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ALCAM is a Novel Cytoplasmic Membrane Protein in TNF-α Stimulated Invasive Cholangiocarcinoma Cells
Adisakwattana, Poom,Suwandittakul, Nantana,Petmitr, Songsak,Wongkham, Sopit,Sangvanich, Polkit,Reamtong, Onrapak Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Background: Cholangiocarcinoma (CCA), or bile duct cancer, is incurable with a high mortality rate due to a lack of effective early diagnosis and treatment. Identifying cytoplasmic membrane proteins of invasive CCA that facilitate cancer progression would contribute toward the development of novel tumor markers and effective chemotherapy. Materials and Methods: An invasive CCA cell line (KKU-100) was stimulated using TNF-${\alpha}$ and then biotinylated and purified for mass spectrometry analysis. Novel proteins expressed were selected and their mRNAs expression levels were determined by real-time RT-PCR. In addition, the expression of ALCAM was selected for further observation by Western blot analysis, immunofluorescent imaging, and antibody neutralization assay. Results: After comparing the proteomics profile of TNF-${\alpha}$ induced invasive with non-treated control cells, over-expression of seven novel proteins was observed in the cytoplasmic membrane of TNF-${\alpha}$ stimulated CCA cells. Among these, ALCAM is a novel candidate which showed significant higher mRNA- and protein levels. Immunofluorescent assay also supported that ALCAM was expressed on the cell membrane of the cancer, with increasing intensity associated with TNF-${\alpha}$. Conclusions: This study indicated that ALCAM may be a novel protein candidate expressed on cytoplasmic membranes of invasive CCA cells that could be used as a biomarker for development of diagnosis, prognosis, and drug or antibody-based targeted therapies in the future.
Synthesis of N-phenylphthalimide Derivatives as ${\alpha}-Glucosidase$ Inhibitors
Pluempanupat, Wanchai,Adisakwattana, Sirichai,Yibchok-Anun, Sirintorn,Chavasiri, Warinthorn 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.12
Sixteen N-phenylphthalimide derivatives were synthesized and their ability to inhibit ${\alpha}-glucosidase$ was investigated. N-(2,4-dinitrophenyl)phthalimide was a potent inhibitor of yeast ${\alpha}-glucosidase\;(IC_{50};\;0.158{\pm}0.005mM)\;and\;maltase\;(IC_{50};\;0.051{\pm}0.008mM)$, whereas it did not inhibit sucrase. From a Lineweaver-Burk plot of ${\alpha}-glucosidase$ kinetics, N-(2,4-dichlorophenyl)phthalimide was found to be a competitive inhibitor of yeast ${\alpha}-glucosidase$. These results indicate that N-(2,4-dinitrophenyl)phthalimide could be a representative of a new group of ${\alpha}-glucosidase$ inhibitors.
Synthesis of N-phenylphthalimide Derivatives as α-Glucosidase Inhibitors
Wanchai Pluempanupat,Sirichai Adisakwattana,Sirintorn Yibchok-Anun,Warinthorn Chavasiri 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.12
Sixteen N-phenylphthalimide derivatives were synthesized and their ability to inhibit α-glucosidase was investigated. N-(2,4-dinitrophenyl)phthalimide was a potent inhibitor of yeast α-glucosidase (IC50; 0.158 ± 0.005 mM) and maltase (IC50; 0.051 ± 0.008 mM), whereas it did not inhibit sucrase. From a Lineweaver-Burk plot of α-glucosidase kinetics, N-(2,4-dichlorophenyl) phthalimide was found to be a competitive inhibitor of yeast α-glucosidase. These results indicate that N-(2,4-dinitrophenyl)phthalimide could be a representative of a new group of α- glucosidase inhibitors.
