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- 저자 : B.P. Singh (검색결과 107 건)
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Jeon, S.Y.,Singh, B.,Im, H.N.,Seong, K.P.,Song, S.J. Elsevier Sequoia 2014 Journal of alloys and compounds Vol.589 No.-
<P>In this work, mass and charge transport properties of acceptor doped lanthanum nickelate, La2Ni0.95Al0.05O4.025+delta d (LNAO), were analyzed. The thermal expansion, electrical conductivity and thermoelectric power of LNAO were measured as a function of temperature in 25-1000 degrees C range and oxygen partial pressure ( pO(2)) in -14 <= log (pO(2)/atm)6 <= -1 range. The average thermal expansion coefficient was 13.77 x 10 (6) K (1). The electrical conductivity was analyzed in relation to the thermoelectric power to elucidate the positive deviation of the activity coefficient of hole on the basis of the delocalized electron model. The thermoelectric power measurement shows a p-type to n- type transition. The chemical diffusion coefficient (D-chem) and surface exchange coefficient (ksurf.) were calculated by 4-probe DC conductivity measurement and ksurf. was slightly higher than ((D) over tilde Dchem). The best- estimated hole-mobility values showed very weak temperature dependence. The results were compared with the literature results on La2NiO4+ d. (C) 2013 Elsevier B.V. All rights reserved.</P>
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Singh, Rani,Jain, Preeti,Pandey, N.K.,Saxena, V.K.,Saxena, M.,Singh, K.B.,Ahmed, K.A.,Singh, R.P. Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.7
In the present study, the impact of Salmonella Typhimurium on cell-mediated immunity (CMI) was investigated in 5 week-old immuno divergent broiler lines selected for the high and low response to phytohemagglutinin-P. The immune response was assessed in peripheral-blood mononuclear cells (PBMCs) induced with Salmonella Typhimurium at different time intervals (0 h, 0.5 h, 2 h, 4 h, 6 h, 12 h and 24 h). The differential mRNA expression patterns of IFN-${\gamma}$, IL-2 and iNOS were evaluated by quantitative real time PCR. In-vitro production of nitric oxide (NO) was also estimated in the culture supernatant and correlated with iNOS mRNA expression. Present study showed higher production of NO in the high cell-mediated line (HCMI) as compared to the low cell-mediated line (LCMI) upon stimulation with Salmonella Typhimurium. Correspondingly, higher mRNA expression of iNOS and IFN-${\gamma}$ were observed in high response birds (HCMI); but IL-2 was down regulated in this line compared to the low response birds (LCMI). Significantly (p<0.05) higher expression of iNOS, IFN-${\gamma}$ and higher production of NO in high line indicated that the selection for PHA-P response might be employed for increasing the immune competence against Salmonella Typhimurium in chicken flocks.
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Singh, G.P.,Zeya, S.B.,Srivastava, A.K.,Ojha, N.G.,Suryanarayana, N. Korean Society of Sericultural Science 2008 International Journal of Industrial Entomology Vol.17 No.1
Pathogenic infection in tasar silkworm, Antheraea mylitta Drury is common and there is a breed specific response regarding tolerance. Hence, the tolerance level of three eco-races of tasar silkworm viz. Daba, Sarihan and Raily to Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) infection was tested. The survival of Daba, Sarihan and Raily eco-races was significantly different (p<0.05) when challenged with the same concentration ($1{\times}10^5$ polyhedra/ml) of AmCPV. Daba eco-race was more tolerant to the AmCPV infection having higher survival (65.7%) and $LC_{50}$ values (1000893.17 polyhedra/ml) of AmCPV followed by Sarihan eco-race (50.7% survival and $LC_{50}$ value of AmCPV 187203.6168 polyhedra/ml) and Raily eco-race (24.3% survival and $LC_{50}$ value of AmCPV 5176.37 polyhedra/ml). The tolerance level of Daba and Sarihan eco-races against AmCPV was more than two times than Raily eco-race.
