PCS OTASP를 위한 OTAF 시스템 설계 및 구현

강경진(Kyoung-Jin Kang),유영대(Young-Dae Yu),정태식(Tae-Sik Jung),김윤경(Yun-Kyung Kim) 대한전자공학회 1998 대한전자공학회 학술대회 Vol.1998 No.4

불안정 지지면에서의 유산소 운동이 심폐기능과 균형능력에 미치는 영향

권효륜(Hyo-Ryun Kwon),김미소(Mi-So Kim),남유정(Yu-Jung Nam),박혜민(Hye-Min Park),이경선(Kyeong-Sun Lee),조홍덕(Hong-Deok Jo),최동원(Dong-Won Choi),양회송(Heo-Song Yang),유영대(Young-Dae Yoo),강효정(Hyo-jeong Kang),정찬주(Chan-Joo Jeo 대한심장호흡물리치료학회 2018 대한심장호흡물리치료학회지 Vol.6 No.1

The purpose of this study was to investigate the effects of aerobic exercise with unstable supporting surface on cardio-pulmonary function and balance ability. Methods : This study was performed on 20 subjects. The subjects were divided into two groups; stable supporting surface group(Group Ⅰ, n=10), unstable supporting surface group(Group Ⅱ, n=10). The groups performed the aerobic exercise, which is jump rope exercise, 2 times a week for 4 weeks. Results : The results were as follows. There was statistically significant difference of cardiovascular endurance in Group Ⅰ(p<0.05), and there were statistically significant difference of cardiovascular endurancedynamic, OSI and dynamic APSI in Group Ⅱ (p<0.05). However, there were no statistically significant difference in pulmonary functions which were FVC and FEV1 in Group Ⅰand Group Ⅱ. Conclusion : As a result of this study, aerobic exercise with unstable supporting surface have a potential to induce positive responses in cardiovascular endurance and dynamic balance ability of healthy adults.

세균액 및 세균단백질 추출물이 배양 세포에 미치는 영향

유영대,임미경 원광대학교 치의학연구소 2000 圓光齒醫學 Vol.10 No.1

Dental pulp infection is most commonly caused by extensive dental caries, and some bacterial species invade root canals; bacterial components and products are thought to be associated with the pathogenesis of periapical periodontitis. A principle driving force behind pulpal disease response appears to lie in the host immune system's to bacteria and their products. We examined the production of interleukin 1β (IL-1β) and tumor necrosis factor α(TNF-α) from human peripheral mononuclear cells, lymphocytes andmonocytes stimulated by heat-killed Acitnobacillus actinomycetemcomitans (ATCC 29523), Porphyromonas gingivalis (ATCC 33277) and Prevotella intermedia (ATCC 25611), and also by their sonicated bacterial extracts(SBE), respectively. The effects of three strains of heat-killed bacteria and their SBEs on the morphology of cultured blood cell lines HL-60 (KCLB 10240) and J774A.1 (KCLB 40067) were observed under the inverted microscope. Ultrastructural changes of J774A.1 exposed to heat- killed P. intermedia and its SBE were investigated using transmission electron microscopy. Production of IL-1β was reduced in human peripheral mononuclear cells after stimulation by sonic bacterial extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. Heat- killed and sonic extract of P. gingivalis inhibited the production of TNF-α in peripheral mononuclear cells. Production of TNF-α was inhibited in peripheral monocytes after stimulation by sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. HL-60 and J 774A.1 cells showed granular degeneration after treatment with heat- killed and sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia Chromatin margination and shrinkage were observed in 774A.1 treated with heat-killed P. intermedia. Cell wall structure and organelles were destroyed and vacuoles were formed in cytoplasm in J774A.1 treated with P. intermedia sonic extract. These results suggest that A actinomycetemcomitans, P gingivalis and P intermedia may have an important role in the formation and progression of pulpal diseases via both modulation of production of IL-1β and TNF-α from blood mononuclear cells and cytopathic effects.

