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- 저자 : 황창일 ( Hwang Chang Il ) (검색결과 3 건)
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황창일,현성호,이철태 ( Chang Il Hwang,Seong Ho Hyun,Chul Tae Lee ) 한국공업화학회 1992 공업화학 Vol.3 No.3
함회토류광물로부터 3가지 회토류성분의 추출공정이 국산 monazite에 대한 적절한 추출공정을 찾기위해 조사되었다. 본 연구에서 조사된 3가지 공정은 황산법, 알칼리법및 황산암모늄 분해법이었다. 실험결과로부터 황산법 및 알칼리법이 국산 monazite에 더욱 효과적인 추출공정이었음이 조사되었다. 국산 monazite에 대한 황산법의 적절한 반응조건은 반응온도 210℃, 반응시간 40분, monazite에 대한 H_2SO_4의 고액비 1.5, 황산의 농도 95%였으며, 이 조건하에서 98%의 회토류성분이 추출되었다. 또한 알칼리법에 대한 적절한 반응조건은 반응온도 140℃, monazite에 대한 NaOH의 무게비 3.0, NaOH의 농도 50%, 침출시간 3시간이었으며, 이 조건하에서 97%의 회토류성분이 추출되었다. Three type extraction processes of rare earth metal component from rare earth metal bearing ore were tested to find an appropriate extraction method for domestic monazite ore. Three processes tested in this study were sulfuric acid digestion, caustic soda leaching and decomposition with (NH_4)_2SO_4. From the overall results, both caustic soda leaching and sulfuric acid digestion were better extraction processes for domestic monazite ore. The proper conditions of sulfuric acid digestion for domestic monazite ore were reaction temperature 210℃, reaction time 40 min, weight ratio of H_2SO_4 to monazite ore 1.5 and concentration of H_2SO_4 95%. Under these conditions, 98% of rare earth metal component was extracted and also the reasonable conditions for caustic soda leaching were reaction temperature 140℃, weight ratio of NaOH to monazite 3.0, concentration of caustic soda solution 50% and leaching time 3hrs. Under these conditions, 97% of rare earth metal component was extracted.
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MCF-7 세포주의 γ선에 의한 DNA 손상 반응 유전자 발현 양상의 분석
박지윤 ( Ji Yoon Park ),황창일 ( Chang Il Hwang ),박웅양 ( Woong Yang Park ),김진규 ( Jin Kyu Kim ),채영규 ( Young Gyu Chai ) 한국환경생물학회 2005 환경생물 : 환경생물학회지 Vol.23 No.1
N/A Cell response to genotoxic agents is complex and involves the participation of different classes of genes including cell cycle control, DNA repair and apoptosis. In this report, we presented a approach to characterize the cellular functions associated with the altered transcript profiles of MCF-7 exposed to low-dose in vitro gamma-irradiation. We used the method of human 2.4 k cDNA microarrays containing apoptosis, cell cycle, chromatin, repair, stress and chromosome genes to analyze the differential gene expression characterization that were displayed by radiation-exposed cell, human breast carcinoma MCF-7 cell line, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. Among these genes, 66 were up-regulated and 49 were down-regulated. Specific genes were concomitantly induced in the results. Cyclin dependent kinase 4 (Cdk4) is induced for starting the cell cycle. This regulation is required for a DNA damage-induced G1 arrest. In addition to, an apoptotic pathways gene Bcl- w was concomitantly induced. Mismatch repair protein homologue-1 (hMLH1), a necessary component of DNA mismatch protein repair (MMR), in G2-M cell cycle checkpoint arrest. The present study provides new information on the molecular mechanism underlying the cell response to genotoxic stress, with relevance to basic and clinical research.
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MCF-7 세포주에서 γ선에 의한 세포신호 전달 관련 유전자의 발현 양상의 분석
박지윤 ( Park Ji Yun ),황창일 ( Hwang Chang Il ),박웅양 ( Park Ung Yang ),김진규 ( Kim Jin Gyu ),채영규 ( Chae Yeong Gyu ) 한국환경생물학회 2003 환경생물 : 환경생물학회지 Vol.21 No.1
N/A There is considerable evidence that ionizing radiation (IR) mediates checkpoint control, repair and cell death. In this study, we have used a high density microarray hybridization approach to characterize the transcriptional response of human breast carcinoma MCF-7 cell line to y-radiation, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. We found that exposure to γ-ray alters by at least a logs factor of 1.0 the expression of 115 known genes. Of the 66 genes affected by ^-radiation, 49 are down-regulated. In our results, the cellular response to irradiation includes induction of the c-jun and EGRI early response genes. The present work has examined potential cytoplasmic signaling cascades that transduce IR-induced signals to the nucleus. 40S ribosomal protein s6 kinase modulates the activities of the mitogen activated protein kinase (MAPK) and c-Jun NH₂-terminal kinase (JNK1) cascades in human monocytic leukemia (U937/pREP4) cells. 14-3-3 family members are dimeric phosphoserine-binding proteins that participate in signal transduction and checkpoint control pathways.
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