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서민구 ( Min Goo Seo ),장영술 ( Young Sul Jang ),이은미 ( Eun Mi Lee ),박노찬 ( No Chan Park ),곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.2
This study was conducted to determine the prevalence of antibodies to Toxoplasma gondii (TG) in cattle and pigs reared in eastern areas of Gyeongbuk province by ELISA. Among 368 sera collected from 119 cattle farms, 76 (20.7%) sera from 34 (28.6%) farms had antibodies to TG. Fifty (27.2%) out of 184 cattle in Uljin-gun and 26 (14.1%) out of 184 cattle in Yeongdeok-gun were positive. Pyeonghae (50.0%) in Uljingun and Dalsan (33.3%) in Yeongdeok-gun had the highest TG antibodies in cattle compared to other areas. Prevalence of TG antibodies in cattle was increased with age. Among 368 sera collected from 43 pig farms, 62 (16.8%) sera from 16 (37.2%) farms had antibodies to TG. Forty (21.7%) out of 184 pigs in Uljin-gun and 22 (12.0%) out of 184 pigs in Yeongdeok-gun were positive. Uljin and Puk (40.0%) in Uljin-gun and Yeonghae (33.3%) in Yeongdeok-gun had the highest TG antibodies in pigs compared to other areas. Prevalence of TG antibodies in sows was higher than that in fattening pigs. Seasonally, prevalence of TG antibodies in pigs was highest in summer (23.4%) and lowest in winter (12.5%). Based on these observations, data indicate that infection by the protozoan parasite TG is widely prevalent in cattle and pigs reared in eastern areas of Gyeongbuk province.
울진군 한우 Neospora caninum 감염 실태 조사
전령훈 ( Ryoung Hoon Chon ),장영술 ( Young Sul Jang ),이은미 ( Eun Mi Lee ),최정혜 ( Jung Hye Choi ),박노찬 ( No Chan Park ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.3
This study was carried out to investigate an epidemiological state of neosporosis in Korean indigenous cattle in Uljin. Bloody samples were collected from 552 female cattle (337 farms), more than 5 years old, in 10 districts of Uljin. Antibody to N caninum were examined by enzyme linked immunosorbent assay (ELISA). Seroprevalence of individual and farm were 7.6%(42/552) and 8.6%(29/337), respectively. Positive rates by districts was variable (0%~14.9%), and it was seemed to be a tendency that positive farms gathered with in a small zone. In seroprevalence by herd size farms having between 5-9 heads was top(37%), but there was no significant difference among herd size.
이선미 ( Seon Mi Lee ),김미숙 ( Mee Sug Kim ),장영술 ( Young Sul Jang ),전령훈 ( Ryoung Hoon Chon ),박노찬 ( No Chan Park ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.2
Johne`s disease is a chronic inflammatory bowel disease of cattle, sheep, goats and other ruminants, and Mycobacterium paratuberculosis is the etiologic agent of this disease. Many studies have been carried out on paratuberculosis from daily cattle and Korean native cattle in multiple areas around nation, but there is no report in Eastern-Gyeongbuk area. The purpose of this study is to investigate the seroprevalence of bovine paratuberculosis in Eastern-Gyeongbuk area. From July to December in 2007, blood samples were collected from 363 dairy cattle of 27 farms and 281 Korean cattle of 114 farms and the ELISA was conducted. 25 (6.9%) dairy cattle of 6 (22%) farms and 19 (6.8%) Korean cattle of 8 (7.0%) farms were positive in ELISA. In regional analysis, 25 (8.3%) out of 300 dairy cattle in Gyeungju were positive and Pohang were negative in this research. 12 (16.4%) out of 73 Korean cattle in Gyeungju and 7 (9.6%) out of 73 Korean cattle in Uljin were positive. Pohang and Youngdeok of Korean cattle were negative in this research. According to raising scale of dairy cattle, 4 (66.7%) farms out of 6 farms were raising 30 below and 2 (33.3%) farms out of were raising 30~70. And there were negative raising scale more than 70. In Korean cattle, 6 (75%) farms out of 8 were raising below 10 and 2 (25%) farms were raising 10~30. And there were negative raising scale more than 30. The rate of seropositive of paratuberculosis dairy cattle and Korean cattle were similar and the positive rate of Eastern-Gyeongbuk area is reported lower than that of any other region.
PFGE를 이용한 경북지역에서 분리된 Brucella abortus의 유전형별
조민희 ( Min Hee Jo ),김성국 ( Seong Guk Kim ),김영환 ( Young Hoan Kim ),김순태 ( Soon Tae Kim ),엄현정 ( Hyun Jung Eom ),장영술 ( Young Sul Jang ),고영활 ( Young Hwal Ko ) 한국가축위생학회 2009 韓國家畜衛生學會誌 Vol.32 No.3
Subtyping of Brucella abortus isolates is epidemiologically important for monitoring of bovine brucellosis outbreaks. Pulsed-field gel electrophoresis (PFGE) is considered as a gold standard of molecular typing methods to study the DNA polymorphisms of bacteria. In this study, we analyzed using PFGE the DNA fragment profiles of B. abortus isolated in Gyeongbuk province from 1998 to 2006. The genomic DNA was digested with the restriction endonuclease Xba I, Xho I and Smi I followed gel electrophoresis. No distinguishable patterns of the genomic DNA digested with Xba I and Xho I were observed among the field isolates of B. abortus tested in this study. But Smi I restriction enzyme resulted in two PFGE patterns consisting of 13-15 bands that ranged in size from 33 to 668bp by standard marker. The cluster analysis by DNA fingerprinting software showed 93.75% similarity between two PFGE patterns. No different PFGE patterns were recognized among the isolates originated from various years, regions and cow breeds.
