노쇠중인 밀 잎에서 Ascorbate - Glutathion 회로 관련 효소의 발달에 대한 Benzyladenine 의 효과

진창덕 (Chang Duck Jin) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.1

N/A

노쇠중인 밀잎에서 Benzyladenine 에 의한 막단백질의 안정화

진창덕 (Chang Duck Jin) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.2

N/A

무우 자엽의 노쇠에 미치는 Triacontanol 의 효과

진창덕(Chang Duck Jin),홍영남(Young Nam Hong) 한국식물학회 1989 Journal of Plant Biology Vol.32 No.4

Effects of triacontanol(TRIA) on several parameters of senescence including the changes of related enzyme activities were investigated in radish(Raphanus sativus L.) cotyledons developing in light. In senescing radish cotyledons, 1.0㎎ TRIA/l retarded the degradation of chlorophyll content. Moreover, it depressed the increases of malondialdehyde and H_2O_2 contents compared to the control. Catalase and superoxide dismutase activities were highly maintained but the increase of peroxidase activity was inhibited remarkably under the TRIA application. These results suggested that TRIA participated in the regulation of senescencce during the late part of cotyledon development where it delayed senescence through its action on free radical-associated enzymes and consequent metabolic turnover.

연화중 (緣化中) 유채자엽의 색소체 발달에 미치는 Benzyladenine 의 효과

진창덕(Chang Duck Jin),박영식(Young Sik Park),권영명(Young Myung Kwon),홍영남(Young Nam Hong) 한국식물학회 1986 Journal of Plant Biology Vol.29 No.4

Developmental changes of chlorophyll-protein (CP)-complex and plastid membrane proteins during the greening of rape (Brassica napus L.) cotyledons were examined in order to investigate the effect of benzyladenine (BA) on plastid development. The formation of CP-complexes was slightly promoted by BA treatment in early greening stage, at 24 h and 48 h after illumination. However, BA inhibited the development of CP-complexes at 72 h after illumination. On the profiles of plastid membrane proteins with greening time, it was found that the 24 kd protein was increased and the 56 kd protein was decreased in both water control and BA-treated cotyledons. However, the above two traits were retarded under BA treatment, respectively. From the obtained results, plastid development of rape cotyledon during greening was partially affected by interaction between light and BA dependent on its physiological age.

무우 유식물의 생장과 Peroxisome 효소 활성에 미치는 트리아콘타놀의 효과

진창덕(Chang Duck Jin),홍영남(Young Nam Hong) 한국식물학회 1984 Journal of Plant Biology Vol.27 No.4

The present study investigated the effects of triacontanol (TRIA) on plant growth and peroxisomal enzyme activities in radish seedlings. The optimum concentration of TRIA with respect to radish seedling bioassay was decided to 1.0 ㎎ 1^-1. In comparison to untreated controls (including Tween 20 treatment), 1.0 ㎎ 1^-1 TRIA treatment caused an increase in seed germination rate and root growth, but no stimulation in hypocotyl growth. Chlorophyll accumulation in cotyledon during early development stage increased rapidly, and degradation of chlorophyll in later stage due to the cotyledon senescence was noticeably retarded. Increase of soluble protein content in cotyledon at early period of development was observed. Isocitrate lyase and catalase activity was not significantly different in both the treated and the untreated plants. But, glycolate oxidase activity was inhibited by TRIA down to 20% against controls. Also, the increase of the activity of peroxidase, a leaf-senescence marker enzyme, was continuously retarded over control for 8 days of development. Based on above results, TRIA was found to be active in both the growth and the peroxisomal enzyme activities of radish seedlings.

애기장대(Arabidopsis thaliana)의 현탁배양세포괴로부터 식물체 재분화

김명덕(KIM Myoung Duck),김준철(KIM Joon Chul),진창덕(JIN Chang Duck),임창진(LIM Chang Jin),한태진(HAN Tae Jin) 강원대학교 기초과학연구소 1998 기초과학연구 Vol.9 No.-

Callus induction from leaf and stem explants of Arabidopsis thaliana was successfully obtained when leaf explants were cultured on MS medium containing 2.0 mg/L 2,4-D in the dark and also, when stem explants were cultured on CP medium containing 0.5 mg/L 2,4-D and 0.1 mg/L BAP. Explant-derived sliced calli were suspension-subcultured every week in CP liquid medium with 0.5 mg/L 2,4-D and 0.1 mg/L BAP in the dark, and shoot-forming cell clusters of nodular, pale yellow and knobby type were selected after 7-8 weeks of culture. Shoots were initiated from the green spots of the selected shoot forming calli cultured on MS regeneration medium containing 0.05 mg/L IAA, 7.0 mg/L 2-iP and 30 g/L sucrose under continous illumination for four weeks. Shoot regeneration frequency (calli regenerating at least one shoot) was more than 50%. For plant regeneration, excised shoots were transferred to hormone free medium for root initiation after 4 weeks of culture. The regenerants were bolting after 2 weeks of culture and formed in vitro flowering buds within bracts after 4 weeks of culture.

