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      • KCI우수등재

        면양의 제1위내 미생물 단백질 함량에 미치는 요인과 소장에서 아미노산이용에 관한 연구 1 . 제1위 미생물 단백질합성

        이남형,암스트롱 ( Nam Hyung Lee,D . G . Armstrong ) 한국축산학회 1985 한국축산학회지 Vol.27 No.7

        This experiment was conducted to examine the effects of different carbohydrate source (barley and maize) and nitrogen source (soyabean meal and meat and bone meal) upon rumen microbial protein synthesis as well as nitrogen flow in the small intestine of sheep given diets containing grains and alkali treated barley straw (approximately 74:26). The results obtained are as follows: 1) No significant differences (p$gt;0.05) between diets were observed in the mean values obtained for pH, VFA concentrations and the molar proportions of acetic, propionic, and butyric acids. NH³-N concentration in rumen fluid were non significantly lower (p$gt;0.05) for the maize-soya diet than the other diets. 2) No significant differences (p$gt;0.05) between diets were observed in the mean values obtained for rumen volume, rumen dilution rate, and rumen retention time. Rumen dilution rate (h^(-1)) for the barley-meat and bone, barley-soya, maize-meat and bone, and maize-soya diets were estimated as 0.043, 0.046, 0.036 and 0.048 respectively. 3) There were significant differences between diets in the quantities of DM and OM passing at the proximal duodenum and terminal ileum (p$lt;0.05) and in the faeces (p$lt;0.01 for DM and p$lt;0.05 for OM). However, there were no significant differences (p$gt;0.05) between diets in the apparently digested DM and OM disappearing before the small intestine and the whole digestive tract. The mean values for apparent OM digestibility and the proportion of the apparently digested OM disappearing prior to and within the small intestine for four diets were 0.8246±0.0168, 0.6717±0.238, and 0.2351±0.0247 respectively. 4) No significant differences (p$gt;0.05) between diets were observed for the total N and NAN entering the small intestine, total N in the urine, retained N, and proportionate disappearance apparently digested N within the small intestine and the whole digestive tract. It was non significant net gain in the quantity of total N in the passage of the digesta through the stomach for all diets (1.107±0.035 of mean values). But there were significant differences between diets in the quantities of total N leaving the small intestine (p$lt;0.01) and N absorbed in the whole digestive tract (p$lt;0.01). 5) Proportion of microbial N entering the small intestine estimated by using S^(35) infusion techniques were 0.4998 for barley-meat and bone meal diet, 0.5269 for barley-Soya diet, 0.4512 for maize-meat and bone diet, and 0.5384 for maize-Soya diet. The dietary N degradation in the rumen for the diet containing meat and bone meal was non significantly lower (p$gt;0.05) than the value for the diet with soyabean meal. 6) The efficiencies of microbial N synthesis within the rumen, expressed as 9 microbial N per ㎏ organic matter apparently digested in the rumen (OMAPDR) or organic matter truly digested in the rumen (OMTDR), were shown no significant differences between diets. The efficiencies of microbial N synthesis for barley-meat and bone diet, barley-Soya diet, maize-meat and bone diet, and maize-soya diet were estimated as 16.79, 16.30, 15.37 and 17.58 gN/㎏ OMTDR respectively or 22.71, 21.21, 20.36 and 24.21 gN/㎏ OMAPDR respectively.

      • KCI우수등재

        면양의 제1위내 미생물 단백질합성에 미치는 요인과 소장에서 아미노산 이용에 관한 연구 2 . 소장에서 아미노산 이용

        이남형,암스트롱 ( Nam Hyung Lee,D . G . Armstrong ) 한국축산학회 1985 한국축산학회지 Vol.27 No.8

        This experiment was conducted to examine the effects of different carbohydrate source and nitrogen source upon the utilization of amino acids in the small intestine of sheep. The results obtained are as follows: 1) For the quantities of TAA-N, EAA-N and NEAA-N passing at the duodenum and ileum except NEAA-N passing at the ileum were no significant differences (p$gt;0.05) between diets. The quantities of NEAA-N passing at the ileum for the diets containing meat and bone meal were significantly higher (P$lt;0.05) than the diets containing soyabean meal. The mean values for the proportionate disappearance apparently digested TAA-N, EAA-N and NEAAN within the small intestine for four diets were 0.692±0.0449, 0.702±0.0132, and 0.682±0.726, respectively. 2) There were no significant differences (p$gt;0.05) in duodenal individual amino acid flow between diets with the exception of aspartic acid and glycine. The amounts of each amino acid in duodenal digesta, expressed as a proportion of the amounts ingested for the four diets, were shown that there were net gains of EAA with the exception of arginine and NEAA with the exception of glutamic acid, glycine and proline prior to the small intestine. 3) Within the small intestine, there were no significant losses of each EAA (p$gt;0.05) but significant losses of aspartic acid and glycine of NEAA between diets (p$lt;0.01). The mean values of the proportionate losses of methionine, alanine and lysine within the small intestine were 0.816±0.04, 0.767±0.04, and 0.732±0.01, respectively.

      • KCI우수등재

        개미산 또는 개미산과 훠름알데하이드를 처리한 목초 싸일레지가 Jersey 미경산우의 제1위내 미생물 단백질 합성과 단백질 분해율에 미치는 영향

        이남형,루크,암스트롱 ( Nam Hyung Lee,J . A . Rooke,D . G . Armstrong ) 한국축산학회 1985 한국축산학회지 Vol.27 No.8

        The aim of the experiment was to assess the effect of applying formic acid at ensilage or formic acid/formaldehyde to the standing crop of herbage before ensiling upon the subsequent digestion of the silages by Jersey heifers. Unwilted grass silages were made from perennial rye; rass ensiled at a DM of 220㎏^(-1) with application of 2.22 liters ton of formic acid and wilted grass silages was made by spray to the standing crop of herbage with 90 liters ㏊^(-1) of a solution containing 50 liter formalin, 500㎖ of 10% Tween 20, and 2.5 liter water giving an application rate of 61g formaldehyde ㎏^(-1) herbage crude protein. The silages were fed to cattle equipped with rumen cannulae and duodenal re-entrant cannulae. Results from fermentation in the rumen and from the digestion of organic matter showed major differences between silages. The efficiency of rumen microbial nitrogen synthesis was also affected by additive treatment. Microbial N synthesized per ㎏ organic matter apparently digested in the rumen was 20g for the formic acid silage and 17.68 for the formic acid/formaldehyde silage. Degradability of feed N in the rumen was similar for two silages.

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