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RISS 인기검색어
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- 저자 : ( Ming Chia Lai ) (검색결과 8 건)
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Chih-Jung Yao,Jyh-Ming Chow,Shuang-En Chuang,Chia-Lun Chang,Ming-De Yan,Hsin-Lun Lee,I-Chun Lai,Pei-Chun Lin,Gi-Ming Lai 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3
Background: KG-135, a standardized formulation enriched with Rk1, Rg3, and Rg5 ginsenosides, has been shown to inhibit various types of cancer cells; however, the underlying mechanisms are not fully understood. In this study, we explored its effects in A549 human lung cancer cells to investigate the induction of Forkhead Class box O3a (FOXO3a) and autophagy. Methods: Cell viability was determined by sulforhodamine B staining. Apoptosis and cell cycle distribution were analyzed using flow cytometry. The changes of protein levels were determined using Western blot analysis. Autophagy induction was monitored by the formation of acidic vesicular organelles stained with acridine orange. Results: KG-135 effectively arrested the cells in G1 phase with limited apoptosis. Accordingly, a decrease of cyclin-dependent kinase-4, cyclin-dependent kinase-6, cyclin D1, and phospho-retinoblastoma protein, and an increase of p27 and p18 proteins were observed. Intriguingly, KG-135 increased the tumor suppressor FOXO3a and induced the accumulation of autophagy hallmark LC3-II and acidic vesicular organelles without an increase of the upstream marker Beclin-1. Unconventionally, the autophagy adaptor protein p62 (sequestosome 1) was increased rather than decreased. Blockade of autophagy by hydroxychloroquine dramatically potentiated KG-135-induced FOXO3a and its downstream (FasL) ligand accompanied by the cleavage of caspase-8. Meanwhile, the decrease of Bcl-2 and survivin, as well as the cleavage of caspase-9, were also drastically enhanced, resulting in massive apoptosis. Conclusion: Besides arresting the cells in G1 phase, KG-135 increased FOXO3a and induced an unconventional autophagy in A549 cells. Both the KG-135-activated extrinsic FOXO3a/FasL/caspase-8 and intrinsic caspase-9 apoptotic pathways were potentiated by blockade of autophagy. Combination of KG- 135 and autophagy inhibitor may be a novel strategy as an integrative treatment for cancers.
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Yao, Chih-Jung,Chow, Jyh-Ming,Chuang, Shuang-En,Chang, Chia-Lun,Yan, Ming-De,Lee, Hsin-Lun,Lai, I-Chun,Lin, Pei-Chun,Lai, Gi-Ming The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3
Background: KG-135, a standardized formulation enriched with Rk1, Rg3, and Rg5 ginsenosides, has been shown to inhibit various types of cancer cells; however, the underlying mechanisms are not fully understood. In this study, we explored its effects in A549 human lung cancer cells to investigate the induction of Forkhead Class box O3a (FOXO3a) and autophagy. Methods: Cell viability was determined by sulforhodamine B staining. Apoptosis and cell cycle distribution were analyzed using flow cytometry. The changes of protein levels were determined using Western blot analysis. Autophagy induction was monitored by the formation of acidic vesicular organelles stained with acridine orange. Results: KG-135 effectively arrested the cells in G1 phase with limited apoptosis. Accordingly, a decrease of cyclin-dependent kinase-4, cyclin-dependent kinase-6, cyclin D1, and phospho-retinoblastoma protein, and an increase of p27 and p18 proteins were observed. Intriguingly, KG-135 increased the tumor suppressor FOXO3a and induced the accumulation of autophagy hallmark LC3-II and acidic vesicular organelles without an increase of the upstream marker Beclin-1. Unconventionally, the autophagy adaptor protein p62 (sequestosome 1) was increased rather than decreased. Blockade of autophagy by hydroxychloroquine dramatically potentiated KG-135-induced FOXO3a and its downstream (FasL) ligand accompanied by the cleavage of caspase-8. Meanwhile, the decrease of Bcl-2 and survivin, as well as the cleavage of caspase-9, were also drastically enhanced, resulting in massive apoptosis. Conclusion: Besides arresting the cells in G1 phase, KG-135 increased FOXO3a and induced an unconventional autophagy in A549 cells. Both the KG-135-activated extrinsic FOXO3a/FasL/caspase-8 and intrinsic caspase-9 apoptotic pathways were potentiated by blockade of autophagy. Combination of KG-135 and autophagy inhibitor may be a novel strategy as an integrative treatment for cancers.
