동결 절편에 있어서 Dimethyl Sulfoxide가 미치는 영향

윤인숙 대구보건대학 1992 대구보건대학 論文集 Vol.13 No.-

On the basis of the fact which Dimethyl sulfoxide(DMSO) penetrate tissue rapidly and reduce the size of ice crystals, a test was done to know whether DMSO can be used in the process of frozen sections. Small blocks of liver, Kidney, lung and spleen tissues were dissected form rats and were divided each into four portions. One block of each tissue was placed for 20 to 25 minutes in each of the following solutions : normal saline(NS), 5%(V/V) DMSO in NS, 10% DMSO in NS, and 20% DMSO in NS. Frozen sections were done on these tissues, and then hematoxylin-eosin stain was done and compared. Generally there was less tissue disruption in the samples treated with DMSO than without DMSO. Especially, in all the tissues, the 10% DMSO gave the best results in staining, cutting and the thickness of the tissue sections. Tissue treated with 20% DMSO was very hard to cut and could not be cut as thinly as the 5% or 10% DMSO treated tissue. Especially in the liver tissue, shrinkage of tissue sections was occured. The kidney tissues treated with 5% DMSO and 10% DMSO were comparable in reducing tissue disruption. The DMSO does not interfere with formalin fixation after frozen sections in the process of routine preparation. The time of penetration of the DMSO into the tissue block was very fast and penetration was apparently completed by ten minutes. If the time of treatment with DMSO was longer, it stained darkerly with hematoxylin-eosin. All of these findings suggest that treatment with 10%(V/V) DMSO for frozen sections enhances the quality of the frozen sections. If possible, this method should be used for human pathologic specimens through the examination on applying DMSO to human tissues.

The Effect on the Liver Function in Rats Treated with Xylene and Ethanol

윤인숙 ( In Sook Yoon ) 대한임상병리사협회 2005 조직세포검사학회 발표자료집 Vol.2005 No.-

Background : Xylene is an aromatic hydrocarbon that has a ring. It is included in various materials such as paint, plastic, rubber paste, aircraft fuel, diluent, printing solvent. However, xylene is relevant to medical technologists because of its use in making slides for biopsy in hospital laboratories. Xylene is mainly metabolized in the liver via the oxidative pathway. There are reports that xylene can bring about damage to the liver, nervous system, and hematogenesis when it is exposed in humans. Ethanol is human favorite food but it is the most frequently ingested toxin throughout the world. Nevertheless, the high amount of alcoholic consumption said to cause the improvement of the living standard, which allows us more leisure time as well as reducing occupational stress. So, the effects of alcohol on liver function were studied, the group being that of laborers who handle xylene. Methods : Normal male rats of the Sprague-Dawley strain, weighing between 320g and 350 g, were used in this experiment. All the rats were fed on a standard rat chow as well as water. Experiments were performed in four groups; A Control Group, an Ethanol Group, a Xylene Group, and an Ethanol + Xylene Group. Urine specimens were collected in metabolic cage for 24 hours on the last day of receiving xylene injection. Blood samples were obtained from the abdominal aorta under light ether anaesthesia. AST, ALT, LDH and ALP levels in the serum were measured to estimate the levels of liver function. MDA, which gives an indirect index of oxidative injury was also measured. MMHA which is the last metabolic production of xylene was measured with HPLC. Liver tissues were resected and fixed in 10% formalin, stained with H&E. Results : The biochemical results suggest that the Ethanol Group had significantly higher levels of AST, ALT and LDH than those of the Control Group. Also, the Xylene Group had notably high levels of AST, ALT, LDH as well as high levels of MMHA than those of the Control Group. The levels in the Ethanol + Xylene Group was drastically higher than both the Control and Ethanol Groups. Also, the said levels in the Ethanol + Xylene Group were significantly higher than those of the Xylene Group. But, there were significantly lower ALP levels in the Xylene Group and Ethanol + Xylene Group than both the Control and Ethanol Groups. The Ethanol + Xylene Group had significantly higher levels of AST, ALT and LDH than those of the Xylene Group. The histological features of the rat livers treated with alcohol, or xylene showed normal. But the rat livers treated with alcohol + xylene showed mild to moderate necrosis and inflammation, and minimal fatty changes. Conclusions : To conclude the experiment on liver function of rats with ethanol intoxication combined with xylene hepatotoxicity, one can generally state that the Ethanol + Xylene Group had worsened liver function, more so than that of the Xylene Group. Accordingly, the results in this experiment suggest that liver function is far worse when medicated together with xylene and ethanol rather than medicated xylene according to the rat experiment model. Therefore, laborers who handle xylene should seriously consider health aspects before consuming alcohol, or at least control their often heavy drinking habits. HTs must be protected from harmful environments which expose them to xylene and ethanol. The histology room must be standardized with modern ventilation equipment at the standpoint of the Ministry of Environment or the Ministry of Health and Welfare.

