고추의 풋마름병청고병(靑枯病)에 대한 저항성

임양숙,김병수 한국식물병리학회 1994 Plant Pathology Journal Vol.10 No.2

감과원 총채벌레류의 발생상황 및 약제선발

임양숙,조두현,서동환,양태식 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

최근 이상기후로 인해 매년 감과원내 총채벌레류의 발생이 증가하고 있으며 이들 중 가장 경제적으로 피해를일으키는 피해를 일으키고 있는 종류는 볼록총채벌레(Scirtothrips dorsalis), 감관총채벌레(Ponticulothrips diosprosi)이다. 2016년부터 2년간 경북지역 감과원에서 발생되는 총채벌레류 발생변화를 조사하였으며 볼록총채벌레에 대한우수한 방제약제를 선발하였다. 황색끈끈이트랩을 이용하여 조사한 결과, 볼록총채벌레, 감관총채벌레, 대만총채벌레, 꽃노랑총채벌레, 하와이총채벌레 등이 조사되었으며 총채벌레 5종 모두 2016년 보다 2017년의 발생량이 많았다.이들 중에 감나무에 심각한 피해를 일으키는 볼록총채벌레와 감관총채벌레에 대한 지역별 발생조사에서 볼록총채벌레는 청도지역이 상주지역보다 발생밀도가 높았고 주 발생시기도 5월중순~7월상순으로 다소 빠른 경향이었다.감관총채벌레는 상주지역의 발생밀도가 높았고 주 발생시기는 5월하순~8월상순이었다. 볼록총채벌레 방제를 위한약제선발 시험에서 클로르페나르피르 액상수화제 등 4약제 모두 90% 이상의 방제가를 나타내어 감 볼록총채벌레방제에 우수한 효과를 나타내었다.

Phytophthora nicotianae에 의한 일일초 역병

임양숙,최충돈,김병수 한국식물병리학회 2004 식물병연구 Vol.10 No.1

A disease causing blights on leaves, stems, flowers, and pods of Vinca (Catharanthus roseus L.) was occurred in Aug. 2003 in Chengdo Peach Experiment Station. A species of Phytophthora was isolated from the diseased tissues. The causal fungus was identified as Phytophthora nicotianae on the basis of mycological characteristics and pathogenicity. Sporangia were ovoid to spherical, noncaducous, prominently papillate and averaged 38.0 × 31.0 mm in dimension. Chlamydospores were bundantly produced on agar media and sized about 30.5mm in diameter. The fungus was heterothallic and A1 mating type. Oospores were measured 23.1 mm in size. Optimum temperature for growth of the fungus was 25 to 30oC. This is the first report of occurrence of foliage blight of Vinca caused by P. nicotianae in Korea.

고추 갈색점무의병원균(Cercospora capsici)의 배양특성과 다량 포자형성법

임양숙,김병수 한국식물병리학회 2003 식물병연구 Vol.9 No.3

High sporulation method and cultural characteristics of Cercospora capsici causing Cercospora leaf spot of pepper were examined. Optimum temperature for mycelial growth of Cercospora capsici was 25oC. The fungus did not grow below 5oC and over 35oC. Optimum pH for mycelial growth was pH 4.0~pH 8.0. Mycelial growth was not influenced by light. C. capsici sporulated well on pepper leaf agar (5 g/l). A standard method of sporulation established was as follows. The mycelial plugs were ground with some water in motar with pestle. The mycelial suspension was smeared on the surface of medium and incubated for 2~3 days at 20oC. The culture surface was lightly scraped with a brush after adding 1 ml of sterile water to stimulate sporulation and further incubated for 2~3 days.

