PLGA/nHA hybrid nanofiber scaffold as a nanocargo carrier of insulin for accelerating bone tissue regeneration

Haider, Adnan,Gupta, Kailash Chandra,Kang, Inn-Kyu Springer 2014 NANOSCALE RESEARCH LETTERS Vol.9 No.1

<P>The development of tissue engineering in the field of orthopedic surgery is booming. Two fields of research in particular have emerged: approaches for tailoring the surface properties of implantable materials with osteoinductive factors as well as evaluation of the response of osteogenic cells to these fabricated implanted materials (hybrid material). In the present study, we chemically grafted insulin onto the surface of hydroxyapatite nanorods (nHA). The insulin-grafted nHAs (nHA-I) were dispersed into poly(lactide-co-glycolide) (PLGA) polymer solution, which was electrospun to prepare PLGA/nHA-I composite nanofiber scaffolds. The morphology of the electrospun nanofiber scaffolds was assessed by field emission scanning electron microscopy (FESEM). After extensive characterization of the PLGA/nHA-I and PLGA/nHA composite nanofiber scaffolds by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), X-ray photoelectron spectroscopy (XPS), energy-dispersive X-ray spectrometry (EDS), and transmission electron microscopy (TEM), the PLGA/nHA-I and PLGA/nHA (used as control) composite nanofiber scaffolds were subjected to cell studies. The results obtained from cell adhesion, alizarin red staining, and Von Kossa assay suggested that the PLGA/nHA-I composite nanofiber scaffold has enhanced osteoblastic cell growth, as more cells were proliferated and differentiated. The fact that insulin enhanced osteoblastic cell proliferation will open new possibilities for the development of artificial scaffolds for bone tissue regeneration.</P>

Preparation of Silver Nanoparticles and Their Industrial and Biomedical Applications: A Comprehensive Review

Haider, Adnan,Kang, Inn-Kyu Hindawi Limited 2015 Advances In Materials Science And Engineering Vol.2015 No.-

<P>Silver nanoparticles (Ag-NPs) have diverted the attention of the scientific community and industrialist itself due to their wide range of applications in industry for the preparation of consumer products and highly accepted application in biomedical fields (especially their efficacy against microbes, anti-inflammatory effects, and wound healing ability). The governing factor for their potent efficacy against microbes is considered to be the various mechanisms enabling it to prevent microbial proliferation and their infections. Furthermore a number of new techniques have been developed to synthesize Ag-NPs with controlled size and geometry. In this review, various synthetic routes adapted for the preparation of the Ag-NPs, the mechanisms involved in its antimicrobial activity, its importance/application in commercial as well as biomedical fields, and possible application in future have been discussed in detail.</P>

Analysis of Meteorological factors on Groundwater Level variations using Long Short-Term Memory Network

Adnan Haider(어드난 하이더),Pilsun Yoon(윤필선),Heedong Park(박희동),Kyoungson Jhang(장경선) 한국정보과학회 2022 한국정보과학회 학술발표논문집 Vol.2022 No.12

Morphological Effects of HA on the Cell Compatibility of Electrospun HA/PLGA Composite Nanofiber Scaffolds

Haider, Adnan,Gupta, Kailash Chandra,Kang, Inn-Kyu Hindawi Publishing Corporation 2014 BioMed research international Vol.2014 No.-

<P>Tissue engineering is faced with an uphill challenge to design a platform with appropriate topography and suitable surface chemistry, which could encourage desired cellular activities and guide bone tissue regeneration. To develop such scaffolds, composite nanofiber scaffolds of nHA and sHA with PLGA were fabricated using electrospinning technique. nHA was synthesized using precipitation method, whereas sHA was purchased. The nHA and sHA were suspended in PLGA solution separately and electrospun at optimized electrospinning parameters. The composite nanofiber scaffolds were characterized by FE-SEM, EDX analysis, TEM, XRD analysis, FTIR, and X-ray photoelectron. The potential of the HA/PLGA composite nanofiber as bone scaffolds in terms of their bioactivity and biocompatibility was assessed by culturing the osteoblastic cells onto the composite nanofiber scaffolds. The results from <I>in vitro </I>studies revealed that the nHA/PLGA composite nanofiber scaffolds showed higher cellular adhesion, proliferation, and enhanced osteogenesis performance, along with increased Ca<SUP>+2</SUP> ions release compared to the sHA/PLGA composite nanofiber scaffolds and pristine PLGA nanofiber scaffold. The results show that the structural dependent property of HA might affect its potential as bone scaffold and implantable materials in regenerative medicine and clinical tissue engineering.</P>

