농산물 중 살충제 Etofenprox의 잔류분석법

이영득 ( Young Deuk Lee ),모에닌퓨 ( Moe Hnin Phyu ),임무혁 ( Moo Hyeog Im ) 한국환경농학회 2008 한국환경농학회 학술대회집 Vol.2008 No.2

농산물 중 극성 유기인계 살충제의 잔류분석법 개발

이영득 ( Young Deuk Lee ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

An analytical method was developed to determine residues of polar organophosphorus insecticides including azamethiphos, demeton-S-methyl, dicrotophos, oxydemeton-methyl and a major metabolite, demeton-S-methyl sulfone in agricultural products for the inspection of agricultural foods. Total of five representative types of agricultural commodities which comprised brown rice, mandarine, green pepper, potato and soybean were selected for validation of the method. Target analytes were simultaneously extracted with acetone from raw or moistened samples. The extract was diluted with saline water, and subjected to n-hexane washing to remove nonpolar co-extractives while the analyte remained in the aqueous phase. Dichloromethane partition was then followed to recover all the analytes from the aqueous phase. Florisil or weak anion-exchange column chromatography was employed to further purify the extract prior to determination by gas-liquid chromatography (GLC). A temperature-programmed GLC using a polar capillary column was successfully applied to separate and quantitate each residue of the analyte in sample extracts with nitrogen-phosphorus detection. Internal standardization was additionally applied to minimize variation during the instrumental quantitation. A confirmatory and/or alternative quantitative method was also provided using GLC coupled with the mass spectrometric technique.

공작고사리의 기내 포자발아에 미치는 배지의 영향

문영득(Young Deuk Moon),이철희(Cheol Hee Lee) 한국원예학회 2007 한국원예학회 학술발표요지 Vol.2007 No.6

잔류허용기준의 국제적 조화를 위한 작물잔류성 시험 방법 및 자료 해석의 합리화

이영득 ( Young Deuk Lee ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-

A procedural and policy-oriented research was undertaken to fulfill the local maximum residue limits (MRLs) of pesticide residues to the international standards. Scientific basis and criteria for the evaluation of supervised trial dataset to establish local MRLs were precisely compared with MRL calculation systems of Codex, EU and USA, which operated under non-distributional statistical approximations. Practical approach and improvement in pursuit of local implementation was studied to harmonize the local MRL setup system to the international standards in compliance with minimal statistical requirements including number of independent trials, production and handling of residue data along with application of extrapolation cases and food processing factors. Trial guidelines of pesticide residues comprising field and analytical good-laboratory practices (GLPs) were also concisely inspected to produce reliable residue data scientifically applicable to MRL calculation. Manuals with concise standard operating procedures during supervised trials and reporting format for the submission were concurrently provided to readily access the internationally-approved data production.

농산물 중 Methomyl 및 전구체 살충제의 동시 잔류분석법 개발

이영득 ( Young Deuk Lee ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-

An analytical method was developed to simultaneously determine residues of methomyl and its precursor insecticides including alanycarb and thiodicarb in agricultural products for the official purpose. All of target analytes were extracted with methanol from raw or moistened samples. To the extract was added hydrochloric acid to hydrolyze alanycarb. Methomyl was quantitatively evolved from alanycarb within 10 min, while intact methomyl and thiodicarb were stable up to 1 hr in the acidic media. The hydrolyzate was diluted with saline water, and subjected to n-hexane washing to remove nonpolar co-extractives while the analyte remained in the aqueous phase. Enhancing ionic strength of the aqueous phase, dichloromethane partition was then followed to recover methomyl and thiodicarb. Florisil and/or weak anion-exchange column chromatography were employed to further purify the extract prior to determination by high-performance liquid chromatography (HPLC) with detection of ultraviolet absorption at 234 nm. Linear gradient elution of acetonitrile/water mixture on an octadecylsilyl column was successfully applied to separate each residue of the analyte. Total of five representative types of agricultural commodities which comprised brown rice, mandarine, green pepper, potato and soybean were selected for validation of the method. A confirmatory and/or alternative quantitative method were also provided using LC coupled with the mass spectrometric technique.

농산물 중 Sulfonylurea계 제초제의 공정 잔류분석법 개발

이영득 ( Young Deuk Lee ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-

An analytical method was developed to simultaneously determine residues of four sulfonylurea herbicides including chlorimuron-ethyl, ethametsulfuron-methyl, rimsulfuron and tribenuron-methyl in agricultural products for the official purpose. All of target analytes were extracted with acetonitrile containing 0.5 M ammonium chloride from raw or moistened samples. The extract was diluted with saline water, and subjected to n-hexane washing to remove nonpolar co-extractives while the analyte remained in the aqueous phase. Enhancing ionic strength of the aqueous phase, dichloromethane partition was then followed to recover all the analytes. Weak anion-exchange column chromatography using SPE-aminopropyl cartridge was employed to further purify the extract prior to determination by high-performance liquid chromatography (HPLC) with detection of ultraviolet absorption at 234 nm. Linear gradient elution of acetonitrile/phosphate buffer (pH 3.0) mixture on an octadecylsilyl column was successfully applied to separate each residue of the analyte. Total of five representative types of agricultural commodities which comprised brown rice, mandarine, green pepper, potato and soybean were selected for validation of the method. A confirmatory and/or alternative quantitative method were also provided using LC coupled with the mass spectrometric technique.