Phytophthora nicotianae와 P. palmivora에 의한 대추역병 발생

임양숙,정기채,김승한,윤재탁 한국식물병리학회 2002 식물병연구 Vol.8 No.1

Two species of Phytophthora were isolated from infected fruits of jujube. Among 18 isolates collected, 6 were identified as P. nicotianae and 12 as P. palmivora on the basis of their mycological characteristics. The former produced no caducous, ovoid to spherical sporangia in contrast to caducous, ellipsoid, and broadly ovoid to spherical ones of the latter. These two species were shown to be heterothallic and markedly papilate, chlamydospores abundant, and A1 mating type. They showed strong pathogenicity to fruits of jujube and pear while no symptom was produced on apple fruit by artificial inoculation. This is the first report of jujube fruit rot caused by P. nicotianae and P. palmivora in Korea.

저항성 검정을 위한 고추 갈색점무늬병(Cercospora capsici) 최적 발병조건

임양숙,김병수 한국식물병리학회 2003 식물병연구 Vol.9 No.3

Occurrence of cercospora leaf spot by Cercospora capsici is assamed to remarkbly cause the yield reduction through the leaf spot, yellowing of leaves and early defoliation in pepper cultivation. To develop resistant cultivar, optimal screening conditions of pepper against cercospora leaf spot were studied. Optimal screening conditions were 3.4 × 105 conidia/ml conidial suspension were sprayed on to the leaves of 30~40 days old pepper seedlings, incubated at 15~20oC/20~25oC (day/night) and examined 20 days after inoculation.

고추 갈색점무늬병원균 (Cercospora capsici)의 배양특성과 다량 포자형성법

임양숙,김병수,Lim Yang-Sook,Kim, Byung-Soo 한국식물병리학회 2003 식물병연구 Vol.9 No.3

High sporulation method and cultural characteristics of Cercospora capsici causing Cercospora leaf spot of pepper were examined. Optimum temperature for mycelial growth of Cercospora capsici was $25^{\circ}C$. The fungus did not grow below $5^{\circ}C$ and over $35^{\circ}C$. Optimum pH for mycelial growth was pH 4.0~pH 8.0. Mycelial growth was not influenced by light. C. capsici sporulated well on pepper leaf agar (5g/l). A standard method of sporulation established was as follows. The mycelial plugs were ground with some water in motar with pestle. The mycelial suspension was smeared on the surface of medium and incubated for 2~3 days at $20^{\circ}C$. The culture surface was lightly scraped with a brush after adding 1 ml of sterile water to stimulate sporulation and further incubated for 2~3 days. 고추 갈색점무늬병(Cercospor capsici)병원균의 균사특성과 포자형성 방법 조사하였다. C. capsici 병원균의 균사생장 적온은 $25^{\circ}C$이였고 $5^{\circ}C$ 이하와 $35^{\circ}C$ 이상에서는 균사생장이 일어나지 않았다. pH 4.0~pH 8.0의 넓은 범위에서 균사생장에 차이가 없었고 일장도 균사생장에는 영향을 주지 않는 것으로 나타났다. 분생포자 다량 형성 시키기는 배지로는 0.5% 고추잎배지가 가장 좋았으며 배지 상에서 포자를 다량으로 형성시키는 방법은 고추잎배지에 균사현탁액을 도말 한 후 2,3일간 $20^{\circ}C$에서 배양한 후 1ml/접시 살균수를 첨가하여 배지표면을 긁어 자극을 준 후 2, 3일정도 배양을 하면 포자형성이 양호하였다.

