http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Ali Kowsari (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
-
Temperature profile for nanoscale Poiseuille flow: a multiscale study
Fahim Faraji,Ali Rajabpour,Farshad Kowsary 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.2
In this study, we calculated the temperature profile for a nanoscopic Poiseuille flow system via two methods. The first method involved employing the tools of Molecular dynamics (MD) simulation, and the second method involved solving Navier’s equation analytically while the rates of fluid dynamic viscosity and thermal conductivity were calculated through MD. We used a temperature jump model as the boundary condition required for the latter method, which was also calculated through MD. We repeated the calculations for various amounts of wall temperature and fluid-wall interaction strength, and we observed a satisfactory agreement between the results of the two methods.
-
Generation of Scalable Hepatic Micro-Tissues as a Platform for Toxicological Studies
Darakhshan Sara,Bidmeshki Pour Ali,Kowsari-Esfahan Reza,Vosough Massoud,Montazeri Leila,Ghanian Mohammad Hossein,Baharvand Hossein,Piryaei Abbas 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.4
Background: Currently, there is an urgent need for scalable and reliable in vitro models to assess the effects of therapeutic entities on the human liver. Hepatoma cell lines, including Huh-7, show weakly resemblance to human hepatocytes, limiting their significance in toxicity studies. Co-culture of hepatic cells with non-parenchymal cells, and the presence of extracellular matrix have been shown to influence the biological behavior of hepatocytes. The aim of this study was to generate the scalable and functional hepatic micro-tissues (HMTs). Methods: The size-controllable HMTs were generated through co-culturing of Huh-7 cells by mesenchymal stem cells and human umbilical vein endothelial cells in a composite hydrogel of liver-derived extracellular matrix and alginate, using an air-driven droplet generator. Results: The generated HMTs were functional throughout a culture period of 28 days, as assessed by monitoring glycogen storage, uptake of low-density lipoprotein and indocyanine green. The HMTs also showed increased secretion levels of albumin, alpha-1-antitrypsin, and fibrinogen, and production of urea. Evaluating the expression of genes involved in hepatic-specific and drug metabolism functions indicated a significant improvement in HMTs compared to two-dimensional (2D) culture of Huh-7 cells. Moreover, in drug testing assessments, HMTs showed higher sensitivity to hepatotoxins compared to 2D cultured Huh-7 cells. Furthermore, induction and inhibition potency of cytochrome P450 enzymes confirmed that the HMTs can be used for in vitro drug screening. Conclusion: Overall, we developed a simple and scalable method for generation of liver micro-tissues, using Huh-7, with improved hepatic-specific functionality, which may represent a biologically relevant platform for drug studies.
-
Seyedeh Saeideh Sahraei,Ali Kowsari,Faezeh Davoodi Asl,Mohsen Sheykhhasan,Leila Naserpoor,Azar Sheikholeslami 대한해부학회 2022 Anatomy & Cell Biology Vol.55 No.1
Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium (CM) from menstrual blood-derived mesenchymal stem cells (MenSCs) of healthy women can affect the expression level of inf lammatory and stemness genes of MenSCs from endometriosis women. Endometriosis-derived MenSCs (E-MenSCs) were treated with CM derived from healthy women’s MenSCs (non-endometriosis derived MenSCs [NE-MenSCs]). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the interleukin-1, cyclooxygenase-2, and hypoxia-inducible factor 1α as inflamaturay genes and octamer-binding transcription factor 4, NANOG, and sex determining region Y-box 2 as stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Our study indicates that the expression level of some inflammatory- and stemness-related genes which have differential expression in E-MenSCs compared with NE-MenSCs, could be changed to normal status by using CM derived from NE-MenSCs.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