혈액형에 따른 여가활동 유형 -대구보건대학 보건계열 및 비보건계열 학생들을 중심으로-

윤인숙 ( In Sook Yoon ),김혜정 ( Hye Jong Kim ) 대한임상검사과학회 2006 대한임상검사과학회지(KJCLS) Vol.38 No.1

We examined the relationship between the basic characteristics and types of leisure activities according to blood types. It was based on questionnaires answered by 441 collegians in public health departments and 282 in non-public health departments in Daegu Health College in March, 2005. The frequency of leisure activity of once or twice a week or less during weekdays was the most common.: The most common frequencies for all blood types were between 1 and 2 hours on weekdays and between 3 and 5 hours on the weekends or holidays. As far as the type of leisure activities during the weekdays was concerned, in the case of public health departments, blood type A was interested in computer games and then watching TV, blood type B was attracted to watching TV and listening to music, blood type AB was into watching TV, computer games, and listening to music, and blood type O was keen on watching TV and computer games in that order. The type of leisure activity according to blood types in public health departments was shown to be significantly different (p<0.05). The type of future leisure activity was shown to be significantly different between public health departments and non-public health departments (p<0.05). The type of present leisure activity preferred by collegians, according to the study, was entertainment such as computer games for most blood types. The type of future leisure activities was related to the field of tourism and sports. The collegians need to have more advanced and positive leisure activities.

중국 연변 조선족 여성의 자궁경부세포 검사소견

윤인숙 ( In Sook Yoon ),이혜자 ( Hye Ja Lee ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

According to literature, the risk of developing cervical invasive carcinoma is at least 10 times greater in women not screened by a cervical Papanicolaou smear than in women screened by the same test. We performed cervical Pap smears to investigate the causes and rate of cervical cancer in Yanbian Korean-Chinese women who could not receive the Pap test until now. We administered a questionnaire concerning epidemiological factors of cervical cancer and a free cervical Pap smear was given. It was administered three times from April 2000 to July 2001 for the purpose of producing basic data concerning the management of cervical cancer in Yanbian Korean-Chinese women. Women who had previously received Pap smear tests comprised of 3.6% or 25 out of every 704 women in Yanbian. The results of the Pap smears in the 704 women showed two squamous cell carcinomas(0.3%), 14 LSILs(2.0%), 20 ASCUSs(2.8%), 390 inflammatory changes(55.4%) and 278 negatives(39.5%). Of special note, the HPV infection rate of the Yanbian Korean-Chinese women was recorded as 1.7%(12 persons) which is higher than any other rate found in the literature. I will therefore continue the investigation of cervical HPV typing.

흰쥐의 톨루엔 간독성에 었어서 Vitamin C 와 E의 효과

배시우 ( Si Woo Bae ),윤인숙 ( In Sook Yoon ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2

