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황원무 ( Won Moo Hwang ),이성모 ( Sung Mo Lee ),황현순 ( Hyun Soon Hwang ),한정희 ( Jeong Hee Han ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.2
This experiment was executed to control effectively endemic disease of swine farms in Incheon metropolitan city. Mainly using PigMon program which had been developed by the College of Veterinary Medicine in the University of Minnesota(USA), we examined lesions of gastric ulcer and interstitial nephritis additionally. 446 heads of pigs shipped from 5 farms in Incheon to a slaughterhouse from June in 2003 to May in 2004 were examined. Infection rates by farms were obtained as follows; 1. Pneumonia was varied from 34.6% to 74.1% and pneumonic score was 1.47~7.06. As for atrophic rhinitis, four farms were 100% and one farm was 89.5% and rhinitis score was 1.3~3.2. 2. The infection rate of pericarditis and peritonitis was 1.0~3.9% and liver white spots, papular dermatitis were observed in pigs of 9.8~29.7%, 16.7~51.4% respectively. 3. The outbreak rates of interstitial nephritis, lesions of ileal thickening and gastric ulcer were 15.4~24.1%, 7.7~13.5%, and 62.7%~86.2% respectively.
원유중(原乳中) 미량(微量) 설파제(劑) 검출방법(檢出方法) 비교(比較)에 대한 실험적(實驗的) 연구(硏究)
황원무 ( Won Moo Hwang ),이성모 ( Song Mo Lee ),손봉환 ( Bong Whan Sohn ),이원창 ( Won Chang Lee ) 한국가축위생학회 1997 韓國家畜衛生學會誌 Vol.20 No.1
The sulfonamide is one of potentiative antimicrobial agents which is being used widely in veterinary medicine for control of several animal diseases such as mastitis as well as for promotion of growth. However, the misusages of sulfonamides in food producing animals, especially cattle produce several considerable problems in human health caused from residues of this antibiotic in milk product. To determine the most effective analytical methods for residual sulfonamides in raw milk, this study was performed comparatively using by some applicable screening detecting method such as TTC, Charm II test (sulfonamides), and Lactek tests (sulfamethazine kit). The positive result from screening tests was confirmed by HPLC method. Milk samples (540 raw milks) were collected from dairy farms. Results of this study are summariezed as follows 1. All samples (540 raw milks) showed negative response from TTC test, however, 18 raw milks of those samples responded positively to Charm II test. 2. By Lactek test, residual sulfamethazine was detected from 4 raw milks. Fifteen raw milks of 18 samples which were classified as positive one by Charm II test, showed positive response. 3. Retention time of sulfonamides added at the level of 100ppb into skim milk was ranged from 1.55 minute to 23.3 minute. Recovery rates of sulfonamides were variable from 6.7% upto 94.2% depended on the types of sulfonamide. 4. Single type of sulfonamides was detected from 10 raw milk samples, 2 types of sulfonamides from 3 samples and 3 types from 2 raw milks by HPLC. 5. Sulfonamides was detected in this study were 5 types :11 samples for sulfisomidine, 5 samples for sulfamethazine, 3 samples for sulfadimethoxine, 2 samples for sulfathiazole and 1 sample for sulfadiazine. 6. The highest levels of residual sulfonamides Was 210.3 ppb of sulfamethazine but the lowest concentration of residue was 2.2 ppb of sulfamethazine and sulfisornidine, respectively. Number of samples detected positively in this experiment were belows : above 100 ppb for 1 sample (4.5%) (sulfamethazine), 50 - 100 ppb for 4 samples (18.1 %) (each 2 samples for sulfamethazine and sulfisomidine, respectively), 25-50ppb for 6 samples (27.1%) (2 sulfisomidine, each 1 sample for sulfadiazine, sulfadimethoxine, sulfamethazine and sulfathiazole, respectively), 10-25ppb for 3 samples (13.7%) (3 sulfisomidine), and below loppb for 8 samples (36.4%) (4 sulfisomidine, 2 sulfadimethoxine and each 1 for sulfamethazine and sulfathiazole).
인천지역 도축장에서 생산된 돼지고기의 미생물 오염도 조사
황원무 ( Won Moo Hwang ),이성모 ( Sung Mo Lee ),황현순 ( Hyun Soon Hwang ),한정희 ( Jeong Hee Han ) 한국가축위생학회 2004 韓國家畜衛生學會誌 Vol.27 No.1
For measuring general bacterial count and Escherichia coli count, the standard plate count was used to conduct a test on 113 cases of pork carcass surfaces from slaughterhouses in Incheon area from January to February 2003. And Salmonella spp, Staphylococcus aureus and Listeria monocytogenes was used to conduct a test on 68 cases of pork carcass surfaces and 76 cases of feces, that is to say, a total of 144 cases. The results were obtained as follows: In the case of general bacterial count, 29 cases(25.7%) were in the range of 100~999 and 62 cases(54.9%) were in the range of 1,000~9,999 and 22 cases(19.4%) were in the range of 10,000~99,999. Meanwhile as regards E coli count, 22 cases(19.4%) were in the range of 1~9 and 69 cases(61.2%) were in the range of 10~99 and 22 cases(19.4%) were in the range of 100~999. On 68 cases of pork carcass surfaces, 7 strains(10.2%) of Salmonella spp, and 12 strains(17.6%) of S aureus were detected and 27 strains(39.7%) of L monocytogenes, respectively. As for the detected Salmonella spp, 6 strains of the B group, 3 strains of S enterica subsp salame and 2 strains of S typhimuriwn were detected, respectively. On 76 cases of feces, 14 strains(18.4%) of Salmonella spp, and 15 strains(19.7%) of L monocytogenes and 14 strains(18.4%) of S aureus were detected respectively. As for the detected Salmonella spp, 6 strains of the B group, 4 strains of S derby and 8 strains of the C group, 5 strains of S rissen were detected, respectively. All of 42 strains of L monocytogenes were type 1. As a result of conducting a toxin test on the detected S aureus, all of 26 strains were found to be non-toxin.
황원무 ( Won Moo Hwang ),김명희 ( Myeong Hee Kim ),윤가리 ( Ga Ri Yun ),이길봉 ( Gil Bong Lee ),고종명 ( Jone Myeung Go ),김용희 ( Yong Hee Kim ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2
This study was conducted in order to get basic data on standards and specifications of health and functional foods. A total of 101 kinds of functional healths foods were examined during the period of January to December 2005. Among them, 89 kinds were local products and 12 kinds were imported products. Test items were 6 kinds of heavy metals(lead, cadmium, mercury, copper, zinc and manganese), 5 bacteria(common bacteria, coliform group, E coli, Bacillus cereus and Clostridium perfringens), and 6 preservatives(sorbic acid, benzoic acid, DHA, ethyl paraben, propyl paraben and butyl paraben). As a result of heavy metals, cadmium, was detected from the glucosamine products by 1.52ppm in average, mercury from cereal products by 0.004ppm in average, and lead from chlorella foods by 3.48ppm in average. Bacteria were identified from 3 cereal products, and amount of common bacteria were about 4.8×105cfu/g in average. E Coli and Coliform group were isolated from 2 products and 4 products, respectively. All of those products were flour meal products. Any of 6 kinds of preservatives was not detected from all the products. It is thought that these test results will be available as basic data for enactment of relevant laws and regulations for production and control of safer and more hygienic foods in the future because the standards of the harmful heavy metals are not complete or available yet according to the Food Sanitation Law and the Functional Healths Foods Law.