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        경기도 포천, 강원도 철원지역에서 진드기의 계절적 발생 유형

        신용칠 ( Yong Chil Shin ),이인용 ( In Yong Lee ),서장훈 ( Jang Hoon Seo ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.3

        The seasonal pattern of larvae, nymph and adult life stages for three tick species were studied for April-October, 2014. Haemaphysalis longicornis (610), Haemaphysalis flava (296) and Ixodes nipponensis (15) were collected from Pocheon by the dragging and flagging method. H.longicornis (254), H.flava (345) and I.nipponensis (15) were collected from Cheolwon in the same way. H.longicornis adults peaked in June while nymphs were more frequently collected from April through June. In contrast, H.longicornis larvae were collected only in September and October. H.flava adults were collected in April, September, and October while nymphs were collected from April through October. The nymphs peaked in April in Pocheon while May was the peak inCheolwon. Larvae of H.longicornis and H.flava were collected only in September and October. I.nipponensis adults and nymphs were more frequently collected in April, while the larvae were not collected during that period. Collecting ticks by the dragging and flagging method provides estimates of population densities for life stages of the selected species and the associated disease risk. These results can be used to predict seasonal population densities of ticks and the prevalence of tick-borne pathogens.

      • 중추 및 말초신경계 종양조직에서의 c-myc, c-H-ras 관련단백 및 표피성장인자에 관한 면역조직학적 연구

        이상수,신용칠,염범우 고려대학교 의과대학 1993 고려대 의대 잡지 Vol.30 No.1

        Activated oncogenes and oncoproteins are detected in various malignant tumors and it is believed that the altered expression of oncogenes and oncoproteins plays an important role in the development of neoplasm. The tumors of the nervous system have some characteristics such as low metastatic potential, frequent association with other clinical signs and early development or congenital in origin. Normally many oncogenes are present in the brain tissue, but there are many differences in level depending on the sites and on the periods of embryo, childhood and adult. So we had carried an immunohistochemical study to understand the presence of c-myc, c-H-ras products and epidermal growth factor (EGF) on tumor cells of the nervous system using formalin fixed and paraffin embedded tissue blocks of 30 tumor cases of the central nervous system and 29 cases of the peripheral nervous system. The results are as followings; 1. The highest ratio of positive reaction in all tumors of nervous system is noted in astrocytoma and the lowest ratio is in malignant schwannoma. 2. The positivity against three antiserum is higher in the tumors of central nervous system than those of peripheral nervous system. 3. Among 3 oncoprotein antiserum, c-myc showed the highest positive ratio and c-H-ras was the lowest in positivity.

      • 난소의 생식세포 기원성 종양세포에서의 c-myc, c-H-res 관련 단백 및 표피성장인자에 관한 면역조직학적 연구

        현동관,신용칠,염범우 고려대학교 의과대학 1993 고려대 의대 잡지 Vol.30 No.1

        The causes of malignant tumor include chemical agents, irradiation, viral infection and others. Among them, oncogenic viral infection clarified the pathogenesis of the neoplasm. Oncogenic virus has viral oncogene which has transforming activity of cells when it is incoorperated into the DNA of the host cells. These DNA of transformed cells also have the activity of transformation of recipient cells and production of infectious virus, in culture. The normal cells may have the DNA showing homologous or similar structure of viral oncogene. These cellular oncogenes may be activated by gene mutation chromosomal translocation, deletion and promotor insertion. In these cases, abnormal oncogene related proteins are produced, such as ras P21 and P62-c-myc proteins. There are many reports that oncogene related proteins are detected in variable carcinomas, sarcomas, and leukemia-lymphomas. However, there are few reports on germ cell tumors of ovary So we stained rasP21 (ras gene related protein), P62-c-myc (c-myc related protein) and EGF antisera on various ovarian germ cell tumors with the method of avidin-biotin peroxidase complex. The results are as followings, 1. The positive reaction of all three antisera against the oncoprotein is the highest in cystic teratoma. 2. In cystic teratomas, the squamous cells and sebaceous glands show the strongest positive reaction and chondroid and glial tissue shows the weakest positive reaction. 3. c-myc antiserum shows the strongest positive reaction in the most tumors comparing with that of other two antisera.

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