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      • KCI등재후보

        Simultaneous analysis and monitoring of thirteen preservatives in cosmetics by liquid chromatography

        류영재,윤영원,이범준,윤건묵,윤방한,이보영,민필기,윤지영,남상윤 한국예방수의학회 2013 예방수의학회지 Vol.37 No.2

        The content of 13 preservatives was investigated for 107 sold in retail markets. A simultaneously analytical method of liquid chromatography (LC) for the preservatives in cosmetics was developed using an AcQuityTM Ultra Performance LC System (Waters), BEH C18 column (150 mm✕2.1 mm, 1.7 ㎛) and Photo Diode Array (PDA) detector (λ; 220 ㎚ for quantitative analysis, λ; 190~400 ㎚ for qualitative analysis). The developed instrumental method was validated using limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, and linearity of standard curves. LOD and LOQ were lower than 0.23 ㎎/ℓ and 0.75 ㎎/ℓ, respectively. When standard compounds were analyzed seven times consecutively at the LOQ level, precision and accuracy were lower than 2.49% and 2.04%, respectively. Good linear relationships (R2≥0.9998) were obtained for all compounds. Recoveries ranged between 96.03% and 102.61% and the overall variability was below 3% (RSD). The type of cosmetics showing the lowest detection rate of target compounds was sunscreen lotion, with a detection rate of 42.9%, followed by baby lotions and mask packs with the same detection rate at 69.2%. At least one of the target preservatives was detected in 82.4% of creams, 93.3% of skin toners, 95.2% of lotions, and 92.9% of body lotions. Overall, 79.4% of all samples contained at least one of the target compounds. The most frequently detected compound was phenoxyethanol. Methyl and propyl paraben also had a higher detection rate than the others, except phenoxyethanol. Although the target preservatives in cosmetics sold in retail markets were detected by the simultaneous analysis of LC, the content of the detected preservatives was within the maximum allowed amount established by the Korean FDA.

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