세균액 및 세균단백질 추출물이 배양 세포에 미치는 영향

유영대,임미경 대한치과보존학회 2000 Restorative Dentistry & Endodontics Vol.25 No.4

Dental pulp infection is most commonly caused by extensive dental caries, and some bacterial species invade root canals; bacterial components and products are thought to be associated with the pathogenesis of periapical periodontitis. A principle driving force behind pulpal disease response appears to lie in the host immune system's to bacteria and their products. We examined the production of interleukin 1β (IL-1β) and tumor necrosis factor α(TNF-α) from human peripheral mononuclear cells, lymphocytes and monocytes stimulated by heat-killed Acitnobacillus actinomycetemcomitans (ATCC 29523), Porphyromonas gingivalis (ATCC 33277) and Prevotella intermedia (ATCC 25611), and also by their sonicated bacterial extracts(SBE), respectively. The effects of three strains of heat-killed bacteria and their SBEs on the morphology of cultured blood cell lines HL-60 (KCLB 10240) and J774A.1 (KCLB 40067) were observed under the inverted microscope. Ultrastructural changes of J774A.1 exposed to heat- killed P. intermedia and its SBE were investigated using transmission electron microscopy. Production of IL-1β was reduced in human peripheral mononuclear cells after stimulation by sonic bacterial extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. Heat- killed and Sonic extract of P. gingivalis inhibited the production of TNF-α in peripheral mononuclear cells. Production of TNF-α was inhibited in peripheral monocytes after stimulation by sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. HL-60 and J 774A.1 cells showed granular degeneration after treatment with heat- killed and sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia Chromatin margination and shrinkage were observed in 774A.1 treated with heat-killed P. intermedia. Cell wall structure and organelles were destroyed and vacuoles were formed in cytoplasm in J774A.1 treated with P. intermedia sonic extract. These results suggest that A. actinomycetemcomitans, P. gingivalis and P. intermedia may have an important role in the formation and progression of pulpal diseases via both modulation of production of IL-1β and TNF-α from blood mononuclear cells and cytopathic effects.

수산화칼슘의 L929 세포독성 및 연쇄구균에 대한 항균효과에 관한 연구

임미경,유영대 大韓齒科保存學會 1995 Restorative Dentistry & Endodontics Vol.20 No.2

수산화칼슘의 L929 세포독성 및 연쇄구균에 대한 항균효과에 관한 연구

유영대,임미경 원광대학교 치의학연구소 1995 圓光齒醫學 Vol.5 No.2

Calcium hydroxide has been used not only as pulp capping and pulpotomy agents in the operative dentistry, but dressing and temporary filling materials in root canal treatment. Calcium hydroxide was known to stimulate odontoblast to produce new reparative dentin and to eliminate microroganims effectvely in infected root canals. The purpose of this study was to evaluate the effect of calcium hydroxide solution on cultured L929 cells and its antibacterial effect on several streptococci. Calcium hydroxide solution (0.121 g/100㎖) was added to L929 cells and cell viability was measured using 3-(4,5-dimethylthiazol-2-yl)-2, 5-dimethyltetrazolium bromide (MTT) and neutral red (NR) dye. Calcium hydroxide solution (20, 40, 60, 80, 100 and 150㎕) was exposed to L929 cells in 96-well microplates for 1, 4 and 24 hours respectively. Cell viability was gradually decreased when the volume and exposure time of calcium hydroxide solution were increased. When 150ge of calcium hydroxide was applied to L929 cells for 24 hours, there were more than fifty percent reduction of cell viability. Calcium hydroxide solution (20g/100㎖) showed antibacterial effect against S. uberis, S. intermedius and S. mitis after thirty- second exposure. But 0.121g/100㎖ concentration of cacium hydroxide solution exhibited no antibacterial effect on six streptococci after one-hour exposure.