An In Vitro Study on Antimicrobial Activity of Propolis from Mugla Province of Turkey

Aysel Ugur,Tulin Arslan 한국식품영양과학회 2004 Journal of medicinal food Vol.7 No.1

Antibacterial and antifungal activities of acetone and dimethyl sulfoxide (DMSO) extracts of 45 different propo-lis samples from the Mugla province of Turkey were investigated. Antimicrobial activity of propolis varied depending onpropolis sample, dosage of propolis, and the extraction solvents for all test microorganisms. Antimicrobial activity of all propo-lis samples increased with increasing dosage without reaching a plateau at the highest dosage tested. Except for Brucellamelitensis,the DMSO extracts of all propolis samples were more active than the acetone extracts of the same samples. ForB. melitensis, the acetone extracts of all propolis samples showed greater activity. The most sensitive microorganism to propo-lis was Shigella sonnei in the gram-negative group and Streptococcus mutans in the gram-positive group. The least sensitivemicroorganism was Candida albicans.A control test run with standard antibiotics revealed that propolis samples from theMugla province of Turkey has a similar or greater inhibitory effect on S. mutans, Salmonella typhi, Pseudomonas aeruginosa,S. sonei, and C. albicans growth.

Chemical Composition of Endemic Scorzonera sandrasica and Studies on the Antimicrobial Activity Against Multiresistant Bacteria

Aysel Ugur,Nurdan Sarac,Ozgur Ceylan,M. Emin Duru,Yavuz Beyatli 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.3

The present study describes the antimicrobial activity and chemical composition of Scorzonera sandrasica Hartvig et Strid (Family Asteraceae), endemic to Turkey. The antimicrobial activity of the hexane, chloroform, ethyl acetate, and ethanol extracts of the aerial parts of S. sandrasica was evaluated against microorganisms, including multiresistant bacteria, using a paper disc diffusion method. The chemical composition of the chloroform extract of the plant was determined by gas chromatography and gas chromatography-mass spectrometry. The major compounds of the chloroform extract of the plant were caryophyllene oxide (19.7%), manoyl oxide (16.5%), and manool (11.3%), respectively. The extracts had antibacterial activity; however, no antifungal activity was observed against the two fungi. In particular, the ethanol and chloroform extracts exhibited significant activity against multiresistant strains of Stenotrophomonas maltophilia.

The In Vitro Antimicrobial Activities of the Essential Oils of Some Lamiaceae Species from Turkey

Sarac, Nurdan,Ugur, Aysel The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.4

In this study, antimicrobial activities of the essential oils obtained by using the hydrodistillation method from some lamiaceous plants-Mentha longifolia (L.) Hudson ssp. longifolia, M. longifolia (L.) Hudson ssp. typhoides (Briq.) Harley var. typhoides, Mentha pulegium L., Salvia fruticosa Miller, Salvia tomentosa Miller, Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P.W. Ball, Nepeta cadmea Boiss., Lavandula stoechas L. ssp. stoechas, and Ziziphora tenuior L.-were determined by using the disc diffusion method. The plants used in this study were collected from different localities of Mu$\breve{g}$la Province, Turkey. All the essential oils isolated from the plants were very effective against Gram-positive and Gram-negative bacteria, which included multiple-antibiotic resistant strains, except Pseudomonas aeruginosa ATCC 27853 and Pseudomonas fluorescens MU 87. The essential oils of the plant species, except S. tomentosa and S. fruticosa, were very effective against Candida albicans. The antimicrobial activities varied depending on the species, subspecies, or variety. In fact, essential oils of some plants belonging to the same taxa but collected from different localities showed different levels of antimicrobial activities.

The In Vitro Antimicrobial Activities of the Essential Oils of Some Lamiaceae Species from Turkey

Nurdan Sarac,Aysel Ugur 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.4

In this study, antimicrobial activities of the essential oils obtained by using the hydrodistillation method from some lamiaceous plants—Mentha longifolia (L.) Hudson ssp. longifolia, M. longifolia (L.) Hudson ssp. typhoides (Briq.) Harley var. typhoides, Mentha pulegium L., Salvia fruticosa Miller, Salvia tomentosa Miller, Calamintha nepeta (L.) Savi ssp. glandulosa (Req.) P.W. Ball, Nepeta cadmea Boiss., Lavandula stoechas L. ssp. stoechas, and Ziziphora tenuior L.—were determined by using the disc diffusion method. The plants used in this study were collected from different localities of Muğla Province, Turkey. All the essential oils isolated from the plants were very effective against Gram-positive and Gram-negative bacteria, which included multiple-antibiotic resistant strains, except Pseudomonas aeruginosa ATCC 27853 and Pseudomonas fluorescens MU 87. The essential oils of the plant species, except S. tomentosa and S. fruticosa, were very effective against Candida albicans. The antimicrobial activities varied depending on the species, subspecies, or variety. In fact, essential oils of some plants belonging to the same taxa but collected from different localities showed different levels of antimicrobial activities.