툴루엔은 주로 간에서 산화적 경로에 의해서 대사 된다. 활성산소는 톨루엔 대사과정에서 발생된다. 그러므로 툴루엔 대사과정에서 발생되는 활성산소에 의해 조직 및 세포상해를 야기 시킬 수 있다. Vitamin C와 E는 항산화제로 활성산소에 의한 손상으로부터 세표플 보호하는 데 고효율로 활성산소를 제거하는 역할을 한다. 툴루엔의 간 독성에 대한 가섣판검정하기 위해 툴루엔을 쥐의 복강에 주사하였다. 그라고 vitamin C와 E가 쥐간을 보호하는지를 알아보기 위해 쥐에게 톨루엔 주사와 함께 vitamin C와 E룰 경구 투여하였다. 간기능검사로 혈청 AST, ALT, LDH, ALP를 측정하였다. 산화적 손상의 간접지표인 MDA도 측정하였다. 툴루엔의 최종대사산물인 마뇨산은 매LC로 정량하였다. AST, ALT, LDH, MDA와 마뇨산은 툴루엔군이 대조군에 비해서 현저하게 높았다. 그러나 vitamin C와 E군에서는 ALT와 마뇨산을 제외하고는 현저한 차이는 없었다. ALP는 툴루엔군에서 현저하게 낮았으나 vitaminC와 E군에서는 현저한 차이는 없었다. 이상의 결과로 보아 툴루엔이 대사 될 때 활성산소에 의해 간 독성이 야기되었음을 알 수 있다. 그리고 vitamin C와 E를 투여했을 때 툴루엔으로 인한 간의 손상을 방어해 줌을 알 수 있다. 따라서 이 연구는 vitamin C 와 E 가 툴루엔에 의한 급성 간 중독시 간 손상을 방어하는 데 도움이 된다고 사료된다. Toluene is mainly metabolized in the liver by the oxidative pathway. Oxygen free radicals occur through the process of toluene metabolism. Therefore, tissue and cell injury occurs by oxygen free radicals from the metabolism of toluene. Vitamin C is a water-soluble antioxidant that maintains many metal cofactors in the reduced state. Vitamin E( -tocopherol) acts as an antioxidant, breaking free-radical chain reactions as a result of their ability to transfer a phenolic hydrogen to a peroxyl free radical of a peroxidasised polyunsaturated fatty acid. Therefore vitamin C and E act as highly efficient free radical scavengers that protect cells from damage by oxygen free radicals. To test this hypothesis, toluene hepatotoxicity was induced by an abdominal injection of toluene. To see if vitamin C and E protects the rat``s liver, vitamin C and E was administrated orally, at the time of each toluene injection. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), latic dehydrogenase (LDH) and alkaline phosphatase (ALP) levels in serum were measured to estimate hepatic function. Melondialdehyde (MDA), which gives an indirect index of oxidative injury was also measured. Hippuric acid, the last metabolic production of toluene, was measured by HPLC. There were significantly higher values in AST, ALT, LDH, MDA and hippuric acid in the toluene group, but the values were mostly lower in vitamin C and E groups than in toluene group. There were significantly lower values in ALP in the toluene group, but there were no significant differences between the vitamin C and E groups, suggesting a significant hepatotoxicity due to oxygen free radicals through the process of toluene metabolism. Vitamin C and E treatment significantly protected hepatic function and free radical-mediated injury in the liver against toluene-induced changes. Accordingly, this study shows that vitamin C and E is helpful in protecting against liver injury by acute toluene intoxication.

흰쥐의 Toluene 간독성에 있어서 Taurine과 Ginseng의 효과

배시우 ( Si Woo Bae ),윤인숙 ( In Sook Yoon ) 대한임상검사과학회 2003 대한임상검사과학회지(KJCLS) Vol.35 No.2

Toluene is mainly metabolized in liver by oxidative pathway. Oxygen free radicals occur through the process of toluene metabolism. Therefore it causes tissue and cell injury by the oxygen free radicals from the metabolism of toluene. Taurine is a nonessential amino acid that is of medical interest for the nutrition of infants. Taurine is known as an antioxidant that prevents organ damage by scavenging reactive oxygen species. Ginseng is one of the most popular herbal remedies and ginseng has been used as a traditional medicine with various therapeutic effects. And there are interesting report that ginseng has antioxidant reaction. To test this hypothesis, toluene hepatotoxicity was induced by an abdominal injection of toluene. To see if taurine and ginseng protect the rat`s liver, taurine and ginseng was administrated orally, at the time of each toluene injection. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), latic dehydrogenase (LDH) and alkaline phosphatase (ALP) levels in serum were measured to estimate hepatic function. Malondialdehyde (MDA), which gives an indirect index of oxidative injury was also measured. Hippuric acid is the last metabolic production of toluene was measured by HPLC. AST, ALT, LDH and MDA levels were significantly higher in toluene group than in control group, but there were no significant differences between taurine and ginseng groups and control group. However LDH and MDA levels were significantly higher in taurine and ginseng groups than in control group. ALP levels were significantly lower in toluene and taurine groups than in control group. Hippuric acid levels were significantly higher in toluene, taurine and ginseng groups than in control group. But there were significantly lower in taurine and ginseng groups than in toluene group. Accordingly, the results suggest a significant hepatotoxicity due to oxygen free radicals through the process of toluene metabolism. Taurine and red ginseng extract treatment significantly protected hepatic function and free radical-mediated injury in the rat`s liver against toluene-induced changes. Therefore, the results in this experiment suggest that taurine and red ginseng extract are effective in preventing the toluene-induced hepatotoxicity in a rat model.

