유우개량을 위한 유단백질의 유전적 다형에 관한 연구 Ⅰ. αS1 - Casein 및 K - Casein 의 유전적 변이체

한상기(Sang K . Han),이기만(Khy M . Lee),정의용(Eui Y . Chung),장경진(Kyung J . Jang) 한국축산학회 1984 한국축산학회지 Vol.26 No.3

Genetic variants of αs₁-casein (αs₁-Cn) and K-casein (k-Cn) in milk proteins from 138 individual Holstein cattle in Korea were investigated by means of strach-gel-urea electrophoresis and the appearance of phenotypes, gene frequencies and genetic similarities were compared with those of foreign Holstein and of her breeds. The results are summarized as follows: 1. The milk proteins in cattle were controlled by codominant autosomal allelic genes of 9αs₁-Cn^A and αs₁-Cn^B(2 types), and K-Cn^A and K-Cn^B (2 types). 2. The distribution of phenotypes in each milk protein loci was αs₁-Cn BB 124, BC 14; K-Cn AA 76 Ab 49, BB 13. The number of phenotypes coxesponded closely to expectation of Hardy-Weinberg equilibrium (αs₁-Cn:0.7 $gt;P$gt;0.5, k-Cn;0.7$gt;P$gt;0.5). 3. Gene frequencies for genetic variants of the milk protein loci were analyzed. By the predominant allele in αs₁-Cn was αs₁-Cn B with a frequency of 0.949, whereas αs₁-CnC was in low gene frequency (0.051). Among the two alleles for K-Cn, K-Cn A gene showed to occur in high frequency (0.728). However, the Kin B was in low gene frequency (0.272).

돈의 혈정단백질에 관한 연구

이기만,한상기,정의용 ( Khi M . Lee,Sang K . Han,Eui Y . Chung ) 한국축산학회 1984 한국축산학회지 Vol.26 No.4

The phenotypes, genotypes and its gene frequency in genetic variant of Transferrin (Tf) and Pre-albumin (Pa) in serum proteins from total 134 individual pigs, Large White (LW), Duroc (D), Berkshire (B), Landrare (L) and Crossbred (C.B), reared in Korea were investigated by means of starch gel electrophoresis and their gene frequencies and genetic similarities were compared with those of foreign Pig breeds. The results are summarized as follows: 1. Tf and Pa of serum protein in pigs were controlled by co-dominant autosomal allelic gene of TF^A and Tf^B, and Pa^A and Pa^B, respectively. 2. In the phenotypes of Tf of LW, B, L and C.B breeds, Tf AB and Tf BB genetic variants were observed. In the D breed only Tf BB variant was found and the Tf AA homozygous was not observed in all breeds. In the phenotypes of Pa, genetic variants of Pa AA, Pa AB and Pa BB were commonly observed in all breeds. However, one pig of Tf AB heterozygous was appeared in the L breed. It was supposed to crossbred pig because of including A gene. Therefore, it was considered that the purity of a boar and a breed sow in Korea was in need of reexamination. Distribution of the phenotypes of serum protein loci in all the breeds with the exception of C.B breed agrees with Hardy - Weinberg equilibrium. 3. The gene frequencies of Tf B in LW, B, L, D and C.B breeds were 0.714, 0.833, 0.917, 1.000 and 0.160, respectively. In the Pa the LW, D and B breeds showed high gene frequency of the Pa^A variant with 0.571, 0.625 and 0.667, but Pa^B variant showed low gene frequency with 0.429, 0.375 and 0.333, respectively. However, Pa^B with a frequency of 0.583 was slightly higher than that of Pa^A (0.417) in the L breed. Frequencies of 0.571, 0.625 and 0.667 for the LW, D and B breeds showed high gene frequency of the Pa^A, whereas Pa^B showed low gene frequency (0.429, 0.375 and 0.333, respectively). The Pa^B with 0.604 gene frequency in C.B breed was also high, but Pa^A was in low gene frequency of 0.396.

한우의 Albumin ( Alb ) 형 및 Post - albumin ( Pa ) 형에 관한 연구

한상기,이기만 ( S . K . Han,K . M . Lee ) 한국축산학회 1982 한국축산학회지 Vol.24 No.6

Albumin and post albumin types in korean cattle were investigated by means of starch gel electrophoresis. 1) The distribution of Alb phenotypes in 108 korean cattle was Alb-AA type 105 and Alb-AB type 3. The Alb BB type has not yet been reconiged in korean cattle. The gene frequencies were Alb^A=0.986 and AIb^B=0.014. The gene Alb^C and Alb^B, which have not been reconiged in european cattle, were found at relatively high frequencies in formosan yellow cattle, at low frequencies in korean cattle, and very rare in a Japanese breed. According to these results it is supposed that Japanese native cattle originated from korean cattle, and these two breeds might have been somehow influenced by yellow cattle. 2) The distribution of Pa phenotypes in 107 korean cattle was Pa-AA type 9, Pa-AB type 49 and Pa-BB type 49. The gene frequencies were Pa^A=0.313 and Pa^B=0.687.