Pasukamonset, Porntip,Kwon, Oran,Adisakwattana, Sirichai Elsevier 2016 Food hydrocolloids Vol.61 No.-
<P><B>Abstract</B></P> <P>Microencapsulation of phenolic extracts of <I>Clitoria ternatea</I> (CT) petal flower extract through extrusion method of alginate with calcium chloride (CaCl<SUB>2</SUB>) was studied. Encapsulation efficiency varied in the range from 74.17 ± 0.83% to 84.87 ± 0.29% depending on the percentage of CT (5–20%), alginate (1–2%), and CaCl<SUB>2</SUB> (1.5–5%). The results showed that the optimized condition of CT-loaded alginate beads (CT beads) was as follows: 10% CT, 1.5% alginate, and 3% CaCl<SUB>2</SUB> (w/v). Under this condition, the maximal antioxidant capacity of 11.76 ± 0.07 mg gallic acid equivalent/g<SUB>beads</SUB> and the encapsulation efficiency of 84.83 ± 0.40% were obtained. The microencapsulation was found to have smooth surface shape with a particle size distribution of 985 ± 0.53 μm and improve the thermal stability with 188 °C. There was the absence of chemical interactions between CT and alginate as verified by using FT-IR. The microencapsulation of CT significantly retains higher amount of polyphenols and improves antioxidant capacity, pancreatic α-amylase inhibitory activity, and bile acid binding after the gastrointestinal digestion. This study provides a novel food-grade encapsulation formulation to improve the stability as well as the biological activity of plant polyphenols.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CT was encapsulated in calcium alginate beads. </LI> <LI> Encapsulated CT retained polyphenols and antioxidant activity. </LI> <LI> Encapsulating CT increased pancreatic α-amylase inhibitory activity after digestion. </LI> <LI> Encapsulating CT increased bile acid binding after digestion. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Park, Soo-yeon,Jin, Bora,Shin, Jae-Ho,Adisakwattana, Sirichai,Kwon, Oran EDITIONS SCIENTIFIQUES ELSEVIER 2017 BIOMEDICINE AND PHARMACOTHERAPY Vol.92 No.-
<P><B>Abstract</B></P> <P>Abnormalities in the hyperbolic relationship between insulin sensitivity and insulin secretion may cause oxidative stress and non-enzymatic glycation, resulting in an increased risk of type 2 diabetes. Here, we performed a 14-week study to investigate the effects of ethanolic extract of <I>Mori ramulus</I> (MRE; 0, 800, and 1600mg/kg body weight) and its signature component oxyresveratrol (OXY; 800mg/kg body weight) on β-cell dysfunction and insulin resistance in C57BLKS/J db/db mice fed with a high-fat diet. Compared with the diabetic control group, the high-dose MRE group showed a significant decrease in fasting blood glucose (<I>p</I> =0.0024); a significant increase in insulin secretion as measured by insulin (<I>p</I> =0.0012) and C-peptide (<I>p</I> =0.0103) levels in plasma and insulin content (<I>p</I> =0.0440) and homeobox factor-1 protein expression (<I>p</I> =0.0148) in the pancreas; and a significant increase in insulin sensitivity as measured by insulin receptor mRNA expression in the liver (<I>p</I> =0.0179) and adipose tissue (<I>p</I> =0.0491). In addition, improvements in the reactive oxygen species level and inflammatory pancreatic and hepatic tissue damage were also observed in the MRE group as assessed by histological findings. A similar but weaker effect was found in the OXY group. Furthermore, we observed a potentiating effect of MRE and OXY on insulin secretion in INS-1 cells in the presence of 27mM glucose, together with an anti-glycation effect as indicated by methylglyoxal-trapping capacity and inhibition of advanced glycation end-product formation. Taken together, these data suggest that MRE could ameliorate β-cell dysfunction and insulin resistance by reducing oxidative damage and advanced glycation end-product (Wagenknecht et al., 2003) formation and that these effects are due, at least in part, to OXY.</P>
Amornrat Geadkaew-Krenc,Rudi Grams,Wansika Phadungsil,Wanlapa Chaibangyang,Nanthawat Kosa,Poom Adisakwattana,Paron Dekumyoy 대한기생충학ㆍ열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.4
Tegumental and excretory-secretory proteins are reported as diagnostic antigens for human opisthorchiasis. Rhophilin associated tail protein1-like (OvROPN1L) protein of Opisthorchis viverrini sperm tail showed potential as a diagnostic antigen. The OvROPN1L recombinant fragments were assayed for diagnostic antigenicity for human opisthorchiasis using indirect ELISA. The strongest antigenic region was a N-terminus peptide of M1 - P56. One synthetic peptide (P1, L3-Q13) of this region showed the highest antigenicity to opisthorchiasis. Sera from other parasitic infections including Strongyloides stercoralis, hookworm, Taenia spp, minute intestinal flukes, Paragonimus spp showed lower reactivity to P1. Peptide P1 is located in the disordered N-terminus of ROPN1L supporting its suitability as linear epitope. In the Platyhelminthes the N-terminal sequence of ROPN1L is diverging with taxonomic distance further suggesting that peptide P1 has potential as diagnostic tool in the genus Opisthorchis/Clonorchis. It should be further evaluated in combination with peptides derived from other O. viverrini antigens to increase its diagnostic power.
Asymptomatic Human Paragonimiasis among the Karen People in Tak Province, Thailand: A Case Report
Teera Kusolsuk,Orawan Phuphisut,Wanna Maipanich,Somchit Pubampen,Surapol Sa-nguankiat,Akkarin Poodeepiyasawat,Nirundorn Homsuwan,Srisuchart Mongkolmoo,Tippayarat Yoonuan,Poom Adisakwattana,Udomsak Sil 대한기생충학열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.1