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        Expression of an Arabidopsis sodium/proton antiporter gene (AtNHX1) in peanut to improve salt tolerance

        Manoj Banjara,Longfu Zhu,Guoxin Shen,Paxton Payton,Hong Zhang 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.1

        Salinity is a major environmental stress that affects agricultural productivity worldwide. One approach to improving salt tolerance in crops is through high expression of the Arabidopsis gene AtNHX1, which encodes a vacuolar sodium/proton antiporter that sequesters excess sodium ion into the large intracellular vacuole. Sequestering cytosolic sodium into the vacuoles of plant cells leads to a low level of sodium in cytosol, which minimizes the sodium toxicity and injury to important enzymes in cytosol. In the meantime, the accumulation of sodium in vacuoles restores the correct osmolarity to the intracellular milieu, which favors water uptake by plant root cells and improves water retention in tissues under soils that are high in salt. To improve the yield and quality of peanut under high salt conditions, AtNHX1 was introduced into peanut plants through Agrobacterium-mediated transformation. The AtNHX1-expressing peanut plants displayed increased tolerance of salt at levels up to 150 mM NaCl. When compared to wild-type plants, AtNHX1-expressing peanut plants suffered less damage, produced more biomass, contained more chlorophyll, and maintained higher photosynthetic rates under salt conditions. These data indicate that AtNHX1 can be used to enhance salt tolerance in peanut.

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        Damage progression study in fibre reinforced concrete using acoustic emission technique

        Nawal Kishor Banjara,Saptarshi Sasmal,V. Srinivas 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.23 No.2

        The main objective of this study is to evaluate the true fracture energy and monitor the damage progression in steel fibre reinforced concrete (SFRC) specimens using acoustic emission (AE) features. Four point bending test is carried out using pre-notched plain and fibre reinforced (0.5% and 1% volume fraction) - concrete under monotonic loading. AE sensors are affixed at different locations of the specimens and AE parameters such as rise time, AE energy, hits, counts, amplitude and duration etc. are obtained. Using the captured and processed AE event data, fracture process zone is identified and the true fracture energy is evaluated. The AE data is also employed for tracing the damage progression in plain and fibre reinforced concrete, using both parametric- and signal- based techniques. Hilbert - Huang transform (HHT) is used in signal based processing for evaluating instantaneous frequency of the acoustic events. It is found that the appropriately processed and carefully analyzed acoustic data is capable of providing vital information on progression of damage on different types of concrete.

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        Effective CFRP retrofit strategy for flexural deficient RC beams

        Nawal Kishor Banjara,K. Ramanjaneyulu 국제구조공학회 2019 Structural Engineering and Mechanics, An Int'l Jou Vol.69 No.2

        Structural deterioration arises due to aging, environmental effects, deficiencies during design and construction phase, and overloading. Experimental and numerical investigations are carried out in this study to evaluate the performance of control and flexural deficient reinforced concrete (RC) beams under monotonic loading. Three levels of flexural deficiency are considered in this study. After confirming load carrying capacities of control and flexural deficient beams, the flexural deficient RC beams are strengthened with carbon fibre reinforced polymer (CFRP) fabric. CFRP strengthened RC beams are tested under monotonic loading and compared with the performance of control specimen. Further, non-linear finite element analyses are also carried out to evaluate the flexural performance of control, deficient and CFRP strengthened flexural deficient RC beams. There is good correlation between results of experimental and numerical investigations. Numerical approach presented in this study can be adopted for assessing the adequacy of CFRP retrofit measure.

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        Pretreatment of agricultural biomass for anaerobic digestion: Current state and challenges

        Paudel, Shukra Raj,Banjara, Sushant Prasad,Choi, Oh Kyung,Park, Ki Young,Kim, Young Mo,Lee, Jae Woo Elsevier Applied Science 2017 Bioresource technology Vol.245 No.1

        <P><B>Abstract</B></P> <P>The anaerobic digestion (AD) of agricultural biomass is an attractive second generation biofuel with potential environmental and economic benefits. Most agricultural biomass contains lignocellulose which requires pretreatment prior to AD. For optimization, the pretreatment methods need to be specific to the characteristics of the biomass feedstock. In this review, cereal residue, fruit and vegetable wastes, grasses and animal manure were selected as the agricultural biomass candidates, and the fundamentals and current state of various pretreatment methods used for AD of these feedstocks were investigated. Several nonconventional methods (electrical, ionic liquid-based chemicals, ruminant biological pretreatment) offer potential as targeted pretreatments of lignocellulosic biomass, but each comes with its own challenges. Pursuing an energy-intensive route, a combined bioethanol-biogas production could be a promising a second biofuel refinery option, further emphasizing the importance of pretreatment when lignocellulosic feedstock is used.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pretreatment is guided for anaerobic digestion of agricultural biomass. </LI> <LI> Characteristics of feedstock must be considered for optimizing pretreatment. </LI> <LI> Alkali and steam explosion leads to an enhanced anaerobic digestion performance. </LI> <LI> PEF, ionic liquids and rumen system can be future challenges for pretreatment. </LI> <LI> Pretreatment is significant in a combined biorefinery with agricultural residue. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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