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유용구,이희종,김효준 한국바이오칩학회 2009 BioChip Journal Vol.3 No.2
Human mitochondrial adenylate kinase plays an important role in energy metabolism by phosphoryl transfer between adenine nucleotides. Our previous studies had strongly suggested that the serum levels of human adenylate kinase 3 (hAK3) should be a biochemical marker for the necrosis of myocytes. Also, it was reported that the expression of AK3 was induced about 3 fold in the condition of hypoxia. Acute myocardial infarction (AMI) is a disease appeared when cardiac muscle cell disrupted by hypoxia. Monoclonal antibodies (Mabs) are produced widely for use in researches and also are produced in large quantities against a plethora of antigens for use in diagnosis and sometimes in treatment. In this study, B lymphocyte hybridoma cell lines producing MAb against hAK3 were constructed and characterized. For the proper and optimal applications, antibodies were grouped and characterized according to their physicochemical properties and antigenic structural characters. Identification and compairson for the complementarity determining regions (CDRs), the avidities in terms of dissociation constants and isotypes were made for each Mabs. This Mabs are useful in protein diagnostic lab-on-a-chip technology.
Local Ordering Study of Nanostructured FeCo-C Alloys for Various Fe Contents
유용구,Bingzhi Jiang,양동석,G.M. Greneche,유성초 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.48 No.6
Nanostructured (FexCo1¡x)75C25 alloys were prepared by mechanical alloying. Local ordering around the central atom was examined for various Fe contents by using extended X-ray absorption .ne structure. X-ray diraction patterns indicate a cohenite-like phase for composition of x = 0.2, 0.5, and 0.8. The .rst shell of the Fourier transformed spectra changed with Fe content, indicating a change in the local ordering around the central Fe or Co atom. The x = 0.5 composition was well .tted to a (FeCo)3C phase, and the interatomic distance between the central atom and neighboring atoms was determined. The temperature dependence of magnetization was also studied for various Fe contents.0