알레르기 환자에서 Immunoglobulin E와 Eosinophil count 및 Allergen과의 관련성

윤인숙(In-Sook Yoon),권세영(Se-Young Kwon) 한국콘텐츠학회 2009 한국콘텐츠학회논문지 Vol.9 No.4

알레르기 환자의 증가로 인하여 알러겐을 찾는 것은 중요하다. MAST는 알러겐 특이 IgE 항체를 측정하는 간단한 방법이다. 본 연구에서는 알레르기 환자에서 알러겐 특이 IgE 항체, 총 IgE, 호산구수와의 관련성을 평가하고자 하였다. 연구 결과 총 IgE 양성률은 흡입형 패널에서 96.97%, 식품형 패널에서 98.06% 였다. 알러겐 특이 IgE 항체의 양성률은 흡입형 패널에서 집먼지 51.52%, 북아메리카 집먼지 진드기 45.46%, 고양이 31.99% 였으며, 식품형 패널에서 각각 55.34%, 42.72%, 34.96%였다. 혈청 총 IgE는 알레르기와 관련이 있었으나, 알레르기가 항상 호산구증가와 관련이 있는 것은 아니었다. As the allergic diseases in patients are increasing, it is important to find out the allergens. A multiple antigen simultaneous test(MAST) is a simple method for in vitro measurement of allergen-specific IgE antibodies. This study was performed to evaluate the relationship between allergen-specific IgE antibodies, serum total IgE and peripheral eosinophil count in the allergic patients. According to the results of the study, the total IgE positive rate(above class 2) from the inhalent is 96.97%, and that from food panel is 98.06%. The research showed that the positive rate of the allergen-specific IgE was House dust 51.52%, D. farinae 45.46%, Cat 31.99% in inhalent panel, and 55.34%, 42.72%, 34.96% in food panel. Serum total IgE was associated with allergy, however, allergy was not always associated with eosinophilia.

흰쥐에게 Ethanol과 Xylene 병행 투여시 간손상에 미치는 영향

윤인숙(In-Sook Yoon),이혜자(Hye-Ja Lee) 한국콘텐츠학회 2010 한국콘텐츠학회논문지 Vol.10 No.7

흰쥐에게 ethanol과 xylene을 투여시 간손상 정도를 알아보기 위해 정상적인 S-D계 수컷 흰쥐를 4군으로 나누어 실험을 수행하였다. 생화학적 검사 결과에서 혈청 AST, ALT, LDH활성은 대조군보다 ethanol 투여군, xylene투여군, ethanol+xylene투여군으로 갈수록 활성이 유의하게 증가되었으며, 그 증가율은 ethanol+xylene투여군에서 가장 높게 나타났다. 산화적 손상정도를 알아보기 위한 혈청 MDA함량은 대조군보다 ethanol투여군과 xylene투여군 그리고 ethanol+xylene투여군으로 갈수록 활성이 증가되었으며, 그 증가율은 ethanol+xylene투여군에서 가장 높게 나타났다. 그러나 혈청 ALP는 대조군에 비해 모두 감소하였다. Xylene의 투여시 최종대사산물인 요 중 m-methylhippuric acid는 대조군에 비해 xylene투여군과 ethanol+xylene투여군에서 증가되었으며, 그 증가율은 xylene투여군이 ethanol+xylene투여군보다 더 높게 나타났다. 간 조직검사에서는 xylene투여군은 지방변화를, ethanol+xylene 투여군은 경도~중등도의 괴사와 염증을 보였다. 이상의 혈청학적 검사와 조직학적 검사결과 xylene 단독 투여에 비하여 ethanol과 xylene을 병행 투여했을 때 간손상이 더 크게 초래된 것으로 나타났다. To evaluate an effect of liver damage on ethanol and xylene exposure, experiments on normal male rats of the S-D strain were performed in 4 groups. The biochemical results suggest that the ethanol group had significantly higher levels of AST, ALT, LDH and also, the xylene group had notably higher levels of AST, ALT, LDH along with MMHPA than those of the control groups. The levels of AST, ALT and LDH in the ethanol+xylene group were drastically higher than those in the control, ethanol and xylene groups. But, there were significantly lower ALP levels in the xylene and ethanol+xylene groups than both the control and ethanol groups. The histological features of rat livers treated with alcohol, or xylene proved to be normal. But the rat livers treated with ethanol+xylene showed mild to moderate necrosis and inflammation as well as minimal fatty changes. The results in this experiment suggest that liver functions decreased when medicated together with xylene and ethanol rather than solely with xylene.