소형수도재배구중(小型水稻栽培區中) Carbofuran 의 흡수(吸收) 이행(移行) 및 잔류특성(殘留特性)

이영득,박형만,박영선,박창규,Lee, Young-Deuk,Park, Hyung-Man,Park, Young-Sun,Park, Chang-Kyu 한국환경농학회 1987 한국환경농학회지 Vol.6 No.1

수도재배환경중(水稻栽培環境中) carbofuran의 흡수(吸收), 이행(移行) 및 잔류양상(殘留樣相)을 구명(究明)하기 위하여 소형수도재배구(小型水稻栽培區)를 이용(利用), carbofuran 입제(粒劑)를 2수준(水準)으로 살포(撒布)하고 시기별(時期別)로 수질(水質), 수도체(水稻體),토양(土壤) 및 수확물중(收穫物中) 잔류수준(殘留水準)을 조사(調査)한 결과(結果)는 다음과 같다. 수면살포(水面撒布)한 carbofuran 입제(粒劑)는 빠른 속도(速度)로 용출(溶出)되어 수질중(水質中) carbofuran 농도(濃度)는 처리(處理) 1일이내(日以內)에 최고치(最高値)에도 달하였다. 용출(溶出)된 carbofuran은 수도체(水稻體)로 흡수(吸收), 이행(移行)되어 처리(處理) 1∼3일(日) 후(後)에 최고치(最高値)에 도달하였으며 그 이후(以後) 완만한 감소(減少) 경향(傾向)을 보였다. 수질중(水質中) carbofuran의 반감기(半減期)는 4일(日)이었고 토양중(土壤中)에서의 반감기(半減期)는 처리수준(處理水準)에 따라 8∼12일(日)이었다. 수확물중(收穫物中) carbofuran 잔류량(殘留量)은 현미(玄米)의 경우 0.01∼0.02ppm이었으며 볏짚에서는 0.37∼0.07ppm의 범위(範圍)였다. A study has been conducted to investigate the behavior of carbofuran in a miniature paddy agrosystem simulated for paddy field. Carbofuran applied onto the paddy water was rapidly absorbed and translocated into rice plants. Carbofuran concentration in rice plant reached its maximum level between 1 to 3 days after treatment and gradually decreased thereafter. Half life of carbofuran concentration in paddy water was 4 days in both application rates of 0.12 and 0.24Kg a.i./10a. Carbofuran residue in paddy soil was gradually dissipated with the half life of 8 and 12 days in 0.12 and 0.24㎏ a.i./l0a respectively. Range of carbofuran residue in brown rice and rice straw harvested from the paddy agrosystem was 0.01∼0.02 ppm and 0.37∼0.57 ppm irrespective of the two application rates respectively.

토양중 [ 14C ] Carbofuran 의 분해 및 비추출성 잔류분의 특성

이영득(Young Deuk Lee),박창규(Chang Kyu Park) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.3

A study was undertaken to compare degradation patterns of carbofuran in soils between submerged and upland moisture conditions. [3-^(14)C]Carbofuran was treated in each soils at the rate of 1.0 ㎎/㎏ (87.8 kBq ^(14)C/50 g soil) and the time-course analysis for distribution of radioactivity and degradation products were conducted. Differences in the pathway and rate of carbofuran degradation in soils were observed between submerged and upland moisture conditions, major degradation being hydrolysis at 7-C position and oxidation at 3-C position, respectively. Carbofuran showed less persistence in soils of higher moisture contents. A significant portion, 24∼39% of the total radioactivity, resided in soils as nonextractable residues at 60 days after treatment. The nonextractable radioactivity was mainly located in soil organic matter, fulvic acid, humic acid and humin fractions. Gel filtration chromatography confirmed the incorporation of carbofuran and its degradation products into the organic matter.

수상강연 : 학술상 ; 잔류농약 공정 분석법의 확립 및 표준화

이영득 ( Young Deuk Lee ) 한국환경농학회 2009 한국환경농학회 학술대회집 Vol.2009 No.2

HPLC와 LC/MS를 이용한 농산물 중 Strobilurin계 살균제의 잔류분석법

이영득 ( Young Deuk Lee ),김미선 ( Mee Seon Kim ),권찬혁 ( Chan Hyeok Kwon ) 한국환경농학회 2009 한국환경농학회 학술대회집 Vol.2009 No.1