한국재래산양 췌장의 insulin, glucagon, somatostatin 및 pancreatic polypeptide 분비세포에 관한 전자현미경적 연구

이흥식,이인세,강태천,원무호,이성준,Lee, Heungshik S.,Lee, In-se,Kang, Tae-cheon,Won, Moo-ho,Yi, Seong-joon 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.1

Ultrastructures of pancreatic endocrine cells containing glucagon, insulin, somatosratin and pancreatic polypeptide were studied in the pancreas of the Korean native goat by immunohistochemical and elecron microscopy. Glucagon immunoreatctive cells were round or fusiform in shape and contained secretory granules of 200-260 nm in diameter. The secretory granules were high in electron density and had a halo between the limiting membrane and the central granule core. Insulin immunoreactive cells were round or oval in shape, and contained various sizes of secretory granules from 135 to 300 nm in diameter. The secretory granules were low or moderate electron density and had a variform halo. Somatostatin immunoreactive cells were elliptical or fusiform shape with cytoplasmic processes. They contained the secretory granules of 140-320 nm with moderate electron densities. Pancreatic polypeptide immunoreactive cells were elliptical or fusiform and contained small secretory granules with high electron densities. The secretory granules were 120-230 nm in diameter and the least in number.

한국재래산양 혀에 관한 형태학적 연구

이흥식,이인세,강태천,Lee, Heungshik S.,Lee, In-se,Kang, Tae-cheon 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2

This studies were carried out to identify the characteristics of the tongue of Korean native goat(Capra hircus) by macroscopy, microscopy and scanning microscopy. Korean native goat had torus linguae, median lingual sulcus, lingual fossa and ventral median fissure but did not have glossoepiglottic fold and terminal sulcus in the tongue. The whole length of tongue was $11.51{\pm}0.76cm$. The length of tongue apex, tongue body, tongue root and the torus linguae were $2.62{\pm}0.28$, $7.39{\pm}0.27$, $1.56{\pm}0.26$ and $6.37{\pm}0.29cm$, respectively. The width of tongue apex, torus linguae and tongue root were $3.41{\pm}0.24$, $3.74{\pm}0.29$ and $3.68{\pm}0.11$, respectively. The thickness of tongue apex was $1.60{\pm}0.10$, and the height of torus linguae was $1.52{\pm}0.15cm$. Filiform papillae were present at the tongue apex and the tongue body rostral to torus linguae. Fungiform papillae were scattered from tongue apex to rostral portion of torus linguae, being in abundance at the tongue apex. Vallate papillae were showed at the lateral portion of torus linguae, while lentiform papillae were present at its central portion. Conical papillae were located between vallate and lentiform papillae. The numbers of filiform, fungiform, conical, vallate and lentiform papillae were $46,980{\pm}1070.98$, $446.8{\pm}36.97$, $818.4{\pm}43.99$, $34.8{\pm}2.77$, and $255.6{\pm}39.30$, respectively. The average numbers of taste bud were $8.3{\pm}2.04$ in a fungiform papilla and $247.3{\pm}37.44$ in a vallate papilla. The filiform papilla had secondary and tertiary papillae. The height of filiform papilla was about $150{\mu}m$ and the diameter was $100{\mu}m$. The diameters of fungiform papillae were 350 to $550{\mu}m$. The long and short diameters of maximum-sized lentiform papilla were 4000 and $3000{\mu}m$, respectively, while those of minimum-sized papilla were 700 and $600{\mu}m$, respectively. The height of conical papillae was 450 to $600{\mu}m$ and diameter was 250 to $450{\mu}m$. The vallate papilla was round or oval in shape and its diameter was 500 to $850{\mu}m$. It had well-developed papillary groove around itself. The modified conical papillae were not observed in the tongue of Korean native goat.

