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해양환경 기반 한국 연근해 어장 민감도 평가 기술 개발
주희태 ( Huitae Joo ),유만호 ( Manho Yoo ),윤상철 ( Sang Chol Yun ),김창신 ( Chang Sin Kim ),이민욱 ( Min Uk Lee ),김상일 ( Sangil Kim ),박경우 ( Kyoung Woo Park ),황재동 ( Jae-dong Hwang ),오현주 ( Hyun Joo Oh ),윤석현 ( Seok-hyu 한국수산과학회 2021 한국수산과학회지 Vol.54 No.4
Although scientist have been reporting recently that changes in ocean environment influence the species composition, movements, and growth of fish in Korea waters. Previous studies on fish vulnerability owing to climate changes are insufficient to explain the effect of fluctuating ocean environments on fisheries ground. In this study, we suggested a method for the assessment of fisheries sensitivity to various factors in ocean environments in Korean waters. To evaluate the fisheries sensitivity, catch data (Chub mackerel, Hairtail, Common squid, small yellow croaker) from National federation of fisheries cooperatives in Korea (1991-2017) and oceanographic data from Korea Ocean Data Center (KODC; 1960-2017) were normalized using the z-score method. Thereafter, the fisheries sensitivity was calculated using the difference between the catch data and the oceanographic data. Finally, the fisheries sensitivity was evaluated based on evaluation grade ratings. Result revealed that in the south sea, variability in catch data was obviously higher than environmental fluctuation (evaluation grade 1), indicating that catch variability in response to environmental change is most sensitive in the south sea among Korean waters in 2017. These results would be helpful for fishery management and policy for sustainable yield in Korean waters.
Determination of protein phosphorylation by polyacrylamide gel electrophoresis
Chang-Ro Lee,Young-Ha Park,Huitae Min,Yeon-Ran Kim,Yeong-Jae Seok 한국미생물학회 2019 The journal of microbiology Vol.57 No.2
Phosphorylation is the most important modification for protein regulation; it controls many signal transduction pathways in all organisms. While several tools to detect phosphorylated proteins have been developed to study a variety of basic cellular processes involving protein phosphorylation, these methods have several limitations. Many proteins exhibit a phosphorylation-dependent electrophoretic mobility shift (PDEMS) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the molecular mechanism responsible for this phenomenon has been elucidated recently. The method for detecting phosphorylated proteins can be simplified by the application of the PDEMS. Herein, we present a novel simple method to detect protein phosphorylation, which is based on the construction of a variant protein displaying a PDEMS. The PDEMS of proteins is caused by the distribution of negatively charged amino acids around the phosphorylation site, i.e. an electrophoretic mobility shift (EMS)-related motif (ΘX1-3ΘX1-3Θ, where Θ corresponds to an acidic or phosphorylated amino acid and X represents any amino acid). The EMS-related motif can be constructed by the introduction of a negative charge by phosphorylation; it results in the decreased binding of SDS to the proteins, consequently inducing the retardation of the mobility of the protein during SDS-PAGE. Based on these molecular analyses of the PDEMS, a protein with the EMSrelated motif is designed and used to determine the in vivo phosphorylation state of the protein. This method may be used as a general strategy to easily measure the ratio of protein phosphorylation in cells.
Anti-Melanogenic Effect of Cannabis sativa Stem Extracts Fermented with Weissella paramesenteroides
Kim Taehyun,Kim Jin-Woo,Min Huitae,Park Jisu,Kim Taejung,Kim Geun-Hyeong,Park Byung-Joon,Kim Jeong Kook,Park Young-Tae,Kim Jin-Chul,Ham Jungyeob 한국미생물·생명공학회 2023 한국미생물·생명공학회지 Vol.51 No.3
Cannabis sativa (CS) has been in the spotlight not only for its medical uses but also as a raw material for cosmetics. As fermented cosmetics are known to have various health benefits, they have been extensively researched. Here, we investigated the characteristics of CS stems fermented using various gut microbes. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay and melanin content analysis revealed that melan-a cells containing CS stems fermented with Weissella paramesenteroides (CSWP) showed considerably reduced melanin content. Additionally, CSWP downregulated the expression of several melanogenesis factors, tyrosinase-related protein-1, and tyrosinase-related protein-2. This study suggests that the anti-melanogenic effect of CSWP could provide a new basis for the development of skinlightening agents.