증례(症例) : Pemphigus Vulgaris의 세포학적, 조직학적 그리고 임상적 상호관계

윤인숙 ( In Sook Yoon ) 대한임상검사과학회 1983 대한임상검사과학회지(KJCLS) Vol.15 No.1

A case of pemphigus vulgaris is reported with a review of previous literatures. The only case selected for this study was that in which the clinical, histologic and cytologic diagnoses were in agreement. The patient showed an oral manifestation, which appeared 9 months ahead of the cutaneous lesions. In Mar. 1980 the patient became aware of a few intraoral lesions. She went to the pharmacy and was give medication. But her intraoral lesions continued to spread. From several weeks prior to admission, she developed some blisters -around her external genitalia. A few days later numerous bullous lesions appeared on her back, axilla, scalp, neck, extremities and in the midface around her eyes. She was admitted to the hospital on the 28th. of Dec. 1980, at ,which time a skin biopsy and smears were taken from the erupted bullae on her left thigh. The biopsy was interpreted as consistent with the tissue of pemphigus vulgaris and the smears showed cellular changes which are compatible with pemphigus. I want to clarify here that the clinical information was derived from the archives of the recorded patients` documents.

대구지역 초등학생의 흡연과 음주실태 및 그 관련요인

김경희 ( Kyong Hee Kim ),윤인숙 ( In Sook Yoon ),서수교 ( Soo Kyo Seo ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2

This research paper analyzed the smoking and drinking rea1ity of primary school students, through the survey of 10 primary schools`` students randomly chosen in Taegu City 1217 students the‘ upper the grades 4, 5, 6 with 688 boys and 529 girls. Among these students, 9.6%experienced smoking 15.0% for boys and 2.6% for girls. In general, throughout the survey, it was discovered that there was a high significance of smoking for those boys who were from unstable and lower social-economic classes or living away from the family (p<0.01). The parents of the students influenced their children as to whether students began smoking or not; if they did not care about their children’s smoking or if fathers and mothers were not getting along with each other, the children were involved in smoking, and the significance was high (p<0.01). Among these students, 44.8% experienced drinking 367 boys (53.3%) and 178 girls(33.6%). In general, it turned out that there was a high significance of drinking for those boys who were unstable classes or the unsatisfactory groups (p<O.Ol). πle parents of the students inf1uenced drinking of the students; if they didn’t care about their children’s drinking, or if they themselves enjoyed dri따ing, the significance of drinking was high (p<0.05, p<O.Ol). Also, the first experience of smoking or drinking was relatively higher in the groups of grades 3 and 4 as well as the next higher in groups of 1 and 2, but it was the lowest in groups of grades 5 and 6. Homes were the most highly picked places for smoking or drinking. The strongest motivation was curiosity as well as the experienced students did one or two times of smoking or drinking a week. In relation to school life, the poorer the sch。이 work, the unhappier the school life, the more students were involved in smoking or drinking, me따ung the significance was high (p<0.05, p<O.Ol). However, if the students’ friends were not involved in smoking and drinking, and if the punishment was not announced and not practiced, the significance was also high (p<0.01) Throughout the result of analysis, for the reality of primary school students`` smoking and drink.ing, the family environment, the environment of parents, and school life, the knowledge on smoking and drinking related with the hea1th were significant on smoking and drink.ing of the students. Therefore, to prevent students from smoking and drinking, schools, family, and society should work organically together and tried to guide students to the desirable directions.