한국재래산양 혀에 분포하는 신경전달물질에 관한 면역조직화학적 연구

이흥식,이인세,강태천,Lee, Heungshik S.,Lee, In-se,Kang, Tae-cheon 대한수의학회 1996 大韓獸醫學會誌 Vol.36 No.2

This study was performed to identify the localization of several neuropeptides; calcitonin gene-related peptide(CGRP), substance P(SP), vasoactive intestinal polypeptide(VIP), neuropeptide Y(NPY), serotonin(5-HT) and neurotensin in the tongue of Korean native goat(Capra hircus) by immunohistochemical method. The results were summarized as follows: CGRP- and SP-immunoreactive fibers were observed as moderate immunoreactivity at the subepithelial plexus and subgemmal fibers in lamina propria of lingual papillae, but not seen in intragemmal, intergemmal, perigemmal fibers as well as in the supporting, basal and taste cells. Fibers around the acinus of the von Ebner's gland and blood vessels showed weak immunoreactivities against CGRP and SP. In the intrinsic ganglion cells, CGRP- and SP-immunoreactivities were not observed. The distribution patterns of VIP- and NPY-immunoreactive fibers were similar to CGRP-or SP-immunoreactive fibers, but their immunoreactivities were stronger than those of CGRP- or SP immunoreactive fibers. The immunoreactivities to VIP or NPY were seen in the intrinsic ganglion. Only a few serotonin immunoreactive fibers were seen in some filiform or fungiform papillae. Neurotensin immunoreactivity was not observed in the tongue of Korean native goat.

한국재래산양 췌장의 insulin, glucagon, somatostatin 및 pancreatic polypeptide 분비세포에 관한 면역조직화학적 연구

이흥식,이인세,강태천,김진상,이성준,Lee, Heungshik S.,Lee, In-se,Kang, Tae-cheon,Kim, Jin-sang,Yi, Seong-joon 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.1

Pancreatic endocrine cells containing glucagon, insulin, somatostatin and pancreatic polypeptide were identified in the pancreas of the Korean native goat by using immunohistochemical method. Glucagon immunoreative cells were oval or fusiform in shape and located at the periphery of the pancreatic islets. Insulin immunoreactive cells were round or oval in shape and occupied throughout the pancreatic islets except the small area of the periphery. Somatostatin immunoreative cells were oval and elliptical, and mainly located at the periphery of the pancreatic islets. Some of these cells had a cytoplasmic process. Pancreatic polypeptide immunoreactive cells were elliptical or polyhedral and located at the periphery of the pancratic islets where two or more cells formed a cell cluster. The distribution rates of glucagon, insulin, somatostatin and pancreatic polypeptide immunoreactive cells were 24.4%, 44.3%, 13.2% and 18.1% respectively.

한국재래산양 송과체와 앞쪽목신경절의 관계규명을 위한 면역조직화학적 연구

이흥식,이인세,송승훈,윤성태,황인구,이충현,Lee, Heungshik S.,Lee, In-Se,Song, Seung-hoon,Yoon, Sung-tae,Hwang, In-koo,Lee, Choong-hyun 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.2

The pineal body have been known to be affected by superior cervical ganglia, and most of its nerve fibers containing peptidergic neurotransmitters have been considered to be originated from this ganglia. To confirm this relationships, some peptidergic neurotransmitters were identified in both of pineal body and superior cervical ganglia of the Korean native goat, which were divided into two group; breeding season and non-breeding season. The localizations of two catecholamine-synthesizing enzymes; tyrosine hydroxylase (TH) and dopamine beta-hydroxylase (DBH), were investigated by immunohistochemistry in the superior cervical ganglia and the pineal body of adult Korean native goats. Substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and galanin (GAL) were also identified in these organs by immunohistochemical and double immunofluorescent methods. In superior cervical ganglia, immunoreactivities for TH and DBH were confirmed in the same ganglion cells. The immunoreactivites for SP, VIP(only in male), NPY and GAL were identified in both of ganglion cell bodies and nerve fibers in the ganglia. CGRP immunoreactivity, however, was observed only in nerve fibers. Most NPY- and VIP-immunoreactive(IR) ganglion cells also contained TH. SP and TH were colocalized in the cell bodies, but not in the nerve fibers. TH immunoreactivity was shown in almost all of ganglion cells in the superior cervical ganglia. The immunoreactivity for NPY had some seasonal variation and was stronger in breeding season than in non-breeding season. In pineal body, lots of TH-IR fibers were observed throughout the parenchyma including the pineal stalk and most of them also contained DBH. SP- and NPY-IR fibers were also immunostained with TH or DBH. But a few SP- and NPY-IR fibers were not colocalized with TH or DBH. Exceptionally, a bipolar neuron-like cell was observed to be immunostained with NPY in the pineal body. A few CGRP and GAL-IR fibers were observed, while VIP-IR fibers were not present. It is concluded that most TH- and DBH-IR fibers as well as the peptidergic immunoreactive fibers of the pineal body might be originated from the superior cervical ganglia. Some peptidergic immunoreactive fibers, however, might be come from other regions of brain. We also suggest that NPY in pineal body plays a important role for pineal function. The seasonal variation of NPY immunoreactivity indicates that the synthesis and use of NPY may be different between in breeding and non-breeding seasons.