Antimicrobial Activities of the Essential Oils of Origanum onites L., Origanum vulgare L. Subspecies hirtum (Link) Ietswaart, Satureja thymbra L., and Thymus cilicicus Boiss. & Bal. Growing Wild in Turkey

Nurdan Sarac,Aysel Ugur 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.3

In the present study, four separate samples of Origanum onites L., three separate samples of Satureja thymbra L., Origanum vulgare L. ssp. hirtum (Link) Ietswaart, and Thymus cilicicus Boiss. & Bal. were collected from various regions of Mugla, Turkey. The essential oils of these plants were obtained by the hydrodistillation method. Antimicrobial activities of the essential oils against microorganisms, including multiple antibiotic-resistant bacteria, were investigated using the disc diffusion method. Different antibiotic discs were used for comparison to the inhibition zones. All the essential oils used in this study were very effective against Gram-positive and Gram-negative bacteria, which included multiple resistant strains, except Pseudomonas aeruginosa ATCC 27853 and Pseudomonas fluorescens MU 87. The essential oils of the O. onites, O. vulgare ssp. hirtum, and S. thymbra were especially very effective against the resistant strains such as Stenotrophomonas maltophilia MU 64, S. maltophilia MU 99, and Chryseomonas luteola MU 65. The maximum antimicrobial activity was observed with the essential oils of O. onites. The antimicrobial activities of the essential oils varied depending on the species, subspecies, or variety. In fact, the essential oils of some plants belonging to the same species that were collected from different locations showed different levels of antimicrobial activities.

Chemical composition and anti-biofilm activity of Thymus sipyleus BOISS. subsp. sipyleus BOISS. var. davisianus RONNIGER essential oil

Ozgur Ceylan,Aysel Ugur 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

In this study, antimicrobial and antibiofilmactivities and the chemical composition of Thymus sipyleusBOISS. subsp. sipyleus BOISS. var. davisianus RONNIGERessential oil was evaluated. The essential oil wasobtained by hydro-distillation and analyzed by gas chromatography-mass spectrometry. Fourteen compounds werecharacterized, having as major components thymol(38.31 %) and carvacrol (37.95 %). Minimum inhibitoryconcentrations (MICs) of oil and the major componentswere calculated by serial dilution method, and anti-biofilmeffects by microplate biofilm assay against five Grampositive (Staphylococcus aureus MU 38, MU 40, MU 46,MU 47, Stahylococcus epidermidis MU 30) and five Gramnegative (Pseudomonas aeruginosa MU 187, MU 188, MU189, Pseudomonas fluorescens MU 180, MU 181) bacteria. It was found that MICs for essential oil, thymol and carvacrolwere between 5 and 50 ll/ml, 0.125–0.5 lg/ml and0.125–05 ll/ml, respectively. The results showed thatdoses of MIC produced a greater anti-biofilm influencethan 0.5, 0.25 and 0.125 MIC. In the presence of essentialoil (MIC), the mean biofilm formation value was equal to67 ± 5.5 % for P. aeruginosa MU 188, and essential oil(MIC) inhibition exceeds 60 % for P. aeruginosa biofilms. The results also showed that carvacrol (MIC) was able toinduce an inhibition 72.9 ± 4.1 % for S.aureus (MU 40)biofilm. In addition, thymol (MIC) showed 68.6 ± 5.3 %reduction in biofilm formation of P. fluorescens MU 181. This study demonstrated the antimicrobial and antibiofilmactivity of T. sipyleus BOISS. subsp. sipyleus BOISS. var. davisianus RONNIGER essential oil and points out theexceptional efficiency of thymol and carvacrol, whichcould represent candidates in the treatment of Pseudomonasand Staphylococcus biofilms.