경북지방 개 브루셀라병 다두 발생농장의 혈청학적 및 세균학적 조사
김성국 ( Seong Guk Kim ),서희진 ( Hee Jin Seo ),김순태 ( Soon Tae Kim ),장영술 ( Young Sul Jang ),조민희 ( Min Hee Jo ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2
Canine brucellosis is a contagious disease of the reproductive tract that cause mainly abortion and infertility in dog. A serological and bacteriological survey was conducted for breeding kennels which were suffered from frequent outbreak of canine brucellosis in Gyeongbuk province in 2009. Among 138 samples, 45 serum samples were sero-positive. Brucella canis was isolated from 30 blood samples of the seropositive cases, and from 2 samples of 62 sero-negatives. The biochemical properties of 32 isolates were characterized with no production of H2S, no fermentation of carbohydrates, hydrolyzation of urea, and development of thionin dye medium. At amplification of BCSP and 16S-rRNA gene using PCR, 711bp and 905bp DNA fragments were detected in agarose. Three tandem repeat pattern was shown in genotyping by Multi-locus VNTR assay (MLVA).
경북지방 환돈에서 분리한 Salmonella Typhimurium의 생화학적 성상 및 약제내성 패턴
김성국 ( Seong Guk Kim ),엄현정 ( Hyun Jung Eom ),김순태 ( Soon Tae Kim ),장영술 ( Young Sul Jang ),조민희 ( Min Hee Jo ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.1
Salmonella Typhimurium is a virulent pathogen for human and animal. We studied serotypes, biochemical characteristics, and antimicrobial resistance of S. Typhimurium isolated from diseased pigs in Gyeongbuk province over 1998 to 2008. One hundred sixteen isolates were identified as S. Typhimurium by biochemical characteristics and serotypes from 90 farms. The biochemical characteristics of S. Typhimurium isolates was production of H2S, indole-negative, fermentation of mannitol, dulcitol, sorbitol, inositol, rhamnose, and maltose, and ornithine decarboxylase. At antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, cefepime, ciprofloxacin, while were highly resistant streptomycin, cephalothin, enrofloxacin, nalidixic acid, apramycin, chloramphenicol, tetracycline. The isolates were divided into 65 resistant patterns and 47 of the isolates were shown as a DT104 ASSSuT resistant phenotype.
경북지역 개에서 분리된 Brucella canis의 MLVA-16 typing
김성국 ( Seong Guk Kim ),손준형 ( Joon Hyung Sohn ),장영술 ( Young Sul Jang ),김상윤 ( Sang Yoon Kim ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.4
Canine brucellosis is the zoonosis in worldwide and Brucella (B.) canis is a facultative intracellular pathogen that has a very limited host. MLVA-16 (Multilocus VNTR analysis) is a efficient method for genotyping of Brucella species. Various methods have been established for genotyping of Brucella species, but most of analytical method is lack reproducibility and limited capability to differentiate them. B. canis isolates (n=73) from 7 farms in Gyeongbuk province in 2003∼2010 were analyzed using 16 VNTR loci. Automatic electro-phoresis system was utilized for more high throughput and rapid simple discrimination. Thirty two genotypes were identified from 73 B. canis isolates. MLVA could contribute to molecular typing for epidemiological evaluation of canine brucellosis.
우인옥 ( In Ohk Ouh ),서민구 ( Min Goo Seo ),장영술 ( Young Sul Jang ),김상윤 ( Sang Yun Kim ),곽동미 ( Dong Mi Kwak ) 한국동물위생학회 2013 韓國家畜衛生學會誌 Vol.36 No.4
This study was done to determine the seroprevalence of Coxiella burnetii in cattle with reproductive disorders reared in eastern Gyeongbuk province. A total of 146 cattle blood samples from 83 farms in eastern Gyeongbuk province were collected in 2011∼2012. Among them, 17 (11.6%) samples from 11 (13.3%) farms were seropositive for C. burnetii by ELISA. In dairy cattle with mastitis, 15 (38.5%) milk samples from 9 (42.9%) farms were seropositive among 39 milk samples from 21 farms. Among the seropositive farms, one farm with high seroprevalence was selected to assess the seroprevalence of C. burnetii in the bulk-tank milk (BTM) and blood samples of dairy cattle. The BTM sample from the farm was seropositive to C. burnetii and 6 (15%) out of 40 dairy cattle were seropositive. In cattle with reproductive disorders including infertility, abortion and stillbirth, 2 (1.9%) samples from 2 (3.2%) farms were positive among 107 samples collected from 62 farms. Among 2 positive samples, one of them is dariy cattle (100%) and the other is Korean cattle (0.9%) (P<0.001). This suggests that Korean cattle was relatively resistant to C. burnetii. Based on the data obtained, we identified association between C. burnetii shedding in milk and chronic subclinical mastitis in dairy cattle. Since seroprevalence of C. burnetii is relatively high in dairy cattle, further intensive studies on C. burnetii among the high risk milk farms and dairy cattle herds are needed to evaluate infection status.