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        Impact of a long-lasting adult liquid diet on female reproductive performance in the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)

        Angelo Canale,Gabriele Gennari,Valeria Leoni,Russell H. Messing,Giovanni Benelli 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.2

        Researchers in many countries have devoted great effort to the development and validation of the sterile insect technique (SIT) against a number of tephritid species. To improve SIT cost-effectiveness and applicability against these serious economic pests, itwould be useful to improve currentmass-rearing procedures. While the benefits of using liquid larval diets have beenwidely tested, liquid diets for adult feeding have received far less attention. Here, we report on the impact of low-cost adult liquid diets with a range of sugar:protein ratios on female reproductive performance (i.e. pre-oviposition period, female egg load, larval–pupal developmental rate, adult emergence, sex ratio and longevity of offspring) in the Mediterranean fruit fly, Ceratitis capitata. Protein-deprived females required longer pre-oviposition time and had reduced egg load. No differences were found in the reproductive performance of females fed with liquid vs. solid diet. The proposed liquid diet is readily available to the feeding behaviour of tephritid flies and lasts three times longer than the solid diet composed of the same ingredients.

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        Contrasting genetic diversity and intra-population polymorphism of the invasive pest Henosepilachna vigintioctopunctata (Coleoptera, Coccinellidae): A DNA barcoding approach

        Pushparaj Karthika,Chithravel Vadivalagan,Natraj Krishnaveni,Kadarkarai MURUGAN,Marcello Nicoletti,Angelo Canale,Giovanni Benelli 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

        Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) is one of the most important pests attacking Solanaceae crops in Asian countries. Despite its agricultural importance, little information is available on the molecular ecology and evolution. In this study, we used DNA barcoding to explore the genetic diversity and intrapopulation polymorphisms of H. vigintioctopunctata. Haplotype analysis of the partial COI gene sequences of H. vigintioctopunctata fromfour localities in South India and other globally available haplotypes revealed two genetically distinctive lineages. The TCS haplotype network with the main clusters G1, G2, G4 and G6 represented intra-population polymorphismamong the Indian lineages,while other clusterswere differentiatedwith unique mutational steps. High substitutions in the 1st and 3rd codon suggested the dominance of synonymous substitutionswith significant p-values b 0.05, that inferred for purifying selection. The genetic diversity indices Fu's Fs and neutrality index Tajima-D had negative values that implied for recent population expansion and lowfrequency of polymorphism. Further, high genetic divergences among certain haplotype comparisons evidenced the influence of micro-evolutionary pressures. The threshold value was 1.26%, which was supported by the postulates of 10× rule. Overall, the study firstly reported the genetic diversity and intra-population polymorphism of H. vigintioctopunctata populations attacking Solanaceae species in Asian countries.

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