감과원 잡초별 볼록총채벌레 발생상황 변화

임양숙,조두현,서동환,양태식 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

최근 감과원내 볼록총채벌레(Scirtothrips dorsalis)의 발생으로 과실 표면에 상처가 생기고 회갈색으로 코르크화되는피해가 발생되어 과실 생산에 경제적 피해를 주고 있다. 볼록총채벌레에 대한 월동상황을 조사하기 위해 과원내분포하는 식물을 대상으로 2016년 3월부터 9월까지 조사하였다. 감과원내 잡초에서 발생되는 총채벌레류는 볼록총채벌레, 감관총채벌레, 대만총채벌레, 꽃노랑총채벌레, 하와이총채벌레였으며 발생밀도가 가장 높은 종은 대만총채벌레였다. 감과원 잡초내 볼록총채벌레의 충태별 발생상황조사에서 월동성충은 4월상순부터 발생하기 시작하였고 4월하순, 5월중순, 6월하순, 7월중순, 8월중순의 5회 발생최성기를 나타내었다. 유충은 4월중순 초기 밀도가 높았으며6월상순, 6하순, 7월중순 8월중순의 4회 발생 피크를 나타내었다. 볼록총채벌레의 발생 밀도가 높은 잡초류는 켄터키블루그라스, 털별꽃아재비, 클로버, 쑥, 가박사리 속속이풀, 별꽃 등이었으며 월동 후, 4월부터 켄터키블루그라스,별꽃, 클로버, 쑥 등에서 발생되었으며 5월부터는 소리쟁이, 털별꽃아재비 등에서 발생되었다. 6월부터는 쇠비름,메꽃 등에서 발생되었다. 이 중, 별꽃, 켄터키블루그라스, 클로버, 쑥, 털꽃아재비 등에서는 감 생육기간 동안 볼록총채벌레가 계속 발생되었다. 감과실에 피해가 일어나기 시작하는 5월중순부터 6월하순까지는 과원 잡초와 감꽃, 감과실사이를 옮겨가며 피해를 주고 있었다.

깨어나 꼿꼿이 일어설 수 있는 여성이길 외치며

임양숙 한국마케팅연구원 1992 마케팅 Vol.26 No.7

가지의 Ralstonia solanacearum에 의한 풋마름병 발생과 생리형의 분화

임양숙,김병수,이문중,정정도,류영현 한국식물병리학회 2008 식물병연구 Vol.14 No.1

Batcterial wilt caused by Ralstonia solanacearum is one of important and widespread diseases worldwide as well as in Korea. Bacterial wilt disease caused by R. solanacearum has been reported mainly in solanaceous crops including eggplant (Solanum melongena), tomato (Solanum lycopersicum), potato (S. tuberosum), and pepper (Capsicum annuum). A total of 48 strains of R. solanacearum from eggplant were collected during 2005 and 2006. They were confirmed as R. solanacearum by PCR amplification with primer pair flipcF/flipcR resulting in production of 470-bp DNA fragment. The 15 isolates exhibited pathogenicity on eggplant and tomato, but less virulent on pepper than other species. The biovar of collected isolates, which have been reported of five types worldwide, were classified as biovars 3 and 4 by physiological test. Biovar 4 was the dormant type without pathogenicity on eggplant rootstock, whereas biovar 3 had pathogenicity on eggplant rootstocks that is resistant to R. solanacearum, indicating necessity of breeding new rootstock with resistance to R. solanacearum biovar 3 Batcterial wilt caused by Ralstonia solanacearum is one of important and widespread diseases worldwide as well as in Korea. Bacterial wilt disease caused by R. solanacearum has been reported mainly in solanaceous crops including eggplant (Solanum melongena), tomato (Solanum lycopersicum), potato (S. tuberosum), and pepper (Capsicum annuum). A total of 48 strains of R. solanacearum from eggplant were collected during 2005 and 2006. They were confirmed as R. solanacearum by PCR amplification with primer pair flipcF/flipcR resulting in production of 470-bp DNA fragment. The 15 isolates exhibited pathogenicity on eggplant and tomato, but less virulent on pepper than other species. The biovar of collected isolates, which have been reported of five types worldwide, were classified as biovars 3 and 4 by physiological test. Biovar 4 was the dormant type without pathogenicity on eggplant rootstock, whereas biovar 3 had pathogenicity on eggplant rootstocks that is resistant to R. solanacearum, indicating necessity of breeding new rootstock with resistance to R. solanacearum biovar 3