Rosmarinic Acid Protects Adipose Tissue-Derived Mesenchymal Stem Cells in Nutrient-Deficient Conditions.

Ahmad Ghorbani,Hamid Reza Sadeghnia,Amir Reza Afshari,Azar Hosseini 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.4

One of the major challenges for stem cell therapy of ischemic organs is that the transplanted cells are confronted with nutrient deficiency and oxidative stress. Previous studies have indicated that pretreatment of stem cells with cytoprotective phytochemicals improves their therapeutic potential. This study was aimed to investigate whether rosmarinic acid can enhance survival of adipose tissue-derived stem cells (ASCs) in nutrient-deficient culture as an in vitro model of ischemia. The ASCs were isolated from subcutaneous adipose tissue of male adult Wistar rats and incubated for 24 h with rosmarinic acid in nutrient-deficient (glucose- and serum-deprived, GSD) culture medium. In a separate experiment, ASCs were pre-incubated for 4 h with rosmarinic acid and then exposed to GSD conditions for 24 h. The viability of ASCs was determined using thiazolyl blue tetrazolium bromide assays. The effect of rosmarinic acid on the cell cycle was evaluated using propidium iodide staining. GSD conditions significantly decreased the viability of ASCs and enhanced the generation of reactive oxygen species (ROS), lipid peroxidation, sub-G1 cell populations, and necrosis. Both pre-incubation and incubation of ASCs with 0.75∼6 M rosmarinic acid significantly increased cell viability in GSD conditions. Rosmarinic acid further decreased the level of ROS, lipid peroxidation, the percent of cells in sub-G1 stage, and necrosis in GSD conditions. These findings suggest that rosmarinic acid enhances survival of ASCs cultured in nutrient-deficient conditions through promoting antioxidant effects. Therefore, rosmarinic acid may help preserve ASCs survival after they are transplanted into ischemic organs.

The simultaneous adsorption and desorption of flavonoids from bitter orange peel by the carboxylated multi-walled carbon nanotubes

Gholizadeh Hassan,Ghorbani-HasanSaraei Azade,Tahermansouri Hasan,Shahidi Seyed-Ahmad 한국탄소학회 2019 Carbon Letters Vol.29 No.3

The carboxylated multi-walled carbon nanotubes (MWCNTs–COOH) were used as adsorbent for the separation of flavonoids (naringin and rutin) from bitter orange peel. The influence of the parameters such as, pH values, contact time, and desorption conditions was investigated. The samples were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, derivative thermogravimetric, scanning electron microscopy, UV–Vis spectroscopy, and high-performance liquid chromatography. After separation and desorption process, the eluent was injected for chromatography analysis. Under the optimal conditions, experimental results showed that the extraction efficiency of rutin was higher than naringin and other compounds. Moreover, the desorption percentage of flavonoids was calculated 83.6% after four cycles. This research confirmed that this method for separation of flavonoids is simple and less cost. In addition, the separated flavonoids can be used as antioxidant for the future applications.

The simultaneous adsorption and desorption of flavonoids from bitter orange peel by the carboxylated multi‑walled carbon nanotubes

Hassan Gholizadeh,Azade Ghorbani‑HasanSaraei,Hasan Tahermansouri,Seyed‑Ahmad Shahidi 한국탄소학회 2019 Carbon Letters Vol.29 No.3

The carboxylated multi-walled carbon nanotubes (MWCNTs–COOH) were used as adsorbent for the separation of flavonoids (naringin and rutin) from bitter orange peel. The influence of the parameters such as, pH values, contact time, and desorption conditions was investigated. The samples were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, derivative thermogravimetric, scanning electron microscopy, UV–Vis spectroscopy, and high-performance liquid chromatography. After separation and desorption process, the eluent was injected for chromatography analysis. Under the optimal conditions, experimental results showed that the extraction efficiency of rutin was higher than naringin and other compounds. Moreover, the desorption percentage of flavonoids was calculated 83.6% after four cycles. This research confirmed that this method for separation of flavonoids is simple and less cost. In addition, the separated flavonoids can be used as antioxidant for the future applications.

Effects of standardized extracts of Lamium album and Urtica dioica on rat tracheal smooth muscle contraction

Arefani, Samane,Mehran, Seyyed Mohammad Mohseni,Moladoust, Hassan,Norasfard, Mohammad Reza,Ghorbani, Ahmad,Abedinzade, Mahmood KOREAN PHARMACOPUNCTURE INSTITUTE 2018 Journal of pharmacopuncture Vol.21 No.2

Objective: Diseases of the respiratory system are one of the main causes of death and include situation such as chronic obstructive pulmonary disease, pneumonia or asthma. Medicinal plants have beneficial effects on multiple diseases include respiratory disorders like asthma and bronchitis. The aim of this study was to evaluate the effects of U. dioica and L. Album on tracheal smooth muscle contraction. Material and methods: Hydroalcoholic extracts of L. Album and U. Dioica aerial parts were prepared by maceration method and standardized based on their total phenol content. The effect of the extracts on the KCl-induced contraction of rat trachea was examined in an organ bath chamber. Data was analyzed with spss software 22. Results: The extract of L. Album (5 mg/ml), similar to theophylline (20 mM), significantly reduced the KCl-induced tracheal contraction. On the other hand, U. Dioica (1 mg/ml and 5 mg/ml) augmented the KCl-induced contraction. Conclusion: The relaxant effect of L. Album on the trachea makes it as a candidate for the managing patients with asthma and obstructive pulmonary diseases. But because of U. Dioica potential constrictive effect on the trachea it is suggested that patients avoid consuming it.

