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다래(Actinidia arguta), 양다래(A. deliciosa), 다래×양다래(A. arguta×A.deliciosa) 품종의 엽 형태적 변이 분석
이병실 ( Byoung Sil Lee ),이문호 ( Moon Ho Lee ),황석인 ( Suk In Hwang ),김선창 ( Sun Chang Kim ),한상섭 ( Sang Sup Han ) 한국산림과학회 2003 한국산림과학회지 Vol.92 No.3
Attempts were made to develop varieties having both cold hardiness and high productivity by hybridizing native Actinidia arguta as maternal parent and exotic A. deliciosa as pollen donor. The progenies produced by the hybridization were planted in container within greenhouse, and their morphological leaf characters were examined by discriminant analysis. The results showed that the hybrids resembled their maternal parent in the leaf characters including length of leaf blade, maximum width of leaf, and length of Petiole. However, some characters as the presence of trichomes in leaves, petioles, and stem came from their paternal parent. On the other hand, angle of leaf base, number of serrate, and number of lateral vein appeared to be intermediate of their parents . In the canonical discriminant analysis, primary discriminant function including length of leaf blade, maximum width of leaf, the ratio of length of leaf blade to maximum width of leaf, and the presence of trichomes in leaf, petioles, and stem appeared to be major variables with the contribution proportion of 82.4% in species classification. Although there are extensive variation in leaf characters among the hybrid progenies , many of the characters appeared to be closer to their maternal parent.
은행나무 접목묘의 모수 지역별에 따른 엽 추출물의 flavonol glycosides 및 terpene lactones 함량 특성
황석인 ( Suk In Hwang ),이위영 ( Wi Young Lee ),이문호 ( Moon Ho Lee ),이병실 ( Byoung Sil Lee ),장용석 ( Yong Seok Jang ),이욱 ( Uk Lee ) 한국산림과학회 2004 한국산림과학회지 Vol.93 No.5
Ginkgo tree, Ginkgo biloba L. is a dioecious, coniferous species which is valuable for timber, nuts and high tolerance to air pollution. Also, this species is one of the tree species increasingly explored by medicinal chemists due to its pharmaceutically active leaf terpenes and flavonoids. To clarify the contents of flavonol glycosides and terpene lactones in the leaf, the clonal grafts having the large fruit and high productivity were planted in the same place, and compared with the sites effect of their mother trees. The flavonol glycosides contents showed significant difference among sites of their mother trees; The leaf extracts collected from Jeonnam and Chungnam had higher amounts of flavonol glycosides than those collected from Gyeonggi, China and Chungbuk. However, no significant difference was found in the amount of terpene lactones of extracts among sites of their mother tree. Superior seed bearing trees contented more terpene lactones with significant difference than those of the control trees,but the flavonol glycoside contents didn`t show significant difference between both of them. The contents of terpene lactones in Ginkgo tree leaf were less affected on sites effect than that of flavonol glycosides. In these result, we can suppose that selection of Ginkgo tree getting higher contents of terpene lactones is to get high extraction efficiency for useful substances from the leaf extract.
I-SSR 標識子를 利用한 호도나무屬의 識別 및 類緣關係 分析
黃錫仁,曺炅眞,李文鎬,李在善,李炳實,李郁 한국임학회 2004 한국산림과학회지 Vol.93 No.7
DNA 표지자를 이용한 호도나무 품종의 유연관계 및 품종구분을 위하여 I-SSR 9개 표지를 이용하여 104개의 유의적인 표지가 얻어졌으며, 신품종 호도나무를 포함한 83개 국내외 품종(종)에 대해 DNA 지문에 의한 품종식별이 가능하였다. 수원 1호, 수원 2호, 왕호도는 모두 Conrord, McKinster 등 미국에서 도입된 대립성 품종과의 인공교배로 육성된 품종으로 유전적 유연관계가 상촌 4호 등 국내 선발종보다는 산성 4호×Concord의 교배종과 더 가까웠다. 국내 선발종 호도나무인 상촌 2호, 상촌 4호 및 산성 4호 이들 끼리 한 그룹을 이루고 있는데 이는 국내의 호도나무가 외국품종과 구분되는 유전적 동질성이 있음을 보여주었다. 영동은 개체선발로 육성되었으나 외국종과 교배에 의하여 육성된 교잡종들과 같은 무리에 속하였는데, 이는 영동 품종은 외국 품종의 교배에 의해 외래 유전자 일부가 유입된 것이 아닌가 추정된다. Concord×상촌 1호., Concord×상촌 2호, Concord×대부 1호가 한 무리를 이루었고 Lake×상촌 2호가 이들과 구분되는 것은 교배차대가 유전적으로 화분수보다는 모수의 영향을 더 많이 받고 있음을 보여준다. Using 104 fragments amplified from 9 I-SSR primers, a total of 83 cultivars in Juglans spp. including new cultivars used for the present study were identified. The new cultivars 'Suwon No.1', 'Suwon No.2', and 'Wanghodo' are hybrids between the domestic cultivars and the introduced cultivars such as 'Concord' and 'McKinster' bearing big nuts. Consequently, these cultivars were closer to the hybrid between 'Sansung 4×Concord' than to domestically selected cultivars such as 'Sangehon 4' from the viewpoint of genetic relationship. The domestic cultivars such as 'Sangchon 2', 'Sangchon 4', and 'Sansung 4' belonged to the same group and were genetically distinctive from the introduced cultivars. This suggests that there would be difference in genetic structure between the domestic and the introduced cultivars. The cultivar 'Yeongdong' was clustered into the group of hybrids between the domestic and the introduced cultivars although it was developed by the selection of a tree from a domestic population. This might be because the genes were introduced from the introduced cultivars into the domestic population via open-pollination. The 'Concordx×Sangchon 1', 'Concord×Sangchon 2', and 'Concord×Daebu 1' were Clustered into the same group whereas 'Lake×Sangchon 2' was clustered into the different group. This ndicates that the genetic characteristics of hybrids may be influence more strongly by the mother tree than by the father tree.