R.,Hallay Natalia,Lee Ji-Hyun,Kim Jihoon,Zhu Yi,Linssen Margot M.,Pilat-Carotta Sandra,Hohenstein Peter,Theussl Ha 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection. In this study, a total of 13 SCON mouse lines were successfully generated by 2 different laboratories. SCON has conditional intronic functions in various vertebrate species, and its target insertion is as simple as CRISPR/Cas9-mediated gene tagging.