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Serum and CSF Mineral Profile of Himalayan Yak (Bas grunniens) in their Natural Habitat
Singh, S.P.,Kumar, N.,Sharma, K.B.,Kumar, R. Asian Australasian Association of Animal Productio 1999 Animal Bioscience Vol.12 No.2
Mineral profile of serum and cerebrospinal fluid (CSF) of 15 Himalayan Yak (adult female, n=8; adult male n=4 and young male,n=3) was studied in their natural habitat at an altitude of 3300 meters above mean sea level at Sangla in north western Himalayas. The macro and micro minerals estimated in serum and CSF were; Sodium, Potassium, Calcium and Magnesium and Zinc, Copper and Iron respectively. The values recorded among different Yak groups did not significantly differ from each other except serum iron and haemoglobin which were significantly higher (p<0.05 ) in young male Yaks compared to the adult male and female Yaks. An observation of great significance was considerably higher potassium and lower sodium level in Yaks compared to other bovine species of plains. The serum potassium values in some adult female Yaks were recorded as high as 10.4 mEq/l and the values varied between 6.6 to 9.8 mEq/l in young male Yaks. The serum Sodium values and Na:K ratios in Yak serum ranged between 117.5 to 122.6 mEq/l and 13.7 to 15.3 respectively. The possible relationship of high serum Potassium value with hypoxic conditions and hostile mountain environment has been discussed.
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Pradeep Singh,Vikas Shrivastava,I. B. Singh,D. P. Mondal 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6
Three group of porous Ti4Al4Co samples made of (i) unmilled powder and vacuum sintered (S1), (ii) milled powder andvacuum sintered (S2), and (iii) milled powder sintered in open atmosphere (S3) were prepared. Phase change, crystallite sizeand lattice strain variations due to powder milling and sintering were examined by SEM and XRD. To evaluate mechanicalproperty, alloy samples were subjected to the uniaxial compression test. For corrosion analysis, Tafel plot was plotted usingelectrochemical corrosion system in simulated body fluid (SBF) as electrolytic medium. From the obtained results, it wasfound that significant microstructural transformation takes place due to milling and change in sintering atmosphere. SampleS1possessed 139 MPa compressive strength, which was about 48% and 76% higher than samples S2and S3respectively. Corrosion current density for S3was found as 5.5 ± 0.3 μA/cm2 which is 7 and 12 times lower than S2and S1samples.
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( G. P. Singh ),( S. B. Zeya ),( A. K. Srivastava ),( N. G. Ojha ),( N. Suryanarayana ) 한국잠사학회 2008 International Journal of Industrial Entomology Vol.17 No.1
Pathogenic infection in tasar silkworm, Antheraea mylitta Drury is common and there is a breed specific response regarding tolerance. Hence, the tolerance level of three eco-races of tasar silkworm viz. Daba, Sarihan and Raily to Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) infection was tested. The survival of Daba, Sarihan and Raily eco-races was significantly different (p<0.05) when challenged with the same concentration (1 x 10(5) polyhedra/ml) of AmCPV. Daba eco-race was more tolerant to the AmCPV infection having higher survival (65.7%) and LC50 values (1000893.17 polyhedra/ml) of AmCPV followed by Sarihan eco-race (50.7% survival and LC50 value of AmCPV 187203.6168 polyhedra/ml) and Raily ecorace (24.3% survival and LC50 value of AmCPV 5176.37 polyhedra/ml). The tolerance level of Daba and Sarihan eco-races against AmCPV was more than two times than Raily eco-race.
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Malik, Y.P.S.,Chakravarti, S.,Sharma, K.,Vaid, N.,Rajak, K.K.,Balamurugan, V.,Biswas, S.K.,Mondal, B.,Kataria, R.S.,Singh, R.K. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.7
Toll-like receptors (TLRs) play an important role in the recognition of invading pathogens and the modulation of innate immune responses in mammals. The TLR4 and TLR7 are well known to recognize the bacterial lipopolysaccharide (LPS) and single stranded (ssRNA) ligands, respectively and play important role in host defense against Gram-negative bacteria and ssRNA viruses. In the present study, coding exon fragments of these two TLRs were identified, cloned, sequenced and analyzed in terms of insertion-deletion polymorphism, within bovine TLRs 4 and 7, thereby facilitating future TLR signaling and association studies relevant to bovine innate immunity. Comparative sequence analysis of TLR 4 exons revealed that this gene is more variable, particularly the coding frame (E3P1), while other parts showed percent identity of 95.7% to 100% at nucleotide and amino acid level, respectivley with other Bos indicus and Bos taurus breeds from different parts of the world. In comparison to TLR4, sequence analysis of TLR7 showed more conservation among different B. indicus and B. taurus breeds, except single point mutation at 324 nucleotide position (AAA to AAM) altering a single amino acid at 108 position (K to X). Percent identity of TLR7 sequences (all 3 exons) was between 99.2% to 100% at nucleotide and amino acid level, when compared with available sequence database of B. indicus and B. taurus. Simple Modular Architecture Research Tool (SMART) analysis showed variations in the exon fragments located in the Leucine Rich Repeat (LRR) region, which is responsible for binding with the microbial associated molecular patterns and further, downstream signaling to initiate anti-microbial response. Considering importance of TLR polymorphism in terms of innate immunity, further research is warranted.