Antibacterial Activity and Cytocompatibility of PLGA/CuO Hybrid Nanofiber Scaffolds Prepared by Electrospinning

Haider, Adnan,Kwak, Sanghwa,Gupta, Kailash Chandra,Kang, Inn-Kyu Hindawi Limited 2015 Journal of nanomaterials Vol.2015 No.-

<P>The PLGA/CuO hybrid nanofibers scaffolds were prepared via electrospinning technique. The presence of CuO in the PLGA scaffolds was confirmed by transmission electron microscope (TEM) and X-ray photoelectron spectroscopy (XPS). The scaffolds were subjected to various antibacterial and cytobiocompatibility tests. The results not only showed excellent adhesion, proliferation, and viability (live/dead staining) for fibroblastic cells but also revealed that PLGA/CuO hybrid nanofiber scaffolds inhibited both Gram-positive and Gram-negative bacterial growth. The mechanism of the antibacterial activity was concluded to be based on the CuO nanoparticles and Cu<SUP>++</SUP>ions release. It is, therefore, evaluated that PLGA/CuO hybrid nanofiber scaffolds can be a useful candidate for wound dressing.</P>

Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering

Kwak, Sanghwa,Haider, Adnan,Gupta, Kailash Chandra,Kim, Sukyoung,Kang, Inn-Kyu Springer US 2016 NANOSCALE RESEARCH LETTERS Vol.11 No.1

<P>The dual extrusion electrospinning technique was used to fabricate multilayered 3D scaffolds by stacking microfibrous meshes of poly(lactic acid-co-glycolic acid) (PLGA) in alternate fashion to micro/nano mixed fibrous meshes of PLGA and collagen. To fabricate the multilayered scaffold, 35 wt% solution of PLGA in THF-DMF binary solvent (3:1) and 5 wt% solution of collagen in hexafluoroisopropanol (HFIP) with and without hydroxyapatite nanorods (nHA) were used. The dual and individual electrospinning of PLGA and collagen were carried out at flow rates of 1.0 and 0.5 mL/h, respectively, at an applied voltage of 20 kV. The density of collagen fibers in multilayered scaffolds has controlled the adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells. The homogeneous dispersion of glutamic acid-modified hydroxyapatite nanorods (nHA-GA) in collagen solution has improved the osteogenic properties of fabricated multilayered scaffolds. The fabricated multilayered scaffolds were characterized using FT-IR, X-ray photoelectron spectroscopy, and transmission electron microscopy (TEM). The scanning electron microscopy (FE-SEM) was used to evaluate the adhesion and spreads of MC3T3-E1 cells on multilayered scaffolds. The activity of MC3T3-E1 cells on the multilayered scaffolds was evaluated by applying MTT, alkaline phosphatase, Alizarin Red, von Kossa, and cytoskeleton F-actin assaying protocols. The micro/nano fibrous PLGA-Col-HA scaffolds were found to be highly bioactive in comparison to pristine microfibrous PLGA and micro/nano mixed fibrous PLGA and Col scaffolds.</P>

Photoinduced Development of Antibacterial Materials Derived from Isosorbide Moiety

Lorenzini, Cedric,Haider, Adnan,Kang, Inn-Kyu,Sangermano, Marco,Abbad-Andalloussi, Samir,Mazeran, Pierre-Emmanuel,Lalevé,e, Jacques,Renard, Estelle,Langlois, Valé,rie,Versace, Davy-Louis American Chemical Society 2015 Biomacromolecules Vol.16 No.3