한우 및 Holstein 의 Hemoglobin 형에 관한 연구

한상기,이기만 ( S . K . Han,K . M . Lee ) 한국축산학회 1982 한국축산학회지 Vol.24 No.6

Hemoglobin samples of 108 Korean cattle and 60 holstein were studied by starch gel electrophoresis. Genetic polymorphism of hemoglobins was established in korean cattle and holstein. 1) Hemoglobin types from 108 korean cattle and 60 holstein were as follows: 86 of Hb-AA, 18 of Hb-AB, 3 of Hb-AC, 1 of Hb-BB in korean cattle and 60 of Hb-AA in holstein. 2) These figures correspond to the frequencies: Korean cattle was Hb^A=0.895, Hb^B=0.071, Hb^C=0.014 and holstein was Hb^A=1.000. Phenotypes Hb-AA and Hb-AB had the highest frequencies in korean cattle, whereas Hb-BBand Hb-Ac occur very rarely and the homozygote HbCC and Hb-BC were not recognized.

유우개량을 위한 유단백질의 유전적 다형에 관한 연구 Ⅱ. β- Casein 및 β- Lactoglobulin 의 유전적 변이체

한상기(Sang K . Han),이기만(Khi M . Lee),정의용(Eui Y . Chung),장경진(Kyung J . Jang) 한국축산학회 1984 한국축산학회지 Vol.26 No.3

Genetic variants of the two milk proteins, β-casein (β-Cn) and β-lactoglobulin(β-Lg), from 138 individual Holstein cattle reared in Korea were investigated by means of starch-gel-urea electrophoresis and their phenotypes, genotypes, gene frequencies, linkage of gene and genetic similarities were compared with those of foreign Holstein and other breeds. The results are summarized as follows: 1. The milk proteins were genetically polymorphic in cattle and were controlled by codominant autosomal allelic genes of βCn A1, βCn A2, β-Cn A3, and βCn B(4 types), and (β-Lg A and β-Lg B(2 types). 2. The phenotypes showed the following distribution of the two milk protein loci: β-Cn A1 A1 34, A1A2 55, A2A2 30, A1A3 4, A2A3 7, A1B 3, A2B 5; βLg AA 25, AB 73, BB 40. The numberofphenotypescorresponded closely to expectations of Hardy-Weinberg equilibrium (β-Cn: 0.9 $gt;P $gt; 0.8, β-Lg: 0.7 $gt; p$gt;0.5). 3. Gene frequencies for genetic variants of the milk protein loci were determined by counting identified alleles. In the β-Cn loci, the β-Cn A1 and A2 variants were predominant with a frequency of 0.471, 0.460, respectively. However, the β-Cn A3 and β-Cn B genes were rare (0.040 and 0.029, respectively). Gene frequencies of β-Lg were 0.446 and 0.554 for alleles A and B, so frequency of the β-Lg^B variant was slightly higher than that of β-Lg A variant. 4. Genetic linkage of the casein loci was αs₁-Cn^B/β-Cn^A 116, αs₁-Cn^(BC)/β-Cn^A 14, and αs₁-Cn^(BC)/β-Cn^(AB) 8. The most close linkage of gene between the αs₁-Cn^B and β-Cn^A has been observed. 5. These results indicate that gene frequencies of the milk protein loci of Holstein cattle in Korea are fairly similar to those of foreign Holstein, but are markedly different from those of other European dairy breeds and Korean native cattle, especially Hariana breeds of Zebu.

닭의 경제형질에 대한 유전력 및 유전상관에 관한 연구

정선부,설동섭,탁태구,김상철,이기만 ( S . B . Chung,D . S . Sul,T . Y . Tak,S . C . Kim,K . M . Lee ) 한국축산학회 1973 한국축산학회지 Vol.15 No.3

The purpose of this study was to evaluate the value of initial egg weights as selection criterion of laying hens for improvement of egg production performance. Data here used came from records of 406 white leghorn hen progenies produced by hierachal mating of 44 sires and 201 dams at three different hatching stages, which were maintained at Livestock Experiment Station. The data were analysed by the method of variance component analysis to estimate heritabilities, maternal variance, on 5 economic traits and genetic, phenotypic and environmental correlations among them. The results obtained were as follows: 1. The heritability estimates were 0.549 for body weight at first egg, 0.500 for age at first egg, 0.160 for initial egg weight, 0.398 for matured egg weight and 0.429 for egg number to 500 days. 2. The fraction of the total variance which were due to the maternal effect was 0.175 for body weight at first egg, 0.204 for age at first egg, -0.058 for initial egg weight, 0.140 for matured egg weight and 0.095 for egg number to 500 days. 3. The genetic correlations of body weight at first egg with age at first egg, initial egg weight, matured egg weight and egg number to 500 days were -0.503. 0.300, -0.186 and -0.105, respectively. The genetic correlations of age at first egg with initial egg weight, matured egg weight and egg number to 500 days were 0.501, -0.238 and -1.969. The genetic correlations of initial egg weight with matured egg weight and egg number to 500 days were 0.493 and -0.537. There was 0.068 of genetic correlations between matured egg weight arid egg number to 500 days. On the basis of the results obtained in this study, it is recommented that for the effective improvement of egg weight, selection should be practiced for initial egg weight.