Choi, Soo In,Kim, Nayoung,Lee, Sun Min,Nam, Ryoung Hee,Kang, So Ra,Song, Chin-Hee,Park, Young-Tae,Min, Huitae,Kim, Yeon-Ran,Seok, Yeong-Jae Korean Society of Cancer Prevention 2019 Journal of cancer prevention Vol.24 No.1
<P><B>Background</B></P><P>High-fat diet is known to be implicated in the pathogenesis of various metabolic disorders related to an inflammatory response. The aim of this study was to investigate the influence of high-fat diet for intestinal acetic acid and butyric acid concentrations which are related to inflammation-associated colon cancer risk.</P><P><B>Methods</B></P><P>Both male and female rats of 6, 31, 74 and 104-week of age were fed chow diet or high-fat diet for 8 weeks. Body weight and food intake were measured weekly during the feeding period. Intestinal acetic acid and butyric acid levels were measured by high-performance liquid chromatography from luminal contents of ileum and cecum.</P><P><B>Results</B></P><P>Male rats showed greater weight change than female rats in every age. Calorie-adjusted food intake was also higher in male rats compared to female rats. Male rats showed similar intake of food in every age while 31-week old female rats showed increased intake, which was decreased at 74-week and 104-week of age. The ileal acetic acid concentration was increased in male rats fed high-fat diet, while female rats fed high-fat diet showed no significant change in the ileal acetic acid level. On the other hand, butyric acid almost disappeared in high-fat diet fed rats regardless of sex.</P><P><B>Conclusions</B></P><P>High-fat diet increases the intestinal acetic acid concentration while reducing the butyric acid concentration which may account for increased risk of inflammation-associated colon cancer.</P>
Changes in Cecal Microbiota and Short-chain Fatty Acid During Lifespan of the Rat
( Soo In Choi ),( Joo Hee Son ),( Nayoung Kim ),( Yong Sung Kim ),( Ryoung Hee Nam ),( Ji Hyun Park ),( Chin-hee Song ),( Jeong Eun Yu ),( Dong Ho Lee ),( Kichul Yoon ),( Huitae Min ),( Yeon-ran Kim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2021 Journal of Neurogastroenterology and Motility (JNM Vol.27 No.1
Background/Aims The gut microbiota regulates intestinal immune homeostasis through host-microbiota interactions. Multiple factors affect the gut microbiota, including age, sex, diet, and use of drugs. In addition, information on gut microbiota differs depending on the samples. The aim of this study is to investigate whether changes in cecal microbiota depend on aging. Methods Gut microbiota in cecal contents of 6-, 31-, and 74-week-old and 2-year-old male Fischer-344 rats (corresponding to 5-, 30-, 60-, and 80-year-old humans in terms of age) were analyzed using 16S ribosomal RNA metagenome sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) based on the Kyoto Encyclopedia of Genes and Genomes orthology. Moreover, short-chain fatty acid (SCFA) level in cecum and inflammation related factors were measured using real-time quantitative polymerase chain reaction and enzyme linked immunosorbent assay. Results Alpha and beta diversity did not change significantly with age. At the family level, Lachnospiraceae and Ruminococcaceae, which produce SCFAs, showed significant change in 31-week-old rats: Lachnospiraceae significantly increased at 31 weeks of age, compared to other age groups, while Ruminococcaceae decreased. Butyrate levels in cecum were significantly increased in 31-week-old rats, and the expression of inflammation related genes was increased followed aging. Especially, EU622775_s and EU622773_s, which were highly abundance species in 31-week-old rats, showed significant relationship with butyrate concentration. Enzymes required for producing butyrate―acetyl-CoA transferase, butyryl-CoA dehydrogenase, and butyrate kinase―were not predicted by PICRUSt. Conclusions Major bacterial taxa in the cecal lumen, such as Lachnospiraceae, well-known SCFAs-producing family, changed in 31-week-old rats. Moreover, unknown species EU622775_s and EU622773_s showed strong association with cecal butyrate level at 31 weeks of age. (J Neurogastroenterol Motil 2021;27:134-146)