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Singh, P.,Lee, H.C.,Silva, M.F.,Chin, K.B.,Kang, I. Elsevier 2017 FOOD CONTROL Vol.77 No.-
<P>The purpose of this research was to evaluate the effects of trisodium phosphate dip (TSP), hot water dip (HWD) and their combination dip with/without brushing on broiler breast skin for bacterial reduction and structural changes. Eviscerated broiler carcasses were obtained from a local slaughter plant and immediately subjected to one of six treatments: 1) two tap water dips at 25 degrees C (TWD/TWD), 2) TWD/ TWD with brushing (TWD/TWD/B), 3) TWD and TSP at 8%/25 degrees C (TWD/TSP), 4) TWD and HWD at 71 degrees C (TWD/HWD), 5) TSP and HWD (TSP/HWD), and 6) TSP/HWD with brushing (TSPIHWD/B). Each dip was conducted for 45 s with or without brushing at 5 s on/off during the second dip. Compared to the control (TWD/TWD), TSP/HWD significantly reduced mesophilic aerobic bacteria (MAB), Escherichia coli (E. coli), and total coliforms by 1.1, 0.9 and 1.0 log CFU/g, respectively, and Salmonella prevalence by 53.3% (P < 0.05), whereas TWD/TSP and TWD/HWD showed intermediate reductions (P < 0.05). Upon brushing, TSP/HWD/B reduced populations of MAB, E. coli, and total coliforms, and the prevalence of Salmonella more effectively than control of brushing (TWID/TWD/B) (P < 0.05). When two sampling methods were compared, the method of stomaching released fewer MAB and total coliforms (named loosely-associated cells) than the grinding of the stomached skin (named tightly-associated cells). Compared to controls (TWD/TWD and TWD/TWD/B), both TSP/HWD and TSP/HWD/B generally resulted in darker, less reddish, and more yellowish breast skin. Scanning electron microscope and histological images indicated that both TSP/HWD and TSP/HWD/B had deeper skin penetration than controls or TWD/HWD and TWD/TSP. Overall, TSP/HWD/B showed the most effectiveness in broiler carcass decontamination. (C) 2017 Published by Elsevier Ltd.</P>
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Mishra, R K,Upadhyay, S B,Kushwaha, Ajay,Kim, Tae-Hyung,Murali, G,Verma, Ranjana,Srivastava, Manish,Singh, Jay,Sahay, P P,Lee, Seung Hee RSC Pub 2015 Nanoscale Vol.7 No.28
<P>We report the H2 and LPG gas sensing behavior of RGO/SnO2 QDs synthesized by a surfactant assisted hydrothermal method. The RGO/SnO2 QD based sensor shows a high response of 89.3% to H2 and 92.4% to LPG for 500 ppm test gas concentration at operating temperatures of 200 C and 250 C, respectively. Further, the RGO/SnO2 QD based sensor shows good selectivity for H2 and LPG in the presence of other interfering gases such as ammonia, chloroform, toluene, benzene, acetone, n-butylacetate, acetic acid and formic acid. We observed that the gas response to H2 is 29.8 times higher than that to acetic acid whereas the gas response to LPG is 17.8 times higher than that to formic acid. Long-term analyses have also been performed to demonstrate the reproducible nature of the RGO/SnO2 QD based sensor over passing time which shows excellent reproducibility.</P>
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Singh, Deepu,Sinha, B.,Rai, V.P.,Singh, M.N.,Singh, D.K.,Kumar, R.,Singh, A.K. The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.2
Inheritance of resistance to Fusarium wilt (FW) disease caused by Fusarium udum was investigated in pigeonpea using four different long duration FW resistant genotypes viz., BDN-2004-1, BDN-2001-9, BWR-133 and IPA-234. Based on the $F_2$ segregation pattern, FW resistance has been reported to be governed by one dominant gene in BDN-2004-1 and BDN-2001-9, two duplicate dominant genes in BWR-133 and two dominant complimentary genes in resistance source IPA-234. Further, the efficacy of six simple sequence repeat (SSR) markers namely, ASSR-1, ASSR-23, ASSR-148, ASSR-229, ASSR-363 and ASSR-366 reported to be associated with FW resistance were also tested and concluded that markers ASSR-1, ASSR-23, ASSR-148 will be used for screening of parental genotypes in pigeonpea FW resistance breeding programs. The information on genetics of FW resistance generated from this study would be used, to introgress FW resistance into susceptible but highly adopted cultivars through marker-assisted backcross breeding and in conventional breeding programs.
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