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        Artificial diet development and its effect on the reproductive performances of Propylea japonica and Harmonia axyridis

        Intazar Ali,Shuai Zhang,Jun-Yu Luo,Chun-Yi Wang,Li-Min Lv,Jin-Jie Cui 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2

        The use of augmentative biological controls in greenhouse pest management has increased the demand for predators. To meet demands, suitable artificial diets must be developed to enable fast and cost-effective mass rearing of predators. In this study, three artificial diets were developed and their suitability for immature development and survival of Japanese ladybird Propylea japonica (Thunberg) and Multi-colored Asian ladybird Harmonia axyridis (Pallas) were investigated. An artificial diet composed of shrimp, beef, beef liver, and egg yolk in a 5:4:8:4 ratio is an adequate source of nutrition for the development of immature P. japonica and H. axyridis, resulting in 83% and 68% adult emergence rates, respectively. The reproductive performances of emerging P. japonica and H. axyridis females fed the artificial diet or pea aphidswere compared. The pre-oviposition period was shorter in emerging females from immature ladybirds fed an artificial diet compared with those emerging from immature ladybirds fed pea aphids. However, the total fecundity did not vary significantly. When adults were fed artificial diets, females did not lay eggs, but oviposition could be restored by feeding adults on pea aphids. The developed artificial diet can be used for the mass rearing of P. japonica and H. axyridis for biological control applications and rearing of adults during prey shortage.

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        Comparative effectiveness of wild bee pollination on the post-harvest characteristics of Grewia asiatica (Malvaceae)

        Akram Waseem,Sajjad Asif,Ali Mudssar,Ahmad Ammad,Ali Intazar,Saddiq Bushra,Yasin Muhammad,Anjum Aqueel Muhammad 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3

        Measuring the pollination potential of different native bees is helpful in identifying the most efficient species for their conservation and proper utilization. The current study was carried out at the Research Farm of The Islamia University of Bahawalpur to compare the pollination efficiency of four native bee species: Apis dorsata, Megachile bicolor, M. cephalotes and Megachile (Callomegachile) sp. Apis dorsata was the most abundant floral visitor of Grewia asiatica comprising 36.46% of floral visitors following by M. cephalotes and Megachile sp. The peak abundance of A. dorsata and M. bicolor was recorded at 12:00 and 08:00, respectively whereas for M. cephalotes and Megachile sp. at 16:00. Visitation frequency, stay time, body and proboscis length were significantly higher in A. dorsata whereas visitation rate, pollen load and pollen deposition were significantly higher in M. bicolor. The maximum fruit weight was recorded when M. bicolor and Megachile sp. had been the pollinators. The post-harvest fruit weight loss was lower in M. bicolor pollinated fruit -with more gradual wrinkling- than in fruit that had resulted from pollination by A. dorsata, M. cephalotes and Megachile sp. Our results suggest that M. bicolor was better pollinator of G. asiatica in terms of its reproductive success and post-harvest qualities. Further studies should focus on conservation of M. bicolor in the region.

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        Aloe vera gel enriched with garlic essential oil eff ectively controls anthracnose disease and maintains postharvest quality of banana

        Ghulam Khaliq,Hafi z Tassawar Abbas,Intazar Ali,Muhammad Waseem 한국원예학회 2019 Horticulture, Environment, and Biotechnology Vol.60 No.5

        Banana is a tropical fruit that suff ers from several postharvest diseases during transportation and storage. Anthracnosecaused by the fungus Colletotrichum musae is the most destructive postharvest disease of banana. The aim of this studywas to determine the antifungal activities of Aloe vera (AV) gel coating alone or in combination with garlic oil (GO) at twoconcentrations (AV + GO 0.05% and AV + GO 0.1%) in vitro and in vivo against anthracnose disease of banana fruit. Theresults showed that the AV gel coating incorporated with GO was more eff ective as a fungicide than AV gel alone. The highestantifungal activity was observed in AV + GO 0.1% treatment, which signifi cantly inhibited the mycelial growth and sporegermination by 87.7 and 91.2%, respectively, compared to the control. In vivo study indicated that AV gel combined withGO 0.1% eff ectively reduced anthracnose disease incidence (92.5%) and severity (81.0%) in artifi cially inoculated bananafruit after 15 days of storage. The same treatment delayed the changes in weight loss, fi rmness, soluble solids concentration,and titratable acidity. Moreover, AV gel coating and GO enhanced total phenolic contents and total antioxidant activities ofbanana fruit. These results suggested that AV gel combined with GO can be used as an eff ective biofungicide for controllinganthracnose disease of banana fruit.

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