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꽃사슴(Formosan deer)의 Hemoglobin형(型)에 관한 연구(硏究)
임영재,스즈키 쇼조,Lim, Young-jae,Suzuki, Shozo 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.1
The hemoglobin phenotype and the gene frequencies of 44 Formosan deer(Cervus nippon) in Kyung-Gi area were examined by using cellulose acetate and starch gel electrophoresis. 1. The method of cellulose acetate electrophoresis was simplier, more clear and preserative than starch gel electrophoresis. 2. The hemoglobin phenotype was appeared 3 types as $Hb^F$ $Hb^{FS}$ and $Hb^S$. The frequencies of appearance were $Hb^F$ 47.7%, $Hb^{FS}$ 47.7% and $Hb^S$ 4.5%, respectively. 3. The genetic factors of hemoglobin were observed as $Hb^F$ and $Hb^S$ and the rates of gene frequencies were 71.6% and 28.4%, respectively.
꽃사슴의 혈액형(血液型)에 관한 연구(硏究) I. 면역반응(免疫反應)에 의한 꽃사슴의 혈액형분류(血液型分類)(제일보(第一報))
임영재,스즈키 쇼조,Lim, Young-Jae,Suzuki, Shozo 대한수의학회 1983 大韓獸醫學會誌 Vol.23 No.1
The present study was conducted to clarify the presence or absence of isohemagglutinin and isohemolysin and, if present, to classify blood types in the Formosan deer. It was found that, though the titers were relatively low, isohemagglutinin and isohemolysin were present in the deer sera. Results obtained before and after heat treatment of the deer sera at $56^{\circ}C$ for 30 minutes were variable, some stable and some unstable to the treatment and still some showing the activities only after the heat treatment. Rabbits, when immunized with deer blood cells, responded with very high titers both in agglutination and hemolysis tests and the activities were not inactivated by the treatment of the immune sera at $56^{\circ}C$ for 30 minutes. Using four rabbit immune sera to different deer blood cells following blood types were recognized among 30 heads of deer; 1. by hemagglutination test A : positive to all four immune sere (13 heads). B : positive to the immune sera of 7BS and 8BS but negative to those of 1BS and 3BS (8 heads). C : positive to the sera of 3BS, 7BS and 8BS but negative to that of 1BS (9 heads). 2. by hemolysis test A : positive to all four immune sera (24 heads). B : positive to the immune sera of 7BS and 8BS but negative to those of 1BS and 3BS (3 heads). C : positive to the sera of 3BS, 7BS and 8BS but negative to that of 1BS (3 heads).
Studies on the Blood Protein Polymorphisms of Deer: Cervus nippon, Cervus unicolor
임영재,송영의,스즈키 쇼조,타나카 카즈,아마노 타카시,쿠로사와 야츠,카츠마타 마코토,Lim, Young-jae,Song, Yung-yi,Suzuki, Shozo,Thanaka, Kazue,Amano, Takashi,Kurosawa, Yaetsu,Katsumata, Makoto The Korean Society of Veterinary Science 1985 大韓獸醫學會誌 Vol.25 No.1
한국(韓國), 대만(臺灣), 일본(日本)에서 사육(飼育)하고 있는 꽃사슴(Cervus nippon) 129두(頭)와 물사슴(Cervus unicolor) 7두(頭)에서 hemoglobin, transferrin, albumin carbonic anhydrase, slow-${\alpha}_2$ 및 amylase형(型)을 전기영동(電氣泳動)에 의(依)하여 분석(分析)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1. cellulose acetate에 의(依)한 전기영동(電氣泳動)이 starch gel에 의(依)한 전기영동(電氣泳動)보다 hemoglobin형(型) 분리(分離)에 있어서 더 간편하며 시간이 적게 걸리고, 선명(鮮明)할 뿐만 아니라 영구보존(永久保存)이 가능(可能)하다. 2. 꽃사슴의 hemoglobin형(型)은 $Hb^F$, $Hb^{FS}$, $Hb^S$형(型)으로 분리(分離)되었으나 물사슴에 있어서는 전부(全部) $Hb^F$형(型)으로 나타났다. 3. hemoglobin ${\beta}$ chain은 4가지형(型) 즉 ${\beta}$-1, ${\beta}$-2, ${\beta}$-3 및 ${\beta}$-4로 분리(分離)되었다. 4. hemoglobin ${\alpha}$ chain은 ${\alpha}_1$과 ${\alpha}_1{\alpha}_2$형(型)으로 분리(分離)되었다. 5. slow-${\alpha}_2$형(型)은 A형(型)과 AB형(型)으로 분리(分離)되었으며, 꽃사슴에 있어서는 AB형(型)이 12% 출현(出現)하였으나 물사슴에서는 전부(全部) A형(型)으로 AB형(型)은 없었다. 6. albumin형(型)에서는 F형(型)과 S형(型)으로 분리(分離)되었으며 꽃사슴은 전부(全部) F형(型)이였고, 물사슴은 전부(全部) S형(型)이였다. 7. transferrin형(型), carbonic anhydrase형(型) 및 amylase형(型)은 전부(全部) 각각(各各) 1종류(種類)의 형(型)이였다.