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RISS 인기검색어
- 검색키워드
- 저자 : 정은빈 ( Eun Bin Jung ) (검색결과 3 건)
- 무료
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참붕어, Pseudorasbora parva의 인두흡충, Clinostomum complanatum 피낭유충 감염증
정은빈 ( Eun Bin Jung ),김동완 ( Dong Wan Kim ),김진도 ( Jin Do Kim ),박성우 ( Sung Woo Park ) 한국어병학회 2012 한국어병학회지 Vol.25 No.2
Many metacercariae in the musculature of southern top mouthed minnow (Pseudorasbora parva) cultured at a farm in Chonbuk Province. The excysted metacercariae looked yellowish white and were 4.02±1.80 ㎜(1.66-5.81) in length and 1.27±0.27 ㎜(1.01-1.64) in width for acetocarmin-stained specimens. The oral sucker measuring 0.18±0.05 ㎜ (0.11-0.24) in length and 0.23±0.07 ㎜(0.15-0.33) in width, was located at the anterior end of the body. The ventral sucker situated at the fourth third of the body were measured 0.47±0.08 ㎜(0.35-0.53) in length and 0.55±0.09 ㎜ (0.43-0.63) in width. The tongue-shaped metacercariae were identified as Clinostomum complanatum on the basis of morphological characteristics. The metacercaria was also confirmed to be C. complanatum using PCR reaction with C. complanatum-specific primer sets, Trem 18S F and Ccom670 R. No histopathological changes in the infected fish were observed except necrosis and exfoliation of epidermis in the skin and atrophy of muscle fibers.
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양식 동남아산 뱀장어, Anguilla bicolor의 Anguillid herpesvirus-1 (AngHV-1) 감염증
박성우 ( Sung Woo Park ),정은빈 ( Eun Bin Jung ),김동완 ( Dong Wan Kim ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
Diseased eel (Anguilla bicolor) displayed severe hemorrhages in the gills, and congestion and swelling in the liver. During the epizootic, the water temperature was 28℃ and the morality rates were about 5%. No parasites were found on the gills and skin. Bacteria were not cultured from any internal organs using TSA or SS agar at 28℃ for 48 hrs. Histopathologically, the gills showed epithelial hyperplasia in the base of secondary gill lamellae and hemorrhages in the capillaries. Some cells in the proliferated interlamellar epithelia exhibited marginal hyperchromatosis. And severe vacuolated changes in the parenchymal cells and congestion in the central veins were observed in the liver. The specific amplicon (396 bp) was detected from gills and opercula of affected eel PCR using Anguillid herpesvirus-1 (AngHV-1) -specific primer sets HVAPOLVPSD (5-``GTG TCG GGC TTT GTG GTG C-3``) and HVAPOLOOSN (5``-CAT GCC GGG AGT CTT TTT GAT-3``). Sequencing analysis of the amplicon demonstrated that this gene was 99% homologous to the AngHV-1 sequence deposited in GenBank. This is the first report of AngHV-1 outbreak in the farmed shortfin eels (A. bicolor) in Korea. When diseased fish were maintained for 10 days at water temperatures of 32℃ and 35℃, the cumulative mortalities were 100% and 10%, respectively. Even though the AngHV-1 genome in the gills from the eel kept at 35℃ was detected using PCR, the structure of gill filaments was similar with that of normal fish. Increasing the water temperature to 35℃ was an effective way to diminish the mortality of AngHV-1 affected eel.
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자연산 홍가자미 (Hippogossoides dubius) 의 피부 종양의 조직학적 관찰
박성우 ( Sung Woo Park ),유진하 ( Jin Ha Yu ),정은빈 ( Eun Bin Jung ),송재희 ( Jae Hee Song ) 한국어병학회 2012 한국어병학회지 Vol.25 No.3
2009년 1월 6일경 어청도∼고군산군도 사이에서 채낚기로 어획되어 군산수협 위판장에서 입수한 홍가자미의 피부 유두종을 병리조직학적으로 검사한 결과 저서성 어류에 발생되고 있는 상피성 유두종으로 종양의 내부는 특징적인 x-cell로 구성되어 있었다. 그러나 x-cell의 핵내의 특징인 대형의 핵인은 시료 채취 후의 자가 분해에 의한 탓인지 관찰할 수 없었다. Histopathological observations on a epidermal papilloma of wild red halibut (Hippogossoides dubius) caught at the area around Echung Island and Gogunsan Islands were done. The papilloma formed on the dorsal fin was the same color with the body on the ocular side but black on the non-ocular side. Under light microscopy, the tumor on the skin was very similar to epithelial papilloma supported by connective tissue. X-cell-like cells in the tumors were large and eosinophilic with a centrally-located, large nucleus. But the characteristic large nucleolus in the nucleus of the x-like cells was indistinct clear in this specimen because about 6-9 hours have passed after catching the fish and autolysis have occurred.
ScienceON
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