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공신국 ( Shin Koog Kong ),이건택 ( Gun Taek Lee ),임종묵 ( Jong Mook Lim ),양승민 ( Seung Min Yang ),이요안나 ( Jho An Na Lee ),문순화 ( Sun Hwa Moon ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.2
The purpose of this study was to detect Mycobacterium bovis in cattle(serum, milk, lung, lymph node) by PCR. Nineteen samples from 7 skin test-positive cattle were analyzed. The amplified band of IS6110 by PCR was detected from 2 samples in lung and lymph node. But the sensitivities of the present methods for detecting M bovis in milk and serum are deficient. Because the PCR sensitivity has been shown to he hindered by the method used to isolate the nucleic acid target. PCR-based methods have the potential to be faster, more accurate, and the most efficient means of detecting M bovis. The detection of M bovis by PCR will contribute to the more efficient detection and control of tuberculosis.