애기장대(Arabidopsis thaliana)의 엽육원형질체로부터 효율적인 식물체 재분화와 이들의 형태적 특성

김영덕(Kim Myoung Duck),김준철(Kim Joon Chul),진창덕(Jin Chang Duck),임창진(Lim Chang Jin),한태진(Han Tae Jin) 강원대학교 기초과학연구소 1999 기초과학연구 Vol.10 No.-

Protoplasts were isolated from the leaf mesophyll tissue of in vitro 4-weeks-old Arabidopsts thallana and cultured in MS liquid medium supplemented with 2.0 mg/L NAA, 0.5 mg/L BAP and 9% mannitol in the dark at 25°C. When protoplast-derived microcolonies were dehydrated, the frequency of callus induction enhanced approximately 7-fold higher compared with non-dehydrated microcolonies in CP medium. Fifty callus lines were selected from dehydrated microcolonies. Shoots were efficiently initiated from the green spots of the selected shoot forming calli cultured on MS regeneration medium supplemented with 0.05 mg/L IAA, 7.0 mg/L 2-iP and 30 g/L sucrose under continous illumination for 4 weeks. Shoot regeneration frequencies (calli regenerating at least one shoot) were 3.5%~56%. Histological observations of shoot forming callus revealed that tracheary elements initiated from inner compact cells, and that meristemoids developed to shoot primordia and shoots. Roots were induced from these regenerating shoots on MS medium without phytohomones. These regenerants were successfully transplanted into potting soil. Morphological characterization of 50 protoplast-derived plants showed that the frequency of normal type was 78%. Key words: Protoplast-derived microcolonies, shoot forming calli, green spots, regenerants

분리된 밀 엽록체의 단백질의 분해에 대한 Peptide Hydrolase 의 역할

진창덕,홍영남 ( Chang Duck Jin,Young Nam Hong ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.4

Intact chloroplasts were isolated mechanically from the primary leaves of 6-day old wheat seedlings (Triticum aestivum L.) and purified by using Ficoll gradient centrifugation. The proteolysis in isolated wheat chloroplasts during 24 h dark incubation at 30℃ was detected by following the degradation of the large subunit (LSU) of RuBPCase and the light harvesting chlorophyll protein (LHCP) complex by using SDS-polyacrylamide gel electrophoresis. In relation to the intrachloroplast localization of protease activity, the thylakoid fraction exhibited large activity while the stroma fraction did not. Time course of activities of acid endopeptidase and neutral aminopeptidase in isolated chloroplasts during 24 h dark incubation were as follows: the endopeptidase activity was high at the initial stage (0 to 2 h incubation ) and continuously lowered after 2 h incubation, on the other hand the aminopeptidase activity was very low at the start and gradually increased thereafter.

무우자엽의 엽록체 발달에 미치는 Triacontanol 의 효과

진창덕,홍영남 ( Chang Duck Jin,Young Nam Hong ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.1

The chloroplast development after application of triacontanol (TRIA) to germinating-radish seedling showed differential response on chlorophyll content, chlorophyll-protein(CP) complexes, thylakoid polypeptides, chloroplast and cytoplasmic ribosomal RNAs, and ribulose-1,5-bisphosphate carboxylase (RuBPCase) activity. In the chloroplast isolated from young (4-day-old) cotyledons, TRIA was efficient in promotion of all the above traits in contrast with non-treated control. Especially, total activity of RuBPCase, which is marker enzyme in photosynthesis, was increased by TRIA up to 11% over non-treated control. In senescing (7-day-old) cotyledon chloroplast, however, TRIA showed favorable influence on stabilization of structure and function of chloroplast with respect to these characters. The above results suggest the chloroplast as a useful assay system in a plant growth response to TRIA.

무 (Raphanus sativus L.) 자엽에서 산화질소 (Nitric oxide)에 의해 유도된 부정근 형성과정에 대한 칼슘의 효과

진창덕,Jin, Chang-Duck 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.3

분리된 무 자엽 조직에 산화질소 (nitric oxide: NO) 공여체인 sodium nitroprusside (SNP) 처리 시 농도 의존방식으로 부정근의 발달을 증진시켰다. 그러나 이러한 NO 증진 효과는 세포외 칼슘 chelator인 0.5 mM EGTA 또는 세포막 칼슘채널 차단제인 0.1 mM $LaCl_3$를 각각 $50\;{\mu}M$ SNP와 함께 혼합처리 시 반전되었다. 또한, 뿌리 발생에서 중심적 역할을 수행하는 것으로 알려진 guaiacol peroxidase (GPX)와 syringaldazine peroxidase (SPX)의 활성도가 SNP 단독 처리된 자엽에서 부정근이 형성되는 동안 현저히 증가하였다. 그러나, SNP와 $LaCl_3$ 혼합처리 시 SNP에 의해 유도된 GPX와 SPX 활성도 증가가 거의 증류수 대조구 수준으로 억제되었다. calmodulin의 anatagonist인 trifuoperazine 역시 SNP로 처리된 자엽에서 부정근 형성을 억제하여 발생된 뿌리의 개수와 길이를 감소시켰으며 동시에 GPX와 SPX를 불활성화 하였다. 결론적으로, 이들 결과는 칼슘이 GPX와 SPX 활성도 조절을 통해 부정근 유도를 이끄는 NO 반응에 포함되어 있음을 나타내는 것이다. The treatment of radish cotyledons with a nitric oxide (NO)-releasing substance, sodium nitroprusside (SNP) resulted in an increased adventitious root development in a dose-dependent manner. However, this NO-mediated enhancement effect was reversed when either 0.5 mM EGTA (an extracellular $Ca^{2+}$ chelator) or 0.1 mM $LaCl_3$ (a calcium channel blocker) was applied with $50\;{\mu}M$ SNP. Our results also showed that guaiacol peroxidase (GPX) and syringaldazine peroxidase (SPX) activities, which are known to play a key role in rooting, were more largely increased during adventitious root induction in the cotyledons treated with SNP. However, the treatment of cotyledons with SNP plus $LaCl_3$ inhibited the SNP-induced increases in the activities of both GPX and SPX. Trifluoperazine (TFP), an antagonist of calmodulin (a specific calcium-binding protein), also delayed adventitious root formation and significantly reduced the root length and number of the SNP-treated cotyledons as well as the deactivation of GPX and SPX enzymes. In conclusion, our results suggest that calcium is involved in the NO response leading to induction of adventitious root through a regulation of GPX and SPX.