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최남훈,김경욱 단국대학교 치의학연구소 1994 논문집 Vol.6 No.2
Bone graft in the field of oral and maxillofacial srugery is being used in congenital deformities, trauma, infection, & acquired deformities followed by tumor resection, and plastic augmentation of lowered alveolar ridge height. Demineralized bone is mainly composed of organic substance since it is prepared by removing inorganic component of apatite in bone and can be used in various configurations. The mechanism of bone formation after grafting demineralized bone is characterized by cartilage formation followed by subsequent bone formation as in endochondral bone formation induced by osteogenic protein within matrix of demineralized bone. Rejection occurs against untreated allogeneic bone of which the most cellular components are known to possess graft antigen. The major provoking factor of this immune reaction was confirmed to be subcellular particle similar to DNA in bone. In order to avoid stimulation of host immune reaction and reduce immunity of graft material, a variety of methods are recommended such as heating, irradiation, simple freezing and freeze-drying so that allogeneic bond graft doesn't stimulate immune response of host tissue. The purpose of this study was to compare simple demineralized bone powder with freeze-dried demineralized bone powder in the capacity of healing and bone induction. Demineralized bone powders in the range of 500 - 1000㎛ particle size were grafted into femur of rabbit and the healing processes were observed histologically through light and electron microscope. The results were as follows ; 1. Fibrous capsule with irregular resorption pattern around grafted bone was observed after 1 week in both group. 2. After 2 weeks, the degree of inflammatory cell infiltration in the experimental group was observed to be less than that of control group while the amount of new bone formation and osteoblastic apposition were relatively more active than control group. 3. Somewhat large cells of chondrocyte-like appearance were observed in both experimental and control group from the 2nd week of experiment. 4. While the overall histological feature of the 3-week experimental group showed similar patten to that of 4-week control group, activity of grafted bone matrix was great and the appearance of osteoclast was sparsely observed. 5. In EM feature of the 4th week, processes from cell body of osteocytes in lacunae were found to be extended between canaliculi. 6. From the above mentioned results, it might be concluded that freeze-dried demineralized bone is better in the aspects of new bone formation than simple dried demineralized bone.
최남훈,김경욱 단국대학교 치의학연구소 1990 논문집 Vol.2 No.-
The purpose of this experiment was to study the histologic changes of submandibular glands of which ducts were cut and ligated. In A-group of 4 dogs, the right submandibular ducts were intact as control, and ducts were ligated and the left submandibular ducts were cut. The submandibular glands were studied histologically after 2, 5, 8, and 12 weeks. The results were as follows: 1. The submandibular glands of which ducts were found as normal as control submandibular glands. 2. The submandibular glands of which ducts were ligated were atrophied in contrast with the submandibular glands of which ducts were cut. 3. The cut submandibular ducts did not cause histologically atrophic changes on submandibular glands until 12 weeks.
金慶勖,崔南勳 大韓顎顔面成形再建外科學會 1990 Maxillofacial Plastic Reconstructive Surgery Vol.12 No.2
The purpose of this experiment was to study the histologic changes of submabdibular glands of which ducts were cut and ligated. In A-Group of 4 dogs, the right submandibular ducts were intact as control, and ducts were ligated and the left submandibular ducts were cut. The submandibular glands were studied histologically after 2,5,8, and 12 weeks. The results were as follows : 1. The submandibular glands of which ducts were found as normal as control submandibular glands. 2. The submandibular glands of which ducts were ligated were atrophied. 3. The cut submandibular ducts did not cause histologically atrophic changes on submandibular glands until 12 weeks.