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      • 고온환경에 있어서 면양의 제일위내 VFA 조성비율이 일반임상소견 및 Insulin 분필반응에 미치는 영향 - VFA-TG 인공영양사육법의 응용 -

        홍경선,정태영,좌야굉명,지하농랑,중와방야,안보계일 한국축산시설환경학회 1997 축산시설환경학회지 Vol.3 No.1

        This study was conducted to investigate the combined effects of VFA composition of rumen fluid and heat exposure (30${\pm}$2$^{\circ}C$) on the general clinical view and insulin secretory response to glucose in sheep. The total infusion of nutrients was examined in sheep via the technique of continuous alimentation. Four adult Suffolk sheep fitted with a permanent ruminal cannula and a simple T-shaped duodenal cannula were used. A peristaltic pump was used to infuse the solutions of volatile fatty acid triglycerides (VFA-TG) consisting of 70 triacetin : 20 tripropionin : 10 tributyrin (low propionin division: LP) and 50 triacetin : 40 tripropionin : 10 tributyrin (high propionin division: HP) on the basis of energy and minerals into the rumen, and casein solution into the duodenum. The effects of heat exposure and type of the levels of VFA-TG solutions on the insulin secretory response to glucose in sheep were investigated by using hyperglycemic clamp (HGC) technique. The results obtained are summarized as follows: 1. During the heat exposure (latter half of the infusion period), respiration rate, heart rate and rectal temperature increased (P<0.01, P<0.01, P<0.05), but the levels of VFA-TG solutions (LP and HP division) did not affect the general clinical view except for the heart rate. 2. In the HGC technique, glucose infusion rate (GIR) and mean plasma insulin increments (MPII) tended to be ower in the heat exposure than in the thermoneutral environment, but no significant difference was found among the treatments. GIR and MPII remained unchanged between the levels of VFA-TG solutions. 3. In the HGC technique, ratio of MPII to GIR (MPII/GIR) which represents pancreatic ${\beta}$-cell response to glucose stimulation remained unchanged among the treatments.

      • KCI우수등재

        저온환경에 있어서 VFA - TG 인공영양사육 면양의 제1위내 VFA 조성비율이 일반임상소견 및 제1위액성상에 미치는 영향

        홍경선,정태영,좌야굉명,중도방야,지하농랑,안보가일 한국축산학회 1997 한국축산학회지 Vol.39 No.5

        This study was conducted to investigate the combined effects of VFA composition of rumen fluid and cold exposure on the general clinical view and ruminal fermentation characteristics in sheep. Each infusion group was subjected to either thermoneutral (20±2 ℃) or cold environment (2±2℃) for 4 days. The total infusate of nutrients was examined in sheep via the technique of continuous alimentation. Four adult Suffolk sheep fitted with a permanent ruminal cannula and a simple T-shaped duodenal cannula were used. A peristaltic pump was used to infuse the solutions of volatile fatty acid triglycerides (VFA-TG, triacetin: tripropionin: tributyrin) consisting of 70:20:10 (low propionin division: LP) and 50:40:10 (high propionin division: HP) on the basis of energy and minerals into the rumen, and casein solution into the duodenum. The results obtained are summarized as follows: 1. During the cold exposure (latter half of the infusion period), respiration rate decreased (P$lt;0.10), but heart rate tended to increase in the thermoneutral environment. And respiration rate had no significant difference between the levels of VFA-TG solutions (LP and HP division), but heart rate was higher in the HP division than in the LP division (P$lt;0.10). 2. In the HP division, rectal temperature was lower (P$lt;0.001) in the cold exposure than in the thermoneutral environment, but remained unchanged by the temperature treatment in the LP division. During the thermoneutral environment, rectal temperature was higher in the HP division than in the LP division (P$lt;0.01), but remained unchanged by the levels of VFA-TG solutions in the cold exposure. 3. Urine pH values maintained the value of the thermoneutral environment during the latter half of the infusion period, and was lower (P$lt;0.05) in the HP division than in the LP division. 4. Ruminal total volatile fatty acid (VFA) concentration tended to increase in the cold exposure compared to that in the thermoneutral environment, and did not affect with the levels of VFA-TG solutions. 5. In the LP division, molar percent of acetate was higher (P$lt;0.10), but molar percent of propionate was lower (P$lt;0.05) in the cold exposure than in the thermoneutral environment. As a result, the ratio of acetate to propionate (A:P ratio) increased more (P$lt;0.10) in the cold exposure than in the thermoneutral environment. In the HP division, molar percents of acetate, propionate and butyrate were not affected by the temperature treatments. The results showed that the A:P ratio was not affected by the environmental temperature treatments. However the ratio was increased in the LP division than in the HP division (P$lt;0.001). 6. Ruminal pH values was not affected by the temperature treatments, but was higher (P$lt;0.001) in the LP division than in the HP division. And ruminal pH values tended to be higher in the HP division than in the LP division. 7. Infusion of VFA-TG into the rumen caused a marked increase (P$lt;0.01) in ruminal TG concentration during the cold exposure (latter half of the infusion period), but it was not affected by the levels of VFA-TG solutions.

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