방사성동위원소의 이용과 실제

이흥식,Lee Heungshik 대한수의사회 1979 대한수의사회지 Vol.15 No.9

한국재래산양 하악골에 관한 해부학적 연구

이흥식,이성준,Lee, Heungshik S.,Yi, Seong-joon 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.3

This study was carried out to identify metric and non-metric characteristics of the mandible of the Korean native goat. The results were summarized as follows : The length of the mandible was more correlated with the width of the base of the ramus mandibulae than the margo alveolaris(p<0.01). The height between incisura vasorum facialium and processus condylaris had very high correlation with the height between incisura vasorum facialium and incisura mandibulae(p<0.01). The former was also more correlated to the height of the mandible in front of the first molar tooth than the height of the mandible behind the last molar tooth(p<0.01). The symphysis was formed between both mandibles and it was not able to observe ossification in all case. The number of alveoli for incisor teeth were four each mandible, however three alveoli were observed in two cases. There was usually one or occasionally, two foramina mentalia on the lateral surface of the corpus mandibulae below the margo interalveolaris near the symphysis. The incisura vasorum facialium was poorly developed. A distinct groove was observed rostroventral to the foramen mandibulae. The processus condylaris was shown hammer shape, and the caput mandibulae was flat rectangular form. The processus coronoideus was formed a rectangle and curved laterally. The incisura mandibulae was well developed.

일반사육시설 마우스의 유전적 오염에 대한 실태조사 연구

이흥식,김철규,Lee, Heungshik S.,Kim, Chul-kyu 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3

These studies were carried out to survey the genetic contamination of six inbred mice (A, BALB/c, C3H, C57BL/6, CBA and KK) produced and supplied from the conventional breeding unit for improving the quality of mice as experimental animal. We examined alleles of five loci (Akp-1, Car-2, Hbb, Es-1 and Trf) by the use of biochemical markers with celluose acetate electrophoresis. As the results of test, BALB/c, A, C3H, C57BL/6, CBA and KK showed standard alleles in Akp-1, Car-2 and Hbb. But Es-1 of A and C57BL/6 and Trf of A, C3H, C57BL/6 and CBA did allelic divergence in loci. These results suggest that the colonies of A, C3H, C57BL/6 and CBA were genetically contaminated. Therefore, we recommend to eliminate the genetically contaminated mice thoroughly, to check on genetic monitoring regularly and to consider a counterpaln for improving the quality control as soon as possible.

일반시설에서 사육되는 마우스의 품질향상을 위한 기초조사 연구

이흥식,성노현,김경진,김철규,Lee, Heungshik S.,Seung, Rho Hyun,Kim, Kyungjin,Kim, Chul-kyu 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.3

For the improvement of quality control of laboratory mouse, we investigated the environmental condition, histopathological findings and serological test using ELISA to mouse hepatitis virus(MHV), Mycoplasma pulmonis(MP), Clostridium piliforme(TZ) and Sendai virus (HVJ) of ICR, C57BL/6, CBA and C3H/He mice that were supplied from conventional laboratory animal facility. 1. The ammonia concentration of facility was below the recommended concentration, 15ppm, by the KNIH, and the room temperature($21{\sim}23^{\circ}C$) and relative humidity(40~60%) was optimum range recommend by the Ministry of Health and Welfare, respectively. 2. The incidence rate of inapparent disease was 86.6% and the major findings in the liver were vacuolar degeneration with nucleic pleomorphism. The lung was shown the thickening of alveolar wall and interstitial pneumonia with congestion. The kidney and spleen were observed the mild congestion and extramedullary hematopoiesis, respectively. 3. The positive reaction rates against MHV and MP in serological test was 97.9% and 37.5%, respectively but HVJ and TZ were negative. These results suggest that laboratory mice could be infected with MHV and MP under conventional environments. Therefore we recommend to select thoroughly inapparent infected mice and to convert conventional system into SPF facility as soon as possible.

일반사육시설 마우스의 잠재질병 조사연구

이흥식(Heungshik S. Lee),이영순(Yong Soon Lee),이정주(Chung Choo Lee),정기성(Gey Seong Jeong) 한국실험동물학회 1994 Laboratory Animal Research Vol.10 No.1