Effects of standardized extracts of Lamium album and Urtica dioica on rat tracheal smooth muscle contraction

Samane Arefani,Seyyed Mohammad Mohseni Mehran,Hassan Moladoust,Mohammad Reza Norasfard,Ahmad Ghorbani,Mahmood Abedinzade 대한약침학회 2018 Journal of pharmacopuncture Vol.21 No.2

Objective: Diseases of the respiratory system are one of the main causes of death and include situation such as chronic obstructive pulmonary disease, pneumonia or asthma. Medicinal plants have beneficial effects on multiple diseases include respiratory disorders like asthma and bronchitis. The aim of this study was to evaluate the effects of U. dioica and L. Album on tracheal smooth muscle contraction. Material and methods: Hydroalcoholic extracts of L. Album and U. Dioica aerial parts were prepared by maceration method and standardized based on their total phenol content. The effect of the extracts on the KCl-induced contraction of rat trachea was examined in an organ bath chamber. Data was analyzed with spss software 22. Results: The extract of L. Album (5 mg/ml), similar to theophylline (20 mM), significantly reduced the KCl-induced tracheal contraction. On the other hand, U. Dioica (1 mg/ml and 5 mg/ml) augmented the KCl-induced contraction. Conclusion: The relaxant effect of L. Album on the trachea makes it as a candidate for the managing patients with asthma and obstructive pulmonary diseases. But because of U. Dioica potential constrictive effect on the trachea it is suggested that patients avoid consuming it.

A promising wound dressing based on alginate hydrogels containing vitamin D3 cross‑linked by calcium carbonate/d‑glucono‑δ‑lactone

Arian Ehterami,Majid Salehi,Saeed Farzamfar,Hadi Samadian,Ahmad Vaez,Hamed Sahrapeyma,Sadegh Ghorbani 대한의용생체공학회 2020 Biomedical Engineering Letters (BMEL) Vol.10 No.2

In the present study, we fabricated vitamin D 3 -loaded alginate hydrogel and assessed its wound healing capability in the animalmodel. The various concentrations of vitamin D3 were added to the pre-dissolved sodium alginate in deionized water andcross-linked by calcium carbonate in combination with D -glucono-δ-lactone. The microstructure, swelling behavior, weightloss, hemo- and cytocompatibility of the fabricated hydrogels were evaluated. In the last stage, the therapeutic effi cacy ofthe prepared hydrogels was evaluated in the full-thickness dermal wound model. The scanning electron microscopy imagesshowed that the prepared hydrogel was highly porous with the porosity of 89.2 ± 12.5% and contained the interconnectedpores. Weight loss assessment showed that the prepared hydrogel is biodegradable with the weight loss percentage of about89% in 14 days. The results showed that the prepared hydrogels were hemo- and cytocompatible. The animal study resultsimplied that alginate hydrogel/3000 IU vitamin D 3 group exhibited the highest wound closure present which was statisticallysignifi cant than the control group ( p < 0.05). Moreover, the histological examinations revealed that hydrogel containing3000 IU vitamin D3 had the best performance and induced the highest re-epithelialization and granular tissue formation. Allin all, this study suggests that alginate hydrogels with 3000 IU vitamin D 3 can be exploited as a potential wound dressingin skin tissue engineering.

Evaluation Potential Antidiabetic Effects of Ferula latisecta in Streptozotocin-Induced Diabetic Rats

Javanshir, Sajad,Soukhtanloo, Mohammad,Jalili-Nik, Mohammad,Yazdi, Amirali Jahani,Amiri, Mohammad Sadegh,Ghorbani, Ahmad KOREAN PHARMACOPUNCTURE INSTITUTE 2020 Journal of pharmacopuncture Vol.23 No.3

Objectives: The aim of the present work was to evaluate the possible beneficial effects of F. latisecta on blood glucose, lipids, and diabetes-related changes in the liver and kidney of streptozotocin-induced diabetic rats. Methods: Male Wistar rats were randomly allocated into four groups (n = 6): normal control rats, diabetic control rats, diabetic rats treated for 4 weeks with F. latisecta root (400 mg/kg/day), and diabetic rats treated with F. latisecta aerial parts (400 mg/kg/day). Results: Induction of diabetes significantly (p < 0.05) increased the levels of fasting blood glucose (FBG), triglyceride, total cholesterol, low-density lipoprotein (LDL), blood urea nitrogen (BUN), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). Diabetes also increased (p < 0.05) oxidative stress in the kidney and liver (decrease of thiol and increase of superoxide dismutase). The root and aerial parts of F. latisecta significantly reduced the level of LDL (p < 0.05) and restored the content of thiol (p < 0.05) and superoxide dismutase (p < 0.01) in the kidney and liver. F. latisecta had no significant effect on the levels of FBG, BUN, AST, and ALT. The root of F. latisecta also reduced the serum level of total cholesterol (p < 0.05) and prevented the progression of hyperglycemia. Conclusion: These findings suggest that F. latisecta may improve diabetic dyslipidemia by reducing serum LDL. Further studies are needed to confirm our findings.