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Delay of Surgery for Spinal Metastasis due to the COVID-19 Outbreak Affected Patient Outcomes
Chia-Jung Hsieh,Chun-Yu Wu,Yen-Heng Lin,Yu-Cheng Huang,Wen-Chi Yang,Tom Wei-Wu Chen,Wei-Li Ma,Wei-Hsin Lin,Feng-Ming Hsu,Furen Xiao,Shih-Hung Yang,Dar-Ming Lai,Chang-Mu Chen,Shin-Yi Chao,Fon-Yih Tsuan 대한척추신경외과학회 2023 Neurospine Vol.20 No.4
Objective: The present study is to analyze the effects of the coronavirus disease 2019 (COVID 2019) outbreak and the subsequent lockdown on the outcomes of spinal metastasis patients. Methods: The study was a retrospective analysis of data from a prospective cohort study. All patients underwent surgical intervention for spinal metastases between January 2019 and December 2021 and had at least 3 months of postoperative follow-up. The primary outcome was overall mortality during the 4 different stages (pre-COVID-19 era, COVID-19 pandemic except in Taiwan, national lockdown, lifting of the lockdown). The secondary outcomes were the oncological severity scores, medical/surgical accessibility, and patient functional outcome during the 4 periods as well as survival/mortality. Results: A total of 233 patients were included. The overall mortality rate was 41.20%. During the Taiwan lockdown, more patients received palliative surgery than other surgical methods, and no total en bloc spondylectomy was performed. The time from surgeon visit to operation was approximately doubled after the COVID-19 outbreak in Taiwan (75.97, 86.63, 168.79, and 166.91 hours in the 4 periods, respectively). The estimated survival probability was highest after the national lockdown was lifted and lowest during the lockdown. In the multivariate analysis, increased risk of mortality was observed with delay of surgery, with emergency surgery having a higher risk with delays above 33 hours, urgent surgery (below 59 and above 111 hours), and elective surgery (above 332 hours). Conclusion: The COVID-19 pandemic and related policies have altered daily clinical practice and negatively impacted the survival of patients with spinal metastases.
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Spray Characteristics on the Electrostatic Rotating Bell Applicator
Kyoung-Su Im,Ming-Chia Lai,Suck-Ju Yoon 대한기계학회 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.12
The current trend in automotive finishing industry is to use more electrostatic rotating bell (ESRB) need space to their higher transfer efficiency. The flow physics related with the transfer efficiency is strongly influenced by operating parameters. In order to improve their high transfer efficiency without compromising the coating quality, a better understanding is necessary to the ESRB application of metallic basecoat painting for the automobile exterior. This paper presents the results from experimental investigation of the ESRB spray to apply water-borne painting. The visualization, the droplet size, and velocity measurements of the spray flow were conducted under the operating conditions such as liquid flow rate, shaping airflow rate, bell rotational speed, and electrostatic voltage setting. The optical techniques used in here were a microscopic and light sheet visualization by a copper vapor laser, and a phase Doppler particle analyzer (POPA) system. Water was used as paint surrogate for simplicity. The results show that the bell rotating speed is the most important influencing parameter for atomization processes. Liquid flow rate and shaping airflow rate significantly influence the spray structure. Based on the microscopic visualization, the atomization process occurs in ligament breakup mode. which is one of three atomization modes in rotating atomizer. In the spray transport zone, droplets tend to distribute according to size with the larger drops on the outer periphery of spray. In addition, the results of present study provide detailed information on the paint spray structure and transfer processes.
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Spray Characteristics on the Electrostatic Rotating Bell Applicator
Im, Kyoung-Su,Lai, Ming-Chia,Yoon, Suck-Ju The Korean Society of Mechanical Engineers 2003 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.17 No.12
The current trend in automotive finishing industry is to use more electrostatic rotating bell (ESRB) need space to their higher transfer efficiency. The flow physics related with the transfer efficiency is strongly influenced by operating parameters. In order to improve their high transfer efficiency without compromising the coating quality, a better understanding is necessary to the ESRB application of metallic basecoat painting for the automobile exterior. This paper presents the results from experimental investigation of the ESRB spray to apply water-borne painting. The visualization, the droplet size, and velocity measurements of the spray flow were conducted under the operating conditions such as liquid flow rate, shaping airflow rate, bell rotational speed, and electrostatic voltage setting. The optical techniques used in here were a microscopic and light sheet visualization by a copper vapor laser, and a phase Doppler particle analyzer (PDPA) system. Water was used as paint surrogate for simplicity. The results show that the bell rotating speed is the most important influencing parameter for atomization processes. Liquid flow rate and shaping airflow rate significantly influence the spray structure. Based on the microscopic visualization, the atomization process occurs in ligament breakup mode, which is one of three atomization modes in rotating atomizer. In the spray transport zone, droplets tend to distribute according to size with the larger drops on the outer periphery of spray. In addition, the results of present study provide detailed information on the paint spray structure and transfer processes.
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High-Pressure Diesel Injection Studied by Time-Resolved X-Ray Phase-Contrast Imaging
( Kamel Fezzaa ),( Wah Keat Lee ),( Seong Kyun Cheong ),( Christopher F. Powell ),( Ming Chia Lai ),( Jin Wang ) 한국액체미립화학회 2005 한국액체미립화학회 학술강연회 논문집 Vol.2005 No.-
With high-energy and high-brilliance x-ray beams available at third-generation synchrotron sources, propagation-based phase-enhanced imaging was developed as a unique metrology technique to visualize the internal structure of high-pressure fuel-injection nozzles. We have visualized the micrometer-scale machining and finishing defects inside 200-μm fuel-injection nozzles in a 3-mm-thick steel housing using phase-enhanced x-ray imaging. Because of the phase enhancement, for the first time, this new microimaging-based metrology technique has been used to directly study the highly transient pintle motion in the nozzles in situ and in real time with 10-μs temporal resolution, which is virtually impossible by any other means. The needle motion has been shown to have the most direct effect on the fuel-jet structure and spray formation immediately outside of the nozzle. In addition, a transient computational fluid dynamics simulation of in-nozzle fuel flow is in progress to correlate the fuel jets immediately outside the orifice and the in-nozzle flow.
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