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Isolation and Characterization of Antifungal Peptides Produced by Bacillus amyloliquefaciens LBM5006
Lisianne Brittes Benitez,Renata Voltolini Velho,Marcia Pagno Lisboa,Luis Fernando da Costa Medina,Adriano Brandelli 한국미생물학회 2010 The journal of microbiology Vol.48 No.6
Bacillus amyloliquefaciens LBM 5006 produces antagonistic activity against pathogenic bacteria and phytopathogenic fungi, including Aspergillus spp., Fusarium spp., and Bipolaris sorokiniana. PCR analysis revealed the presence of ituD, but not sfp genes, coding for iturin and surfactin, respectively. The antimicrobial substance produced by this strain was isolated by ammonium sulfate precipitation, gel filtration chromatography and 1-butanol extraction. The ultraviolet spectrum was typical of a polypeptide and the infrared spectrum indicates the presence of peptide bonds and acyl group(s). The antimicrobial substance was resistant to proteolytic enzymes and heat treatment, and was reactive with ninhydrin. Mass spectroscopy analysis indicated that B. amyloliquefaciens LBM 5006 produces two antimicrobial peptides,with main peaks at m/z 1,058 Da and 1,464 Da, corresponding to iturin-like and fengycin-like peptides,respectively. B. amyloliquefaciens LBM 5006 showed significant activity against phytopatogenic fungi, showing potential for use as a biocontrol agent or production of antifungal preparations.
Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
Alfredo García,Remigio Martínez,José Manuel Benitez-Medina,David Risco,Waldo Luis García,Joaquín Rey,Juan Manuel Alonso,Javier Hermoso de Mendoza 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.4
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa,Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis.
Investigation of radioactivity-induced backgrounds in EXO-200
Albert, J. B.,Auty, D. J.,Barbeau, P. S.,Beck, D.,Belov, V.,Benitez-Medina, C.,Breidenbach, M.,Brunner, T.,Burenkov, A.,Cao, G. F.,Chambers, C.,Cleveland, B.,Coon, M.,Craycraft, A.,Daniels, T.,Danilov American Physical Society 2015 PHYSICAL REVIEW C - Vol.92 No.1