<P>A straightforward method for immobilizing in situ generated silver nanoparticles on the surface of a photoactivable isosorbide-derived monomer is developed with the objective to design a functional material having antibacterial properties. The photoinduced thiol–ene mechanism involved in these syntheses is described by the electron spin resonance/spin trapping technique. The resulting materials with or without silver nanoparticles (Ag NPs) were used as films or as coatings on glass substrate. The surface of the synthesized materials was characterized by X-ray photoelectron spectroscopy and scanning electron microscopy, and their thermal and mechanical properties were evaluated by dynamic-mechanical thermal tests, differential scanning calorimetry, thermogravimetric analyses, along with pencil hardness, nanoindentation, and scratch resistance tests. The photoinduced formation of Ag NPs is also confirmed by UV spectrophotometry. Finally, a primary investigation demonstrates the antibacterial properties of the isosorbide-derived material against <I>Staphylococcus aureus</I> and <I>Escherichia coli</I>, as well as its cytocompatibility toward NIH 3T3 fibroblastic cells.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bomaf6/2015/bomaf6.2015.16.issue-3/bm501755r/production/images/medium/bm-2014-01755r_0016.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bm501755r'>ACS Electronic Supporting Info</A></P>

Synthesis and characterization of graphene oxide, reduced graphene oxide and their nanocomposites with polyethylene oxide

Alghyamah Abdulaziz A.,Haider Sajjad,Khalil Uzma,Khan Rawaiz,Haider Adnan,Almasry Waheed A.,Ihsan Rida,Tahira Perveen,Wazeer Irfan,Chafidz Achmad 한국물리학회 2022 Current Applied Physics Vol.40 No.-

This work describes the synthesis of GO, rGO and their nanocomposites with PEO. GO and rGO were prepared by the modified Hummers method and in-situ reduction of GO utilizing green reductant L (+) Ascorbic acid. The nanocomposites were characterized by Fourier-transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD), Field emission scanning electron microscopy (FE-SEM), Thermogravimetric Analysis (TGA), and Universal Testing Machine (UTM). FT-IR and XRD confirmed the synthesis of GO and rGO. FE-SEM confirmed the uniformly exfoliated GO and rGO nanosheets in the polymer matrix. Hydrogen bonding was the main interaction mechanism for GO with PEO while no interaction was detected by FT-IR for rGO. Enhanced thermal stability was observed for both GO/PEO and rGO/PEO nanocomposites. The mechanical analysis showed an increase in Young’s modulus, tensile strength, and elongation at break for GO/PEO nanocomposites, which is attributed to the homogeneous dispersion and hydrophilic hydrogen bonding interaction of GO with PEO.

Nanofibrous Scaffolds in Biomedical Applications

Kailash Chandra Gupta,Adnan Haider,최유리,강인규 한국생체재료학회 2014 생체재료학회지 Vol.18 No.2

Nanofibrous scaffolds are artificial extracellular matrices which provide natural environment for tissue formation. In comparison to other forms of scaffolds, the nanofibrous scaffolds promote cell adhesion, proliferation and differentiation more efficiently due to having high surface to volume ratio. Although scaffolds for tissue engineering have been fabricated by various techniques but electrospun nanofibrous scaffolds have shown great potential in the fields of tissue engineering and regeneration. This review highlights the applications and importance of electrospun nanofibrous scaffolds in various fields of biomedical applications ranging from drug delivery to wound healing. Attempts have also been made to highlights the advantages and disadvantages of nanofirbous scaffolds fabricated for biomedical applications using technique of electrospinning. The role of various factors controlling drug distribution in electrospun nanofibrous scaffolds is also discussed to increase the therapeutic efficiency of nanofibrous scaffolds in wound healing and drug delivery applications.

Fabrication of highly porous PMMA electrospun fibers and their application in the removal of phenol and iodine

Bae, Hyun-Su,Haider, Adnan,Selim, K. M. Kamruzzaman,Kang, Dong-Yoon,Kim, Eun-Jin,Kang, Inn-Kyu Springer-Verlag 2013 Journal of polymer research Vol.20 No.7