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RISS 인기검색어
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- 저자 : 원찬욱 ( Chan Wook Won ) (검색결과 2 건)
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Human Aortic Smooth Muscle Cell에서 하엽(荷葉)의 항동맥경화 활성 연구
김선모 ( Sun Mo Kim ),윤현정 ( Hyun Jeong Yun ),이효승 ( Hyo Seung Yi ),원찬욱 ( Chan Wook Won ),김재은 ( Jai Eun Kim ),박선동 ( Sun Dong Park ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.4
Objectives: The proliferation and migration of human aortic smooth muscle cells (HASMC) in response to activation by various stimuli plays a critical role in the initiation and development of atherosclerosis. This study was conducted to examine the effects of Nelumbo nucifera leaves (NNL) on the proliferation and migration of HASMC. Additionally, the mechanisms involved in any observed effects were also evaluated. Methods: Apoptotic cells were measured by staining with FITC-labeled annexin V, followed by flow cytometric analysis. The expression level of apoptosis related proteins was confirmed by western blot. And MMP-9 activity was measured by gelatin zymography and MMP-9 expression was measured by ELISA Results: NNL completely inhibited the proliferation of HASMC via induction of the expression of apoptotic proteins including annexin V, cleaved poly ADP-ribose polymerase (PARP), and caspase-3 and -8. NNL treatment resulted in the release of cytochrome c into cytosol, a loss of mitochondrial membrane potential, a decrease in Bcl-2 and Bcl-xL and an increase in Bax expression. NNL also blocked HASMC migration via suppression of MMP-9. Conclusions: Taken together, these results indicate that NNL has the potential for use as an anti-artherosclerosis agent.
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혈관내피세포에서 토복령(土茯령)의 항산화 및 항염증 효과
이창현 ( Chang Hyun Lee ),이효승 ( Hyo Seung Yi ),김재은 ( Jae Eun Kim ),허숙경 ( Sook Kyoung Heo ),차창민 ( Chang Min Cha ),원찬욱 ( Chan Wook Won ),박선동 ( Sun Dong Park ) 대한본초학회 2009 大韓本草學會誌 Vol.24 No.3
Objectives: Smilacis glabrae rhizoma (SG) has been traditionally used as a herbal medication of musculoskeletal disorders like arthritis, pain, convulsions, and syphilis in traditional Korean medicine. This study was investigated anti-oxidative and anti-inflammatory effect of fractionated extracts of Smilacis Glabrae Rhizoma in Human Umbilical Vein Endothelial Cell (HUVEC). Methods: SG extract prepared with methanol, and then fractionated with hexane, dichloromethane, ethylacetate, n-butanol and water. Inhibitory effect of SG onto free radical generation was determined by measuring DPPH, superoxide anions and nitric oxide scavenging activities in vitro. Cytotoxic activity of extracts on RAW 264.7 cells was measured using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. Intracelluar oxidation was analysed by DCF-DA assay. The nitric oxide (NO) production was measured by Griess reagent system. The levels of ICAM-1 and VCAM-1 expression were confirmed by western blot. And proinflammatory cytokines were measured by ELISA kit. Results: Our results indicated that fractionated extracts, especially ethyl acetate (EA) extract, significantly inhibited free radical generation, the TNF-α-induced intracellular oxidation. Furthermore, the EA extract protected TNF- α-induced adhesion to THP-1, expression of adhesion molecules accompanied by an attenuation of IL-6 and IL-8 formation in HUVEC. Conclusions: These results indicate that EA extract of SG have potential as an agent of atherosclerosis and other chronic inflammatory diseases including diabetes, hypertension, and arthritis.
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