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치이즈로부터 분리된 유용 박테리오신 생산균주의 미생물학적 특성 및 발효조에서의 박테리오신 생산
정민용,백현동 경남대학교 환경문제연구소 1999 환경연구 Vol.22 No.-
Bacteriocins are natural antimicrobial compounds produced by many microorganims associated with foods, so that there is currently much interest in their use as food biopreservatives. Goal of this study were to identify bacteriocin-rpoducing strain and to produce the bacteriocin in jar fermenter. Lactic acid bacteria JW3 was tentatiely identified as Lactococcus lactis by the use API 50 CHL kit. Therefore, lacticin JW3 is proposed as the tentative name of the bacteriocin produced by Lactococcus lactis JW3. Lactitin JW3 production in jar fermenter was reached the maximum at the early stationary phase, but decreased after the stationary phase. The bacteriocin showed typical primary metabolite kinetics.
정민용,임형택,백현동 경남대학교 환경문제연구소 2000 환경연구 Vol.23 No.-
In order to select probiotics with a high survival rate in gut and the growth inhibition of virulent pathogens to human beings or animals, we have examined a variety of microorganisms to assess the artificial gastric acid and bile acid. In addition, microbiological identification, antimicrobial activity, enzyme activity, and antibiotic resistance were performed. The selected strains survived for 24 h in artificial bile after incubation for 2 h in artificial gastric juice. Especially, MY4 strain showed higher survival for 2 h incubation in artificial gastric juice. As a result of API CHL 50 kit test, strains MY4, PT77, and PS-490 were tentatively identified as Lactobacillus plantarum, while strain PS653 was tentatively identified as Lactococcus lactis. All lactic acid bacteria were shown to inhibit the growth of Listeria monocytogenes ATCC 19111 dramatically within 24 h of incubation. In test of API ZYM kit, β-glucuronidase was not produced by any kind of lactic acid bacteria. All strains were resistant to antibiotics such as nisin, tetracycline, streptomycin, and rifamycin.
비스판 변이주인 Bacillus polyfermenticus KD21의 주요 생균제 특성
정민용,정황영,하정욱,백현동 慶南大學校 附設 基礎科學硏究所 2002 硏究論文集 Vol.16 No.-
Bacillus polyfermenticus SCD which is commonly called as Bispan strain are being used for functional foods using the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine in both humans and animals. B. polyfermenticus KD21 was obtained for higher sporulation through the NTG mutagenesis. The cells of B. polyfermenticus KD21 was treated for 24 h in artificial bile after incubation for 2 h in artificial gastric juice and final number of the strain was reached to around 10^8 CFU/㎖. In test of API ZYM kit, β-glucuronidase or β-glucosidase were not produced by B. polyfermenticus KD21. B. polyfermenticus KD21 was resistant to antibiotics such as nisin, tetracyclin, streptomycin, and rifamycin. B. polyfermenticus KD21 was shown to inhibit the growth of Listeria monocytogenes ATCC 19111 completely within 24 h of incubation, which indicates its bactericidal nature.