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낙동강 수계 폐수배출업소를 대상으로 휘발성유기화합물질 배출실태 조사
이인정 ( In Jung Lee ),임태효 ( Tae Hyo Lim ),허성남 ( Seong Nam Heo ),이재관 ( Jaeg Wan Lee ),이용희 ( Yong Hee Lee ),이호열 ( Ho Yul Lee ),천세억 ( Se Uk Cheon ) 한국환경분석학회 2012 환경분석과 독성보건 Vol.15 No.3
There are many industrial factories in the central Nakdong river basin and have been occurred water pollution accidents by hazardous chemicals such as phenol, 1,4-dioxane and perchlorate. In this study, seven volatile organic compounds, VOCs(1,3-butadiene, vinyl acetate, ethyl acrylate, epichlorohydrin, styrene, hexachloroethane, 1,4-dioxane) potentially discharged into the Nakdong river were determined by purge & trap and gas chromatography-mass spectrometry(GC-MS). Method detection limit(MDL) and limit of quantitation( LOQ) were in the range 0.14~0.38 μg/L and 0.44~1.22 μg/L, respectively. Six compounds(1,3-butadiene, vinyl acetate, ethyl acrylate, epichlorohydrin, styrene, 1,4-dioxane) were detected in wastewater treatment plants(WWTPs) and wastewater discharge facilities from the Nakdong River basin. 1,4-Dioxane was most frequently observed in WWTPs and wastewater discharge facilities.
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이재관 中央大學校 遺傳工學硏究所 1996 遺傳工學硏究論集 Vol.9 No.1
동물세포의 미립담체 배양시 담체표면에 세포 부착되는 속도식을 유출하였고, 속도상수를 구하였다. 부착속도상수는 산소농도의 함수로 표시되었다. 이때 사용되어진 함수 형태는 hyperbolic 형태의 saturation kinetics 였다. 이와 같이 구한 모델 속도식을 실험데이터에 전산 모사한 결과 상당히 잘 적용될 수 있음이 보여졌다. To investigate the attachment kinetics of animal microcarriers hepatocytesliver parenchymal cells-were isolated and cultured with dextran microcarrier. Cytodex 3. The kinetic model assumes the bi-molecular reaction mechanism as follows : free cell surface →k attached cell. The kinetic rate constant was evaluated by fitting the kinetics of the number of attached cells with the model equation using non-linear regressional analysis. And the constant was expressed as a saturation-type function of oxygen concentration in culture medium,k=Vm Co₂/(Km+Co₂). Vm and Km were estimated as 1.26±0.42×10(-5) and 75.6±45.7, respectively. Using the model and the rate constant, computer simulations were performed to evaluate the effect of initial cell concentration and microcarrier concentration, which showed the relatively higher impact of microcarrier concentration on the fraction of attached cells compared to initial cell concentration. Also, the rate equation could simulate the independent experiments of cell attachment kinetics. In conclusion, cell attachment kinetics onto microcarrier was accomplished and the rate constant was determined using non-linear regressional analysis.
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