Saanen 종과의 누진교잡에 의한 재래산양 개량시험 제2보 , Saanen 종과 재래산양의 교잡종에 대한 비유능력의 변화

이길왕,최광수,탁태영,설동섭,이기만 ( K . W . Lee,K . S . Choi,T . Y . Tak,D . S . Sul,K . M . Lee ) 한국축산학회 1975 한국축산학회지 Vol.17 No.1

The data from 393 purebred and crossbred goats produced by matings of Korean Native Goats and Saanen, which were investigated at Livestock Experiment Station since 1963, were analyzed by least-square method to investigate changes of milking performance of the Korean Native Goats by grading up with the Saanen. This procedure adjusted the measurement data for surveyed year, parity, mating system and for the number of kid born at one birth. The results obtained are as follows. 1. The milk productions of the Korean Native Goats, the first filial, the second, and the third graded generations were 423.91, 90.97, 288.12, 354.81 and 372.76㎏, respectively. Their milk fat percentages were 4.15, 5.72, 5.37, 4.30 and 4.24, respectively. Their lactation periods were 239.7, 173.2, 214.3, 236.2 and 234.2 days, respectively. 2. The milk performances of the first filial, the second, the third graded generation and Saanen were significantly higher than those of Native Goats. The increment of milk character is the filial, the second, and the third graded generation, compared to the Native Goats were 197.15, 263.94 and 281.79㎏ in milk production; 41.14, 63.05 and 61.05 days in lactation period; -0.34, -1.42 and -1.48% in milk fat percentage. 3. The difference of milk production, lactation period and milk fat percentage between the third and the second graded generation was 17.89㎏, -2.00 days and -0.06, respectively. The difference between purebred Saanen and the third graded generation was 51.15㎏ for milk production, 1.55 days for lactation period, and -0.08% for milk fat percentage. However there was no significant difference between them. 4. The individual comparisons among the least square estimates showed that the difference between the 3 and 1 kid per birth was 130.4㎏ for milk production, 29. 4 days for lactation period, and -0.7% for milk fat percentage. The difference between the 3 .and 2 kid per birth was 3.13㎏, 11.6 days and 0.02%, respectively. The difference between the 2 and 1 kid per birth was 41.6㎏, 17.8 days and 0.09%, respectively.

로봇을 이용한 패딩용접 좌표 연계 기법 연구

이은하(E. H. Lee),이기만(K. M. Lee) 한국생산제조학회 2022 한국생산제조시스템학회 학술발표대회 논문집 Vol.2022 No.12

Saanen 과의 누진교잡에 의한 재래산양 개량 시험 제1보 Saanen 과 재래산양의 교잡종에 대한 월령별 체중변화

이길왕,최광수,설동섭,이기만 ( K . W . Lee,K . S . Choi,D . S . Sul,K . M . Lee ) 한국축산학회 1973 한국축산학회지 Vol.15 No.4

The data from 112 purebred and crossbred goats produced by matings of Korean native goats and Saanen, which were investigated at Livestock Experiment Station since 1963, were analysed by least square method to investigate changes of body weights of the native goats by grading up with Saanen. This procedure adjusted the measurement data for environmental effects of year of birth, age of dam and litter size. The results obtained were as follows; 1. The body weight was measured and adjusted at birth, weaning, 12 and 18 months of age as 1.83㎏, 6.99㎏, 14.51㎏ and 18.90㎏ in Korean native goats, 2.98㎏. 12.25㎏, 25.49㎏ and 36.93㎏ in purebred Sannen, 2.69㎏, 11.14㎏, 23.03㎏ and 29.27㎏ in the first filial generation, and 2.78㎏, 11.85㎏, 24.77㎏ and 36.19㎏ in the second graded generation, respectively. 2. The body weights of first filial and second generation were heavier than those of native goats through all months of age. The increment of body weight in the first filial generation to the native goats was 0.86㎏ at birth, 4.15㎏ at weaning, 8.50㎏ at 12 months and 10.37㎏ at 18 months of age. In the second generation the increment of body weight was 0.94㎏, 4.85㎏, 10.26㎏ and 17.29㎏, respectively. 3. The differences of body weight between purebred Saanen and the first generation were 0.28㎏ at birth, 1.11㎏ at weaning 2.4㎏ at 12 months and 7.6㎏ at 18 months of age, but significant difference was found only at 18 months of age (p$lt;0.01). The body weight of the second generation was slightly lighter by 0.20 to 0.75㎏ than that of purebred Saanen through all months of age. 4. The second generation was heavier than the first generation by 0.08㎏ at birth. 0.71㎏ at weaning, 1.73㎏ at 12 months and 6.92㎏ at 18 months of age, but significant difference was found after 18 months of age (p$lt;0.05).

디젤연료 분무연소시 수증기 첨가에 따른 NOx 저감에 관한 연구

채재우(J . O . Chae),이상만(S . M . Lee),정영남(S . C . Chung),정성찬(Y . N . Chun),정영식(Y . S . Jeong),이기만(K . M . Lee) 大韓環境工學會 1993 대한환경공학회지 Vol.15 No.6